LOCUS       sequence-162824-         915 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 915)
  AUTHORS   Sarbassov DD, Guertin DA, Ali SM, Sabatini DM
  TITLE     Phosphorylation and regulation of Akt/PKB by the rictor-mTOR 
            complex.
  JOURNAL   Science 2005 Feb 18;307(5712):1098-101.
  PUBMED    15718470
REFERENCE   2  (bases 1 to 915)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Science"; 
            date: "2005-02-18"; volume: "307(5712)"; pages: "1098-101"
COMMENT     Sequence Label: mTOR_2 shRNA
FEATURES             Location/Qualifiers
     source          1..915
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_feature    136..253
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        302..812
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     731..749
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     primer_bind     complement(834..853)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
ORIGIN
        1 tggctttata tatcttgtgg aaaggacgaa acaccggccg cattgtctct atcaagttct
       61 tcctgtcaaa cttgatagag acaatgcggt ttttgaattc tcgacctcga gacaaatggc
      121 agtattcatc cacaatttta aaagaaaagg ggggattggg gggtacagtg caggggaaag
      181 aatagtagac ataatagcaa cagacataca aactaaagaa ttacaaaaac aaattacaaa
      241 aattcaaaat tttcgggttt attacaggga cagcagagat ccactttggc cgcggctcga
      301 gggggttggg gttgcgcctt ttccaaggca gccctgggtt tgcgcaggga cgcggctgct
      361 ctgggcgtgg ttccgggaaa cgcagcggcg ccgaccctgg gtctcgcaca ttcttcacgt
      421 ccgttcgcag cgtcacccgg atcttcgccg ctacccttgt gggccccccg gcgacgcttc
      481 ctgctccgcc cctaagtcgg gaaggttcct tgcggttcgc ggcgtgccgg acgtgacaaa
      541 cggaagccgc acgtctcact agtaccctcg cagacggaca gcgccaggga gcaatggcag
      601 cgcgccgacc gcgatgggct gtggccaata gcggctgctc agcagggcgc gccgagagca
      661 gcggccggga aggggcggtg cgggaggcgg ggtgtggggc ggtagtgtgg gccctgttcc
      721 tgcccgcgcg gtgttccgca ttctgcaagc ctccggagcg cacgtcggca gtcggctccc
      781 tcgttgaccg aatcaccgac ctctctcccc agggggatcc accggagctt accatgaccg
      841 agtacaagcc cacggtgcgc ctcgccaccc gcgacgacgt ccccagggcc gtacgcaccc
      901 tcgccgccgc gttcg
//
