LOCUS       sequence-162883-       11699 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway cloning compatible binary vector for N-terminal fusion with 
            FLAG (CaMV35S promoter)..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11699)
  AUTHORS   Nakagawa T, Suzuki T, Murata S, Nakamura S, Hino T, Maeo K, Tabata 
            R, Kawai T, Tanaka K, Niwa Y, Watanabe Y, Nakamura K, Kimura T, 
            Ishiguro S
  TITLE     Improved Gateway binary vectors: high-performance vectors for 
            creation of fusion constructs in transgenic analysis of plants.
  JOURNAL   Biosci Biotechnol Biochem. 2007 Aug;71(8):2095-100. Epub 2007 Aug 7.
  PUBMED    17690442
REFERENCE   2  (bases 1 to 11699)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Biosci 
            Biotechnol Biochem."; date: "2007-08"; volume: "71(8)"; pages: 
            "2095-100. Epub 2007 Aug 7"
COMMENT     Sequence Label: pGWB412
FEATURES             Location/Qualifiers
     source          1..11699
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    54..78
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     primer_bind     266..288
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     280..297
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     281..297
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        812..1157
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     primer_bind     1100..1120
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
     CDS             1178..1201
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     protein_bind    1211..1335
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        join(1360..1365,1366..1383,1384..1390)
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     CDS             1444..2103
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(1510..1529)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     CDS             2445..2750
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     2629..2648
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
     protein_bind    complement(2791..2915)
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     terminator      2941..3193
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     primer_bind     complement(3227..3243)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3227..3243)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(3240..3262)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    3251..3267
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(3275..3281,3282..3299,3300..3305))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    3320..3341
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     terminator      complement(3375..3627)
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     CDS             complement(4017..4811)
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGDDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(4128..4147)
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     4738..4757
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     promoter        complement(4832..5015)
                     /function="NOS promoter"
