LOCUS       sequence-162850-        9519 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9519)
  AUTHORS   Monticelli S, Rao A
  TITLE     NFAT1 and NFAT2 are positive regulators of IL-4 gene transcription.
  JOURNAL   Eur J Immunol. 2002 Oct . 32(10):2971-8.
  PUBMED    12355451
REFERENCE   2  (bases 1 to 9519)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Eur J 
            Immunol. 2002 Oct . 32(10):2971-8."
COMMENT     Sequence Label: WT NFAT2
FEATURES             Location/Qualifiers
     source          1..9519
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    107..128
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(143..148,149..166,167..173)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    181..197
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     LTR             359..874
                     /label=3' LTR
                     /note="3' long terminal repeat from murine embryonic stem 
                     cell virus"
     CDS             complement(join(921..1634,1635..1637,1638..1640))
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     ANFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(966..987)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     1315..1334
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     1574..1595
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     misc_feature    1652..2204
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     misc_feature    1654..2220
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(1814..1833)
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     primer_bind     2043..2060
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     protein_bind    2252..2276
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     primer_bind     2381..2398
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     2382..2398
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     2408..2427
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        2408..2426
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             complement(5209..5298)
                     /codon_start=1
                     /product="three tandem HA epitope tags"
                     /label=3xHA
                     /translation="YPYDVPDYAGYPYDVPDYAGSYPYDVPDYA"
     protein_bind    complement(5333..5357)
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             complement(5382..5798)
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag 
                     gene lacking the start codon"
     primer_bind     complement(5398..5414)
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     primer_bind     complement(5430..5452)
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
     misc_feature    5865..6206
                     /label=MESV Psi
                     /note="packaging signal of murine embryonic stem cell 
                     virus"
     LTR             6270..6786
                     /label=5' LTR
                     /note="5' long terminal repeat from murine embryonic stem 
                     cell virus"
     primer_bind     complement(6986..7005)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     complement(7349..7368)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     7468..7490
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(7528..7546)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        7614..7718
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7719..7787,7788..8579)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7937..7956)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      8750..9338
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9239..9258
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     9492..9509
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 agcgcccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc
