LOCUS       sequence-162856-        6144 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6144)
  AUTHORS   Khosravi-Far R, White MA, Westwick JK, Solski PA, 
            Chrzanowska-Wodnicka M, Van Aelst L, Wigler MH, Der CJ
  TITLE     Oncogenic Ras activation of Raf/mitogen-activated protein 
            kinase-independent pathways is sufficient to cause tumorigenic 
            transformation.
  JOURNAL   Mol Cell Biol. 1996 Jul . 16(7):3923-33.
  PUBMED    8668210
REFERENCE   2  (bases 1 to 6144)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell 
            Biol. 1996 Jul . 16(7):3923-33."
COMMENT     Sequence Label: pBabe N-Ras 61K
FEATURES             Location/Qualifiers
     source          1..6144
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             120..589
                     /note="long terminal repeat from Moloney murine leukemia 
                     virus"
     CDS             complement(810..1409)
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(893..913)
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     primer_bind     1390..1409
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     promoter        complement(1419..1748)
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      1433..1568
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     1733..1753
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     CDS             complement(2869..3285)
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag 
                     gene lacking the start codon"
     primer_bind     complement(2885..2901)
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     primer_bind     complement(2917..2939)
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
     misc_feature    3486..3685
                     /label=MMLV Psi
                     /note="packaging signal of Moloney murine leukemia virus 
                     (MMLV)"
     LTR             3750..4219
                     /note="long terminal repeat from Moloney murine leukemia 
                     virus"
     promoter        4250..4354
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4355..4423,4424..5215)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4573..4592)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5375..5963
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5864..5883
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6117..6134
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 agcgcccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcaactatg
       61 gccatttaat gtaaatactt aagaaaaaaa accaaattaa ttttgataca tgctgcatgt
      121 gaaagacccc cgctgacggg tagtcaatca ctcagaggag accctcccaa ggaacagcga
      181 gaccacaagt cggatgcaac tgcaagaggg tttattggat acacgggtac ccgggcgact
      241 cagtcaatcg gaggactggc gccccgagtg aggggttgtg ggctctttta ttgagctcgg
      301 ggagcagaag cgcgcgaaca gaagcgagaa gcgaactgat tggttagttc aaataaggca
      361 cagggtcatt tcaggtcctt ggggcaccct ggaaacatct gatggttctc tagaaactgc
      421 tgagggctgg accgcatctg gggaccatct gttcttggcc ctgagccggg gcaggaactg
      481 cttaccacag atatcctgtt tggcccatat tcagctgttc catctgttct tggccctgag
      541 ccggggcagg aactgcttac cacagatatc ctgtttggcc catattcagc tgttccatct
      601 gttcctgacc ttgatctgaa cttctctatt ctcagttatg tatttttcca tgccttgcaa
      661 aatggcgtta cttaagctag cttgccaaac ctacaggtgg ggtctttcat tccccccttt