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     promoter        complement(4868..5047)
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     misc_feature    5221..5245
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             5766..6557
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MGEAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      6803..7391
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7292..7311
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7545..7562
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    7577..7717
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(7643..7662)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     7803..7825
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     rep_origin      8061..8255
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     CDS             complement(8321..9394)
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     CDS             complement(9823..10452)
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
ORIGIN
        1 tttcacgccc ttttaaatat ccgattattc taataaacgc tcttttctct taggtttacc
       61 cgccaatata tcctgtcaaa cactgatagt ttaaactgaa ggcgggaaac gacaatctga
      121 tccaagctca agctgctcta gcattcgcca ttcaggctgc gcaactgttg ggaagggcga
      181 tcggtgcggg cctcttcgct attacgccag ctggcgaaag ggggatgtgc tgcaaggcga
      241 ttaagttggg taacgccagg gttttcccag tcacgacgtt gtaaaacgac ggccagtgcc
      301 aagcttgcat gcctgcaggt ccccagatta gccttttcaa tttcagaaag aatgctaacc
      361 cacagatggt tagagaggct tacgcagcag gtctcatcaa gacgatctac ccgagcaata
      421 atctccagga aatcaaatac cttcccaaga aggttaaaga tgcagtcaaa agattcagga
      481 ctaactgcat caagaacaca gagaaagata tatttctcaa gatcagaagt actattccag
      541 tatggacgat tcaaggcttg cttcacaaac caaggcaagt aatagagatt ggagtctcta
      601 aaaaggtagt tcccactgaa tcaaaggcca tggagtcaaa gattcaaata gaggacctaa
      661 cagaactcgc cgtaaagact ggcgaacagt tcatacagag tctcttacga ctcaatgaca
      721 agaagaaaat cttcgtcaac atggtggagc acgacacact tgtctactcc aaaaatatca
      781 aagatacagt ctcagaagac caaagggcaa ttgagacttt tcaacaaagg gtaatatccg
      841 gaaacctcct cggattccat tgcccagcta tctgtcactt tattgtgaag atagtggaaa
      901 aggaaggtgg ctcctacaaa tgccatcatt gcgataaagg aaaggccatc gttgaagatg
      961 cctctgccga cagtggtccc aaagatggac ccccacccac gaggagcatc gtggaaaaag
     1021 aagacgttcc aaccacgtct tcaaagcaag tggattgatg tgatatctcc actgacgtaa
     1081 gggatgacgc acaatcccac tatccttcgc aagacccttc ctctatataa ggaagttcat
     1141 ttcatttgga gagaacacgg gggactctag aatgagcgac tacaaggatg acgatgacaa
     1201 ggctgttatc acaagtttgt acaaaaaagc tgaacgagaa acgtaaaatg atataaatat
     1261 caatatatta aattagattt tgcataaaaa acagactaca taatactgta aaacacaaca
     1321 tatccagtca ctatggcggc cgcattaggc accccaggct ttacacttta tgcttccggc
     1381 tcgtataatg tgtggatttt gagttaggat ccgtcgagat tttcaggagc taaggaagct
     1441 aaaatggaga aaaaaatcac tggatatacc accgttgata tatcccaatg gcatcgtaaa
     1501 gaacattttg aggcatttca gtcagttgct caatgtacct ataaccagac cgttcagctg
     1561 gatattacgg cctttttaaa gaccgtaaag aaaaataagc acaagtttta tccggccttt