       61 acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc
      121 tcactcatta ggcaccccag gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa
      181 ttgtgagcgg ataacaattt cacaattctc cccagctctt tctgcgctgt ccataatgaa
      241 ctatttcagg agtattggag tatgtggatt caaaacagtg gctaattgag tcaggattac
      301 ttaatattcg actcctaccc tcagaatgtt gttctccaac ttcaagaaac tgttacccat
      361 gaaagacccc cgctgacggg tagtcaatca ctcagaggag accctcccaa ggaacagcga
      421 gaccacaagt cggatgcaaa cagcaagagg ctttattggg aatacgggta cccgggcgac
      481 gcagtctatc ggaggactgg cgcgccgagt gaggggttgt gggctctttt attgagctcg
      541 gggagcagaa gcgcgcgaac agaagcgaga agcgaactga ttggttagtt caaataaggc
      601 acagggtcat ttcaggtcct tggggcaccc tggaaacatc tgatggttct ctagaaactg
      661 ctgagggcgg gaccgcatct ggggaccatc tgttcttggc cctgagccgg ggcaggaact
      721 gcttaccaca gatatcctgt ttggcccata ttctgctgtc tctctgttcc taaccttgat
      781 ctgaacttct ctattctcag ttatgtattt tccatgcctt gcaaaatggc gttacttaag
      841 ctagcttgcc aaacctacag gtggggtctt tcattccccc ctttttctgg agactaaata
      901 aaatctttta ttttatctca ttacttgtac agctcgtcca tgccgagagt gatcccggcg
      961 gcggtcacga actccagcag gaccatgtga tcgcgcttct cgttggggtc tttgctcagg
     1021 gcggactggg tgctcaggta gtggttgtcg ggcagcagca cggggccgtc gccgatgggg
     1081 gtgttctgct ggtagtggtc ggcgagctgc acgctgccgt cctcgatgtt gtggcggatc
     1141 ttgaagttcg ccttgatgcc gttcttctgc ttgtcggcca tgatatagac gttgtggctg
     1201 ttgtagttgt actccagctt gtgccccagg atgttgccgt cctccttgaa gtcgatgccc
     1261 ttcagctcga tgcggttcac cagggtgtcg ccctcgaact tcacctcggc gcgggtcttg
     1321 tagttgccgt cgtccttgaa gaagatggtg cgctcctgga cgtagccttc gggcatggcg
     1381 gacttgaaga agtcgtgctg cttcatgtgg tcggggtagc ggctgaagca ctgcacgccg
     1441 taggtcaggg tggtcacgag ggtgggccag ggcacgggca gcttgccggt ggtgcagatg
     1501 aacttcaggg tcagcttgcc gtaggtggca tcgccctcgc cctcgccaga cacgctgaac
     1561 ttgtggccgt ttacgtcgcc gtccagctcg accaggatgg gcaccacccc ggtgaacagc
     1621 tcctcgccct tgctcaccat ggttgtggcc atattatcat cgtgtttttc aaaggaaaac
     1681 cacgtccccg tggttcgggg ggcctagacg tttttttaac ctcgactaaa cacatgtaaa
     1741 gcatgtgcac cgaggcccca gatcagatcc catacaatgg ggtaccttct gggcatcctt
     1801 cagccccttg ttgaatacgc ttgaggagag ccatttgact ctttccacaa ctatccaact
     1861 cacaacgtgg cactggggtt gtgccgcctt tgcaggtgta tcttatacac gtggcttttg
     1921 gccgcagagg cacctgtcgc caggtggggg gttccgctgc ctgcaaaggg tcgctacaga
     1981 cgttgtttgt cttcaagaag cttccagagg aactgcttcc ttcacgacat tcaacagacc
     2041 ttgcattcct ttggcgagag gggaaagacc cctaggaatg ctcgtcaaga agacagggcc
     2101 aggtttccgg gccctcacat tgccaaaaga cggcaatatg gtggaaaata acatatagac
     2161 aaacgcacac cggccttatt ccaagcggct tcggccagta acgttagggg ggggggcgga
     2221 attcgatatc aagcttatcg ataccgtcat caccactttg tacaagaaag ctgggtctag
     2281 atcggtgcgg gcctcttcgc tattacgcca gctggcgaaa gggggatgtg ctgcaaggcg
     2341 attaagttgg gtaacgccag ggttttccca gtcacgacgt tgtaaaacga cggccagtga
     2401 gcgcgcgtaa tacgactcac tatagggcga attggagctc caccgcggtg gcggccgctc
     2461 tagactgcag actagtggat ccctcgagct acgtcagggg gggggggagc agttaaaaac
     2521 ggtattttcg taaataaaac accctagaaa gttgaaaagg cgagccgatc acttctgaat
     2581 gaaagaagcc attccctaaa tgagtaaact cgagcagaag agcttccatc tgaaagtctc
     2641 acgtcaccag gggacagcat tattcagtgg agacacaccg cacgctgtgt agaagcgtca
     2701 ccatgaggac aacatcatgt atgttcccca gtgaccacgg caggcttatt ttcgaagcta
     2761 cttacaaaac tgcgttgaca aatactttct tctaacccaa agactcacag aggttagata
     2821 tacaccccca gaccgcatca gcccctgagc gcagcagagc tgcttggttg gccgcgctgc
     2881 tgtatttgca aaaggcgagc aagtgatgac cccagcatgc accagtcaca ggaaaacaca
     2941 cccaggcttc gcttttcttt gagggggtgg gcgacttggt cttgtgaata gggctatcgc
     3001 gtggtgtgaa gaggctacag gtaggttttg ttttgttttg ttttgtttac aacaacatcc
     3061 tcttatagcc tcggctgctc agtaaaaacc tcctctcagc tcactctcag agcttaaggt
     3121 tagaaagaca gagttaccat tggcaggaag gtacgtgaaa cgctggtact ggcttctctt