      721 ttctggagac taaataaaat cttttatttt atcgatgcat ggggtcgtgc gctcctttcg
      781 gtcgggcgct gcgggtcgtg gggcgggcgt caggcaccgg gcttgcgggt catgcaccag
      841 gtgcgcggtc cttcgggcac ctcgacgtcg gcggtgacgg tgaagccgag ccgctcgtag
      901 aaggggaggt tgcggggcgc ggaggtctcc aggaaggcgg gcaccccggc gcgctcggcc
      961 gcctccactc cggggagcac gacggcgctg cccagaccct tgccctggtg gtcgggcgag
     1021 acgccgacgg tggccaggaa ccacgcgggc tccttgggcc ggtgcggcgc caggaggcct
     1081 tccatctgtt gctgcgcggc cagccgggaa ccgctcaact cggccatgcg cgggccgatc
     1141 tcggcgaaca ccgcccccgc ttcgacgctc tccggcgtgg tccagaccgc caccgcggcg
     1201 ccgtcgtccg cgacccacac cttgccgatg tcgagcccga cgcgcgtgag gaagagttct
     1261 tgcagctcgg tgacccgctc gatgtggcgg tccggatcga cggtgtggcg cgtggcgggg
     1321 tagtcggcga acgcggcggc gagggtgcgt acggccctgg ggacgtcgtc gcgggtggcg
     1381 aggcgcaccg tgggcttgta ctcggtcatg gtaagctttt tgcaaaagcc taggcctcca
     1441 aaaaagcctc ctcactactt ctggaatagc tcagaggccg aggcggcctc ggcctctgca
     1501 taaataaaaa aaattagtca gccatggggc ggagaatggg cggaactggg cggagttagg
     1561 ggcgggatgg gcggagttag gggcgggact atggttgctg actaattgag atgcatgctt
     1621 tgcatacttc tgcctgctgg ggagcctggg gactttccac acctggttgc tgactaattg
     1681 agatgcatgc tttgcatact tctgcctgct ggggagcctg gggactttcc acaccctaac
     1741 tgacacacat tccacagggt cgaccactgt gctggcgaat tcctacgtac caccacactg
     1801 ggatccccgg gcgagctcaa gacactgttt tcaatagaat ttcacgtttg cggtttggtt
     1861 ctctgtttca gacatgaact gcttttcaga tgaaaaacct ggggtggcag aggtgtgttt
     1921 gtgctgtgga agaacccagg gcagaaaaat aacagggagt aacaagaggt gcattctctg
     1981 gtcagacagc caagtgagga ggtagttatt ctgagaactt ctcaaataga gattattgta
     2041 ctaaactact gagagctggg gaagtagcag gagcttctct gtgagactga agacagcaac
     2101 aggaatactt ctcctccagg gaagtcagga ccagggtgtc agtgcagctt gaaagtggct
     2161 cttttctgac aaaactttaa aagtatcttg ttacatcacc acacatggca atcccataca
     2221 accctgagtc ccatcatcac tgctgttgag ttttttcatt cggtactggc gtatttctct
     2281 taccagtgtg taaaaagcat cttcaacacc ctgtctggtc ttggctgagg tttcaatgaa
     2341 tggaatcccg taactcttgg ccagttcgtg ggcttgtttt gtatcaactg tccttgttgg
     2401 caaatcacac ttgtttccca ctagcaccat aggtacatca tccgagtctt ttactcgctt
     2461 aatctgctcc ctgtagaggt taatatccgc aaatgacttg ctattattga tggcaaatac
     2521 acagaggaag ccttcgcctg tcctcatgta ttggtctctc atggcactgt actcttcttt
     2581 tccagctgta tccagtatgt ccaacaaaca ggtttcacca tctataacca cttgttttct
     2641 gtaagaatcc tctatggtgg gatcatattc atctacaaag tggttctgga ttagctggat
     2701 tgtcagtgcg cttttcccaa caccacctgc tccaaccacc accagtttgt actcagtcat
     2761 ttcacaccag caagaacctc aagctccact gcctctgctt tggacagatt taggaccaca
     2821 gccgggggaa ttagcttggg ctgcaggtcg actctagagg atccggccgg cgcctagaga
     2881 aggagtgagg gctggataaa gggaggatcg aggcggggtc gaacgaggag gttcaagggg
     2941 gagagacggg gcggatggag gaagaggagg cggaggctta gggtgtacaa agggcttgac
     3001 ccagggaggg gggtcaaaag ccaaggcttc ccaggtcacg atgtagggga cctggtctgg
     3061 gtgtccatgc gggccaggtg aaaagacctt gatcttaacc tgggtgatga ggtctcggtt
     3121 aaaggtgccg tctcgcggcc atccgacgtt aaaggttggc cattctgcag agcagaaggt
     3181 aacccaacgt ctcttcttga catctaccga ctggttgtga gcgatccgct cgacatcttt
     3241 ccagtgacct aaggtcaaac ttaagggagt ggtaacagtc tggccctaat tttcagacaa
     3301 atacagaaac acagtcagac agagacaaca cagaacgatg ctgcagcgct gcagcagaca
     3361 agacgcgcgg cttcggttcc aaaccgaaag caaaaattca gacggaggcg ggaactgttt
