LOCUS       sequence-162839-         999 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Insert is a fragment of the Bmpr2 3'-UTR that contains a mutated 
            miR-19 binding site. To be used for 3'UTR assay..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 999)
  AUTHORS   Murphy BL, Obad S, Bihannic L, Ayrault O, Zindy F, Kauppinen S, 
            Roussel MF
  TITLE     Silencing of the miR-17~92 cluster family inhibits medulloblastoma 
            progression.
  JOURNAL   Cancer Res. 2013 Oct 21.
  PUBMED    24145352
REFERENCE   2  (bases 1 to 999)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cancer Res.
            2013 Oct 21."
COMMENT     Sequence Label: pmirGLO-Bmpr2-Delta-19
FEATURES             Location/Qualifiers
     source          1..999
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(221..239)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     polyA_signal    276..397
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(287..306)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     341..360
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     promoter        642..999
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        642..833
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(665..685)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      850..985
                     /label=SV40 ori
                     /note="SV40 origin of replication"
ORIGIN
        1 ggactgaccg gcaagttgga cgcccgcaag atccgcgaga ttctcattaa ggccaagaag
       61 ggcggcaaga tcgccgtgta attctagttg tttaaactag cggccgctag ttttgttttt
      121 taagtttacc acttttgttt agtctagagt cgacctgcag gcatgcaagc tgatccggct
      181 gctaacaaag cccgaaagga agctgagttg gctgctgcca ccgctgagca ataactagca
      241 taaccccttg gggcggccgc ttcgagcaga catgataaga tacattgatg agtttggaca
      301 aaccacaact agaatgcagt gaaaaaaatg ctttatttgt gaaatttgtg atgctattgc
      361 tttatttgta accattataa gctgcaataa acaagttaac aacaacaatt gcattcattt
      421 tatgtttcag gttcaggggg agatgtggga ggttttttta agcaagtaaa acctctacaa
      481 atgtggtaaa atcgaatttt aacaaaatat taacgcttac aatttcctga tgcggtattt
      541 tctccttacg catctgtgcg gtatttcaca ccgcatacgc ggatctgcgc agcaccatgg
      601 cctgaaataa cctctgaaag aggaacttgg ttaggtacct tctgaggcgg aaagaaccag
      661 ctgtggaatg tgtgtcagtt agggtgtgga aagtccccag gctccccagc aggcagaagt
      721 atgcaaagca tgcatctcaa ttagtcagca accaggtgtg gaaagtcccc aggctcccca
      781 gcaggcagaa gtatgcaaag catgcatctc aattagtcag caaccatagt cccgccccta
      841 actccgccca tcccgcccct aactccgccc agttccgccc attctccgcc ccatggctga
      901 ctaatttttt ttatttatgc agaggccgag gccgcctcgg cctctgagct attccagaag
      961 tagtgaggag gcttttttgg aggcctaggc ttttgcaaa
//