     1621 attcacattc ttgcccgcct gatgaatgct catccggaat tccgtatggc aatgaaagac
     1681 ggtgagctgg tgatatggga tagtgttcac ccttgttaca ccgttttcca tgagcaaact
     1741 gaaacgtttt catcgctctg gagtgaatac cacgacgatt tccggcagtt tctacacata
     1801 tattcgcaag atgtggcgtg ttacggtgaa aacctggcct atttccctaa agggtttatt
     1861 gagaatatgt ttttcgtctc agccaatccc tgggtgagtt tcaccagttt tgatttaaac
     1921 gtggccaata tggacaactt cttcgccccc gttttcacca tgggcaaata ttatacgcaa
     1981 ggcgacaagg tgctgatgcc gctggcgatt caggttcatc atgccgtctg tgatggcttc
     2041 catgtcggca gaatgcttaa tgaattacaa cagtactgcg atgagtggca gggcggggcg
     2101 taaacgcgtg gatccggctt actaaaagcc agataacagt atgcgtattt gcgcgctgat
     2161 ttttgcggta taagaatata tactgatatg tatacccgaa gtatgtcaaa aagaggtgtg
     2221 ctatgaagca gcgtattaca gtgacagttg acagcgacag ctatcagttg ctcaaggcat
     2281 atatgatgtc aatatctccg gtctggtaag cacaaccatg cagaatgaag cccgtcgtct
     2341 gcgtgccgaa cgctggaaag cggaaaatca ggaagggatg gctgaggtcg cccggtttat
     2401 tgaaatgaac ggctcttttg ctgacgagaa cagggactgg tgaaatgcag tttaaggttt
     2461 acacctataa aagagagagc cgttatcgtc tgtttgtgga tgtacagagt gatattattg
     2521 acacgcccgg gcgacggatg gtgatccccc tggccagtgc acgtctgctg tcagataaag
     2581 tctcccgtga actttacccg gtggtgcata tcggggatga aagctggcgc atgatgacca
     2641 ccgatatggc cagtgtgccg gtctccgtta tcggggaaga agtggctgat ctcagccacc
     2701 gcgaaaatga catcaaaaac gccattaacc tgatgttctg gggaatataa atgtcaggct
     2761 cccttataca cagccagtct gcaggtcgac catagtgact ggatatgttg tgttttacag
     2821 tattatgtag tctgtttttt atgcaaaatc taatttaata tattgatatt tatatcattt
     2881 tacgtttctc gttcagcttt cttgtacaaa gtggtgataa cctagagctc gaatttcccc
     2941 gatcgttcaa acatttggca ataaagtttc ttaagattga atcctgttgc cggtcttgcg
     3001 atgattatca tataatttct gttgaattac gttaagcatg taataattaa catgtaatgc
     3061 atgacgttat ttatgagatg ggtttttatg attagagtcc cgcaattata catttaatac
     3121 gcgatagaaa acaaaatata gcgcgcaaac taggataaat tatcgcgcgc ggtgtcatct
     3181 atgttactag atcgggaatt ggttccggaa ccaattcgta atcatggtca tagctgtttc
     3241 ctgtgtgaaa ttgttatccg ctcacaattc cacacaacat acgagccgga agcataaagt
     3301 gtaaagcctg gggtgcctaa tgagtgagct aactcacatt aggctgaatt aggcgcgcct
     3361 atttctgaat tcccgatcta gtaacataga tgacaccgcg cgcgataatt tatcctagtt
     3421 tgcgcgctat attttgtttt ctatcgcgta ttaaatgtat aattgcggga ctctaatcat
     3481 aaaaacccat ctcataaata acgtcatgca ttacatgtta attattacat gcttaacgta
     3541 attcaacaga aattatatga taatcatcgc aagaccggca acaggattca atcttaagaa
     3601 actttattgc caaatgtttg aacgatcggg gatcatccgg gtctgtggcg ggaactccac
     3661 gaaaatatcc gaacgcagca agatatcgcg gtgcatctcg gtcttgcctg ggcagtcgcc
     3721 gccgacgccg ttgatgtgga cgccgggccc gatcatattg tcgctcagga tcgtggcgtt
     3781 gtgcttgtcg gccgttgctg tcgtaatgat atcggcacct tcgaccgcct gttccgcaga
     3841 gatcccgtgg gcgaagaact ccagcatgag atccccgcgc tggaggatca tccagccggc
     3901 gtcccggaaa acgattccga agcccaacct ttcatagaag gcggcggtgg aatcgaaatc
     3961 tcgtgatggc aggttgggcg tcgcttggtc ggtcatttcg aaccccagag tcccgctcag
     4021 aagaactcgt caagaaggcg atagaaggcg atgcgctgcg aatcgggagc ggcgataccg
     4081 taaagcacga ggaagcggtc agcccattcg ccgccaagct cttcagcaat atcacgggta