     3421 taggttctcg tctcctacca gaaccacata tcctgacggg gtcggattcc acatcgactc
     3481 ccttcctcag gtcgggccac aaaaacggcc cccaaagtcc ctgggacgtc tcccagggtt
     3541 gcggccgggt gttcagaact cgtcagttcc accacgggtc cgccagatac agagctagtt
     3601 agctaactag taccgacgca ggcgcataaa atcagtcata gacactagac aatcggacag
     3661 acacagataa gttgctggcc agcttgcctc ccggtggtgg gtcggtggtc cctgggcagg
     3721 ggtctcccga tcccggacga gcccccaaat gaaagacccc cgctgacggg tagtcaatca
     3781 ctcagaggag accctcccaa ggaacagcga gaccacaagt cggatgcaac tgcaagaggg
     3841 tttattggat acacgggtac ccgggcgact cagtcaatcg gaggactggc gccccgagtg
     3901 aggggttgtg ggctctttta ttgagctcgg ggagcagaag cgcgcgaaca gaagcgagaa
     3961 gcgaactgat tggttagttc aaataaggca cagggtcatt tcaggtcctt ggggcaccct
     4021 ggaaacatct gatggttctc tagaaactgc tgagggctgg accgcatctg gggaccatct
     4081 gttcttggcc ctgagccggg gcaggaactg cttaccacag atatcctgtt tggcccatat
     4141 tcagctgttc catctgttct tggccctgag ccggggcagg aactgcttac cacagatatc
     4201 ctgtttggcc catattcagg ctgcaggtgg cacttttcgg ggaaatgtgc gcggaacccc
     4261 tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg
     4321 ataaatgctt caataatatt gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc
     4381 ccttattccc ttttttgcgg cattttgcct tcctgttttt gctcacccag aaacgctggt
     4441 gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg ggttacatcg aactggatct
     4501 caacagcggt aagatccttg agagttttcg ccccgaagaa cgttttccaa tgatgagcac
     4561 ttttaaagtt ctgctatgtg gcgcggtatt atcccgtatt gacgccgggc aagagcaact
     4621 cggtcgccgc atacactatt ctcagaatga cttggttgag tactcaccag tcacagaaaa
     4681 gcatcttacg gatggcatga cagtaagaga attatgcagt gctgccataa ccatgagtga
     4741 taacactgcg gccaacttac ttctgacaac gatcggagga ccgaaggagc taaccgcttt
     4801 tttgcacaac atgggggatc atgtaactcg ccttgatcgt tgggaaccgg agctgaatga
     4861 agccatacca aacgacgagc gtgacaccac gatgcctgta gcaatggcaa caacgttgcg
     4921 caaactatta actggcgaac tacttactct agcttcccgg caacaattaa tagactggat
     4981 ggaggcggat aaagttgcag gaccacttct gcgctcggcc cttccggctg gctggtttat
     5041 tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt atcattgcag cactggggcc
     5101 agatggtaag ccctcccgta tcgtagttat ctacacgacg gggagtcagg caactatgga
     5161 tgaacgaaat agacagatcg ctgagatagg tgcctcactg attaagcatt ggtaactgtc
     5221 agaccaagtt tactcatata tactttagat tgatttgcgg ccgcaaaagg atctaggtga
     5281 agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag
     5341 cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa
     5401 tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag
     5461 agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg
     5521 ttcttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat
     5581 acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta
     5641 ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg
     5701 gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc
     5761 gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa
     5821 gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc
     5881 tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt
     5941 caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct
     6001 tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc
     6061 gtattaccgc ctttgagtga gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg
     6121 agtcagtgag cgaggaagcg gaag
//