     3181 ccgtttcccg ttgcagacat agaaactgac ttggacgggg ctggttatcc tctggttgcg
     3241 gaaaggtggt atctcaacca ccagggaatt tggcttgcac aggtcccggt cagtctttgc
     3301 ttccatctcc cagacgtggt ggccatctgg agccttctcc acgaaaatga ctttggagtc
     3361 ttgcagaaag ttatggccag acagcaccat cttcttcccg ccgatgactg ggtagctgtc
     3421 tgtgctctgc ttctccacga ggggcagctc ctgggctgac cgctgggaac actcgatagg
     3481 gttcgaggcc acctggagag acagcgtccg gccattgggc tgtgggatgt gaactcggaa
     3541 gaccagcctc accctggtgt tcttcctccc gatgtctgtc tcccctttcc tcagctcaat
     3601 atcagagttt ctgagcttca ggatcccagc acagtcgatg atggctcgca tgttattttc
     3661 tggaagcaac gggatctcca ggactttggt gttggacagg atgatctcgt ggctggtggt
     3721 ggagacggtc ttccccgtga tccggtggac ctggtagaag gcgtggggcc tcagcaggcg
     3781 gtcgtcagcc gtcccaatga acagctgtag cgtgagaggt tcattctcca agtaaccgtg
     3841 tagctgcaca atggggtgtc ctccagctga agccttcaca gccccccggc tgccttccgt
     3901 ctcatagtga gccctgtggt gagacttggg ctgcacctcg atccgaagct cgtatggacc
     3961 agaatgtgac gggagctgcc agtcaagggc aggcaaggat gggctcatat atgatgtcgg
     4021 ggaaagagac ttgggcttcg cccactgata cgaggcctgt ggcaccgaca gatactgctc
     4081 gcaaaaggca cccttccgaa ggtgctggaa ggtgtactcc tcgggtggga agtcagaagt
     4141 gggtggagtg gtgcccaggt cttccccagc tggctctact ttgagggcca cagagggcgc
     4201 atgctccagt gctgtctttc gagacttgat agggacccca tcacccaggt ccagagtgct
     4261 atcggtggtc agggcgttga tggctgccac aatggcagag ctggtatact gggtggtgtt
     4321 accgagccag gtatcttcgg tcacactgac ccgaggggag ccatgggggg atggtgtggg
     4381 tgagtggtgg ggtgagcagg agggctgccg gccattgaga ctgtacttgc gcttgttgca
     4441 gggggacgtg ggccgggagc cgcgggcacc gagccagctc tcctccgtga tgcttgcccg
     4501 aggagaggtg gatggggagt gcctgggcga tcctagcagg tggcaggcac ccaggcttcg
     4561 gggaaaaccc tcctccgggt ccgtggtctt gggagacacg cagggtgact gccacggaga
     4621 ggtctggggg gacgcgtatg ggtaggagta gttggactcg taggaggagg cctcagagtt
     4681 acagcttctg gaggagagac tgctggctgg gctcaggcag gaggggtctc tgtaggcttc
     4741 caggctgggc aggtgcaggg ttgctgtaga cggtgagcgc ttgcagctag gaagtacgtc
     4801 ttccacctcc acgtcgtgga aaaattggcc gctgccatgg tgtaggccca ggtaggaggt
     4861 gatctcgatt ctcggactct ccagagtcgg ggccccgttg ggtctggtgt tgccagagga
     4921 caggaagtat cccgaaggcc cgctgtccac agcccctccg taactggggg gatgattggc
     4981 tgaaggaaca gctgagatac ccggggtgga cgtctggagg tcgtggcatg ctgctggcaa
     5041 ggcagagtgt gctgtgggaa ggggcagggt cgaggtgaca ctaggggaca cataactgta
     5101 gtgttctgca gccgcggcgc ccccgccgct ctgatcgaac tcgaagagga aatcgaagtc
     5161 gaactccggg tcctgctcca gccccgtcat gtcgcggccg cactgagcag cgtaatctgg
     5221 aacgtcatat ggataggatc ctgcatagtc cgggacgtca tagggatagc ccgcatagtc
     5281 aggaacatcg tatgggtaaa agatcatggt ggaagctttc tatctccttc gaagcctgct
     5341 tttttgtaca aacttgtgat gacctcgaga gatctaattc cggcgcctag agaaggagtg
     5401 agggctggat aaagggagga tcgaggcggg gtcgaacgag gaggttcaag ggggagagac
     5461 ggggcggatg gaggaagagg aggcggaggc ttagggtgta caaagggctt gacccaggga
     5521 ggggggtcaa aagccaaggc ttcccaggtc acgatgtagg ggacctggtc tgggtgtcca
     5581 tgcgggccag gtgaaaagac cttgatctta acctgggtga tgaggtctcg gttaaaggtg
     5641 ccgtctcgcg gccatccgac gttaaaggtt ggccattctg cagagcagaa ggtaacccaa
     5701 cgtctcttct tgacatctac cgactggttg tgagcgatcc gctcgacatc tttccagtga
     5761 cctaaggtca aacttaaggg agtggtaaca gtctggccct aattttcaga caaatacaga
     5821 aacacagtca gacagagaca acacagaacg atgctgcagc gctgcagcag acaagacgcg
     5881 cggcttcggt tccaaaccga aagcaaaaat tcagacggag gcgggaactg ttttaggttc
     5941 tcgtctccta ccagaaccac atatcctgac ggggtcggat tccacatcga ctcccttcct
     6001 caggtcgggc cacaaaaacg gcccccaaag tccctgggac gtctcccagg gttgcggccg
     6061 ggtgttcaga actcgtcagt tccaccacgg gtccgccaga tacagagcta gttagctaac
     6121 tagtacagac gcaggcgcaa acattagatg ccggcacaaa cacgcacaaa gacagagaca
     6181 gacacgaaac gaccgctggc cagcttacct cccggcgggg gggtcggtgg tccctgggca