     4141 gccaacgcta tgtcctgata gcggtccgcc acacccagcc ggccacagtc gatgaatcca
     4201 gaaaagcggc cattttccac catgatattc ggcaagcagg catcgccatg ggtcacgacg
     4261 agatcatcgc cgtcgggcat gcgcgccttg agcctggcga acagttcggc tggcgcgagc
     4321 ccctgatgct cttcgtccag atcatcctga tcgacaagac cggcttccat ccgagtacgt
     4381 gctcgctcga tgcgatgttt cgcttggtgg tcgaatgggc aggtagccgg atcaagcgta
     4441 tgcagccgcc gcattgcatc agccatgatg gatactttct cggcaggagc aaggtgagat
     4501 gacaggagat cctgccccgg cacttcgccc aatagcagcc agtcccttcc cgcttcagtg
     4561 acaacgtcga gcacagctgc gcaaggaacg cccgtcgtgg ccagccacga tagccgcgct
     4621 gcctcgtcct gcagttcatt cagggcaccg gacaggtcgg tcttgacaaa aagaaccggg
     4681 cgcccctgcg ctgacagccg gaacacggcg gcatcagagc agccgattgt ctgttgtgcc
     4741 cagtcatagc cgaatagcct ctccacccaa gcggccggag aacctgcgtg caatccatct
     4801 tgttcaatca tgcgaaacga tccagatccg gtgcagatta tttggattga gagtgaatat
     4861 gagactctaa ttggataccg aggggaattt atggaacgtc agtggagcat ttttgacaag
     4921 aaatatttgc tagctgatag tgaccttagg cgacttttga acgcgcaata atggtttctg
     4981 acgtatgtgc ttagctcatt aaactccaga aacccgcggc tgagtggctc cttcaacgtt
     5041 gcggttctgt cagttccaaa cgtaaaacgg cttgtcccgc gtcatcggcg ggggtcataa
     5101 cgtgactccc ttaattctcc gctcatgatg aattcagaaa taaattcagc ctaattcggc
     5161 gttaattcag tacattaaaa acgtccgcaa tgtgttatta agttgtctaa gcgtcaattt
     5221 gtttacacca caatatatcc tgccaccagc cagccaacag ctccccgacc ggcagctcgg
     5281 cacaaaatca ccactcgata caggcagccc atcagtccgg gacggcgtca gcgggagagc
     5341 cgttgtaagg cggcagactt tgctcatgtt accgatgcta ttcggaagaa cggcaactaa
     5401 gctgccgggt ttgaaacacg gatgatctcg cggagggtag catgttgatt gtaacgatga
     5461 cagagcgttg ctgcctgtga tcaattcggg cacgaaccca gtggacataa gcctgttcgg
     5521 ttcgtaagct gtaatgcaag tagcgtatgc gctcacgcaa ctggtccaga accttgaccg
     5581 aacgcagcgg tggtaacggc gcagtggcgg ttttcatggc ttgttatgac tgtttttttg
     5641 gggtacagtc tatgcctcgg gcatccaagc agcaagcgcg ttacgccgtg ggtcgatgtt
     5701 tgatgttatg gagcagcaac gatgttacgc agcagggcag tcgccctaaa acaaagttaa
     5761 acatcatggg ggaagcggtg atcgccgaag tatcgactca actatcagag gtagttggcg
     5821 tcatcgagcg ccatctcgaa ccgacgttgc tggccgtaca tttgtacggc tccgcagtgg
     5881 atggcggcct gaagccacac agtgatattg atttgctggt tacggtgacc gtaaggcttg
     5941 atgaaacaac gcggcgagct ttgatcaacg accttttgga aacttcggct tcccctggag
     6001 agagcgagat tctccgcgct gtagaagtca ccattgttgt gcacgacgac atcattccgt
     6061 ggcgttatcc agctaagcgc gaactgcaat ttggagaatg gcagcgcaat gacattcttg
     6121 caggtatctt cgagccagcc acgatcgaca ttgatctggc tatcttgctg acaaaagcaa
     6181 gagaacatag cgttgccttg gtaggtccag cggcggagga actctttgat ccggttcctg
     6241 aacaggatct atttgaggcg ctaaatgaaa ccttaacgct atggaactcg ccgcccgact
     6301 gggctggcga tgagcgaaat gtagtgctta cgttgtcccg catttggtac agcgcagtaa
     6361 ccggcaaaat cgcgccgaag gatgtcgctg ccgactgggc aatggagcgc ctgccggccc
     6421 agtatcagcc cgtcatactt gaagctagac aggcttatct tggacaagaa gaagatcgct
     6481 tggcctcgcg cgcagatcag ttggaagaat ttgtccacta cgtgaaaggc gagatcacca
     6541 aggtagtcgg caaataatgt ctagctagaa attcgttcaa gccgacgccg cttcgcggcg
