LOCUS       sequence-162847-         984 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Insert is a fragment of the Smad5 3'-UTR that contains miR-17 and 
            miR-19 binding sites. To be used for 3'UTR assay..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 984)
  AUTHORS   Murphy BL, Obad S, Bihannic L, Ayrault O, Zindy F, Kauppinen S, 
            Roussel MF
  TITLE     Silencing of the miR-17~92 cluster family inhibits medulloblastoma 
            progression.
  JOURNAL   Cancer Res. 2013 Oct 21.
  PUBMED    24145352
REFERENCE   2  (bases 1 to 984)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cancer Res.
            2013 Oct 21."
COMMENT     Sequence Label: pmirGLO-Smad5-1719
FEATURES             Location/Qualifiers
     source          1..984
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(228..246)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     polyA_signal    283..404
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(294..313)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     348..367
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     enhancer        649..840
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(672..692)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
ORIGIN
        1 ggactgaccg gcaagttgga cgcccgcaag atccgcgaga ttctcattaa ggccaagaag
       61 ggcggcaaga tcgccgtgta attctagttg tttaaactag cggccgctag tattaatctt
      121 ttcattttgc acttttatgg gtgactgtct ctagagtcga cctgcaggca tgcaagctga
      181 tccggctgct aacaaagccc gaaaggaagc tgagttggct gctgccaccg ctgagcaata
      241 actagcataa ccccttgggg cggccgcttc gagcagacat gataagatac attgatgagt
      301 ttggacaaac cacaactaga atgcagtgaa aaaaatgctt tatttgtgaa atttgtgatg
      361 ctattgcttt atttgtaacc attataagct gcaataaaca agttaacaac aacaattgca
      421 ttcattttat gtttcaggtt cagggggaga tgtgggaggt ttttttaagc aagtaaaacc
      481 tctacaaatg tggtaaaatc gaattttaac aaaatattaa cgcttacaat ttcctgatgc
      541 ggtattttct ccttacgcat ctgtgcggta tttcacaccg catacgcgga tctgcgcagc
      601 accatggcct gaaataacct ctgaaagagg aacttggtta ggtaccttct gaggcggaaa
      661 gaaccagctg tggaatgtgt gtcagttagg gtgtggaaag tccccaggct ccccagcagg
      721 cagaagtatg caaagcatgc atctcaatta gtcagcaacc aggtgtggaa agtccccagg
      781 ctccccagca ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa ccatagtccc
      841 gcccctaact ccgcccatcc cgcccctaac tccgcccagt tccgcccatt ctccgcccca
      901 tggctgacta atttttttta tttatgcaga ggccgaggcc gcctcggcct ctgagctatt
      961 ccagaagtag tgaggaggct tttt
//