     6241 ggggtctcca aatctcggtg gaacctccaa atgaaagacc cccgaggtgg gcagtcaatc
     6301 aatctgagga gaccctccca aggatcagcg agtccacgat tcggatgcaa acagcaagag
     6361 gctttattgg gaatacgggt acccgggcga cgcagtctat cggaggactg gcgcgccgag
     6421 tgaggggttg tgggctcttt tattgagctc ggggagcaga agcgcgcgaa cagaagcgag
     6481 aagcgaactg attggttagt tcaaataagg cacagggtca tttcaggtcc ttggggcacc
     6541 ctggaaacat ctgatggttc tctagaaact gctgagggcg ggaccgcatc tggggaccat
     6601 ctgttcttgg ccctgagccg gggcaggaac tgcttaccac agatatcctg tttggcccat
     6661 attctgctgt ctctctgttc ctaaccttga tctgaacttc tctattctca gttatgtatt
     6721 ttccatgcct tgcaaaatgg cgttacttaa gctagcttgc caaacctaca ggtggggtct
     6781 ttcattcccc cctttttctg gagactaaat aaaatctttt attttatcta tggctcgtac
     6841 tctataggct tcagctggtg atattgttga gtcaaaacta gagcctggac cactgatatc
     6901 ctgtctttaa caaattggac taatcgcctc tacgccggac gcatcgtggc cggcatcacc
     6961 ggcgccacag gtgcggttgc tggcgcctat atcgccgaca tcaccgatgg ggaagatcgg
     7021 gctcgccact tcgggctcat gagcgcttgt ttcggcgtgg gtatggtggc aggccccgtg
     7081 gccgggggac tgttgggcgc catctccttg catgcaccat tccttgcgcc gtcgttttac
     7141 aacgtcgtga ctgggaaaac cctggcgtta cccaacttaa tcgccttgca gcacatcccc
     7201 ctttcgccag ctggcgtaat agcgaagagg cccgcaccga tcgcccttcc caacagttgc
     7261 gcagcctgaa tggcgaatgg cgcctgatgc ggtattttct ccttacgcat ctgtgcggta
     7321 tttcacaccg catatggtgc actctcagta caatctgctc tgatgccgca tagttaagcc
     7381 agccccgaca cccgccaaca cccgctgacg cgccctgacg ggcttgtctg ctcccggcat
     7441 ccgcttacag acaagctgtg accgtctccg ggagctgcat gtgtcagagg ttttcaccgt
     7501 catcaccgaa acgcgcgaga cgaaagggcc tcgtgatacg cctattttta taggttaatg
     7561 tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa
     7621 cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac
     7681 cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg
     7741 tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc
     7801 tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg
     7861 atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga
     7921 gcacttttaa agttctgcta tgtggcgcgg tattatcccg tattgacgcc gggcaagagc
     7981 aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag
     8041 aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga
     8101 gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg
     8161 cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga
     8221 atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgtagcaatg gcaacaacgt
     8281 tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact
     8341 ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt
     8401 ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg
     8461 ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta
     8521 tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac
     8581 tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta
     8641 aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt
     8701 tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt
     8761 tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt
     8821 gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc
     8881 agataccaaa tactgttctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg
     8941 tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg
     9001 ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt
     9061 cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac
     9121 tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg
     9181 acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg
     9241 gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat
     9301 ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt
     9361 tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg
     9421 attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa
     9481 cgaccgagcg cagcgagtca gtgagcgagg aagcggaag
//