     6601 cggcttaact caagcgttag atgcactaag cacataattg ctcacagcca aactatcagg
     6661 tcaagtctgc ttttattatt tttaagcgtg cataataagc cctacacaaa ttgggagata
     6721 tatcatgcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg
     6781 tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc
     6841 aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc
     6901 tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtc cttctagtgt
     6961 agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc
     7021 taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact
     7081 caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac
     7141 agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag
     7201 aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg
     7261 gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg
     7321 tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga
     7381 gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt
     7441 ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct
     7501 ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg
     7561 aggaagcgga agagcgcctg atgcggtatt ttctccttac gcatctgtgc ggtatttcac
     7621 accgcatatg gtgcactctc agtacaatct gctctgatgc cgcatagtta agccagtata
     7681 cactccgcta tcgctacgtg actgggtcat ggctgcgccc cgacacccgc caacacccgc
     7741 tgacgcgccc tgacgggctt gtctgctccc ggcatccgct tacagacaag ctgtgaccgt
     7801 ctccgggagc tgcatgtgtc agaggttttc accgtcatca ccgaaacgcg cgaggcaggg
     7861 tgccttgatg tgggcgccgg cggtcgagtg gcgacggcgc ggcttgtccg cgccctggta
     7921 gattgcctgg ccgtaggcca gccatttttg agcggccagc ggccgcgata ggccgacgcg
     7981 aagcggcggg gcgtagggag cgcagcgacc gaagggtagg cgctttttgc agctcttcgg
     8041 ctgtgcgctg gccagacagt tatgcacagg ccaggcgggt tttaagagtt ttaataagtt
     8101 ttaaagagtt ttaggcggaa aaatcgcctt ttttctcttt tatatcagtc acttacatgt
     8161 gtgaccggtt cccaatgtac ggctttgggt tcccaatgta cgggttccgg ttcccaatgt
     8221 acggctttgg gttcccaatg tacgtgctat ccacaggaaa gagacctttt cgaccttttt
     8281 cccctgctag ggcaatttgc cctagcatct gctccgtaca ttaggaaccg gcggatgctt
     8341 cgccctcgat caggttgcgg tagcgcatga ctaggatcgg gccagcctgc cccgcctcct
     8401 ccttcaaatc gtactccggc aggtcatttg acccgatcag cttgcgcacg gtgaaacaga
     8461 acttcttgaa ctctccggcg ctgccactgc gttcgtagat cgtcttgaac aaccatctgg
     8521 cttctgcctt gcctgcggcg cggcgtgcca ggcggtagag aaaacggccg atgccgggat
     8581 cgatcaaaaa gtaatcgggg tgaaccgtca gcacgtccgg gttcttgcct tctgtgatct
     8641 cgcggtacat ccaatcagct agctcgatct cgatgtactc cggccgcccg gtttcgctct
     8701 ttacgatctt gtagcggcta atcaaggctt caccctcgga taccgtcacc aggcggccgt
     8761 tcttggcctt cttcgtacgc tgcatggcaa cgtgcgtggt gtttaaccga atgcaggttt
     8821 ctaccaggtc gtctttctgc tttccgccat cggctcgccg gcagaacttg agtacgtccg
     8881 caacgtgtgg acggaacacg cggccgggct tgtctccctt cccttcccgg tatcggttca
     8941 tggattcggt tagatgggaa accgccatca gtaccaggtc gtaatcccac acactggcca
     9001 tgccggccgg ccctgcggaa acctctacgt gcccgtctgg aagctcgtag cggatcacct
     9061 cgccagctcg tcggtcacgc ttcgacagac ggaaaacggc cacgtccatg atgctgcgac
     9121 tatcgcgggt gcccacgtca tagagcatcg gaacgaaaaa atctggttgc tcgtcgccct
