LOCUS       sequence-162848-        1054 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Insert is a fragment of the Smad5 3'-UTR that contains miR-17 and 
            miR-19 binding sites. To be used for 3'UTR assay..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1054)
  AUTHORS   Murphy BL, Obad S, Bihannic L, Ayrault O, Zindy F, Kauppinen S, 
            Roussel MF
  TITLE     Silencing of the miR-17~92 cluster family inhibits medulloblastoma 
            progression.
  JOURNAL   Cancer Res. 2013 Oct 21.
  PUBMED    24145352
REFERENCE   2  (bases 1 to 1054)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cancer Res.
            2013 Oct 21."
COMMENT     Sequence Label: pmirGLO-Smad5-1719
FEATURES             Location/Qualifiers
     source          1..1054
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(91..1023)
                     /codon_start=1
                     /product="Renilla luciferase"
                     /label=hRluc
                     /note="human codon-optimized"
                     /translation="MASKVYDPEQRKRMITGPQWWARCKQMNVLDSFINYYDSEKHAEN
                     AVIFLHGNAASSYLWRHVVPHIEPVARCIIPDLIGMGKSGKSGNGSYRLLDHYKYLTAW
                     FELLNLPKKIIFVGHDWGACLAFHYSYEHQDKIKAIVHAESVVDVIESWDEWPDIEEDI
                     ALIKSEEGEKMVLENNFFVETMLPSKIMRKLEPEEFAAYLEPFKEKGEVRRPTLSWPRE
                     IPLVKGGKPDVVQIVRNYNAYLRASDDLPKMFIESDPGFFSNAIVEGAKKFPNTEFVKV
                     KGLHFSQEDAPDEMGKYIKSFVERVLKNEQ"
     regulatory      1020..1029
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 agcggccgga gaacctgcgt gcaatccatc ttgttcaatc cctccggagc ctccgccacc
       61 tgatccgcca cctccgctcc caccaccggt ctgctcgttc ttcagcacgc gctccacgaa
      121 gctcttgatg tacttaccca tttcatctgg agcgtcctcc tggctgaagt ggaggccctt
      181 caccttcacg aactcggtgt tagggaactt cttagctccc tcgacaatag cgttggaaaa
      241 gaacccaggg tcggactcga tgaacatctt aggcagatcg tcgctggccc gaaggtaggc
      301 gttgtagttg cggacaatct ggacgacgtc gggcttgcct cccttaacga gagggatctc
      361 gcgaggccag gagagggtag gccgtctaac ctcgcccttc tccttgaatg gctccaggta
      421 ggcagcgaac tcctcaggct ccagtttccg catgatcttg cttgggagca tggtctcgac
      481 gaagaagtta ttctcaagca ccattttctc gccctcttcg ctcttgatca gggcgatatc
      541 ctcctcgatg tcaggccact cgtcccagga ctcgatcacg tccacgacac tctcagcatg
      601 gacgatggcc ttgatcttgt cttggtgctc gtaggagtag tgaaaggcca gacaagcccc
      661 ccagtcgtgg cccacaaaga tgattttctt tggaaggttc agcagctcga accaagcggt
      721 gaggtacttg tagtgatcca ggaggcgata tgagccattc ccgctcttgc cggacttacc
      781 cattccgatc agatcaggga tgatgcatct agccacgggc tcgatgtgag gcacgacgtg
      841 cctccacagg tagctggagg cagcgttacc atgcagaaaa atcacggcgt tctcggcgtg
      901 cttctcggaa tcatagtagt tgatgaagga gtccagcacg ttcatttgct tgcagcgagc
      961 ccaccactga ggcccagtga tcatgcgttt gcgttgctcg gggtcgtaca ccttggaagc
     1021 catggtggct ccagcctttg gttcgagact gttg
//