     9181 tgggcggctt cctaatcgac ggcgcaccgg ctgccggcgg ttgccgggat tctttgcgga
     9241 ttcgatcagc ggccgcttgc cacgattcac cggggcgtgc ttctgcctcg atgcgttgcc
     9301 gctgggcggc ctgcgcggcc ttcaacttct ccaccaggtc atcacccagc gccgcgccga
     9361 tttgtaccgg gccggatggt ttgcgaccgc tcacgccgat tcctcgggct tgggggttcc
     9421 agtgccattg cagggccggc agacaaccca gccgcttacg cctggccaac cgcccgttcc
     9481 tccacacatg gggcattcca cggcgtcggt gcctggttgt tcttgatttt ccatgccgcc
     9541 tcctttagcc gctaaaattc atctactcat ttattcattt gctcatttac tctggtagct
     9601 gcgcgatgta ttcagatagc agctcggtaa tggtcttgcc ttggcgtacc gcgtacatct
     9661 tcagcttggt gtgatcctcc gccggcaact gaaagttgac ccgcttcatg gctggcgtgt
     9721 ctgccaggct ggccaacgtt gcagccttgc tgctgcgtgc gctcggacgg ccggcactta
     9781 gcgtgtttgt gcttttgctc attttctctt tacctcatta actcaaatga gttttgattt
     9841 aatttcagcg gccagcgcct ggacctcgcg ggcagcgtcg ccctcgggtt ctgattcaag
     9901 aacggttgtg ccggcggcgg cagtgcctgg gtagctcacg cgctgcgtga tacgggactc
     9961 aagaatgggc agctcgtacc cggccagcgc ctcggcaacc tcaccgccga tgcgcgtgcc
    10021 tttgatcgcc cgcgacacga caaaggccgc ttgtagcctt ccatccgtga cctcaatgcg
    10081 ctgcttaacc agctccacca ggtcggcggt ggcccatatg tcgtaagggc ttggctgcac
    10141 cggaatcagc acgaagtcgg ctgccttgat cgcggacaca gccaagtccg ccgcctgggg
    10201 cgctccgtcg atcactacga agtcgcgccg gccgatggcc ttcacgtcgc ggtcaatcgt
    10261 cgggcggtcg atgccgacaa cggttagcgg ttgatcttcc cgcacggccg cccaatcgcg
    10321 ggcactgccc tggggatcgg aatcgactaa cagaacatcg gccccggcga gttgcagggc
    10381 gcgggctaga tgggttgcga tggtcgtctt gcctgacccg cctttctggt taagtacagc
    10441 gataaccttc atgcgttccc cttgcgtatt tgtttattta ctcatcgcat catatacgca
    10501 gcgaccgcat gacgcaagct gttttactca aatacacatc acctttttag acggcggcgc
    10561 tcggtttctt cagcggccaa gctggccggc caggccgcca gcttggcatc agacaaaccg
    10621 gccaggattt catgcagccg cacggttgag acgtgcgcgg gcggctcgaa cacgtacccg
    10681 gccgcgatca tctccgcctc gatctcttcg gtaatgaaaa acggttcgtc ctggccgtcc
    10741 tggtgcggtt tcatgcttgt tcctcttggc gttcattctc ggcggccgcc agggcgtcgg
    10801 cctcggtcaa tgcgtcctca cggaaggcac cgcgccgcct ggcctcggtg ggcgtcactt
    10861 cctcgctgcg ctcaagtgcg cggtacaggg tcgagcgatg cacgccaagc agtgcagccg
    10921 cctctttcac ggtgcggcct tcctggtcga tcagctcgcg ggcgtgcgcg atctgtgccg
    10981 gggtgagggt agggcggggg ccaaacttca cgcctcgggc cttggcggcc tcgcgcccgc
    11041 tccgggtgcg gtcgatgatt agggaacgct cgaactcggc aatgccggcg aacacggtca
    11101 acaccatgcg gccggccggc gtggtggtgt cggcccacgg ctctgccagg ctacgcaggc
    11161 ccgcgccggc ctcctggatg cgctcggcaa tgtccagtag gtcgcgggtg ctgcgggcca
    11221 ggcggtctag cctggtcact gtcacaacgt cgccagggcg taggtggtca agcatcctgg
    11281 ccagctccgg gcggtcgcgc ctggtgccgg tgatcttctc ggaaaacagc ttggtgcagc
    11341 cggccgcgtg cagttcggcc cgttggttgg tcaagtcctg gtcgtcggtg ctgacgcggg
    11401 catagcccag caggccagcg gcggcgctct tgttcatggc gtaatgtctc cggttctagt
    11461 cgcaagtatt ctactttatg cgactaaaac acgcgacaag aaaacgccag gaaaagggca
    11521 gggcggcagc ctgtcgcgta acttaggact tgtgcgacat gtcgttttca gaagacggct
    11581 gcactgaacg tcagaagccg actgcactat agcagcggag gggttggatc aaagtacttt
    11641 gatcccgagg ggaaccctgt ggttggcatg cacatacaaa tggacgaacg gataaacct
//
