LOCUS       sequence-162895-         656 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Gateway cloning compatible binary vector for N-terminal fusion with 
            3xHA (CaMV35S promoter)..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 656)
  AUTHORS   Nakagawa T, Suzuki T, Murata S, Nakamura S, Hino T, Maeo K, Tabata 
            R, Kawai T, Tanaka K, Niwa Y, Watanabe Y, Nakamura K, Kimura T, 
            Ishiguro S
  TITLE     Improved Gateway binary vectors: high-performance vectors for 
            creation of fusion constructs in transgenic analysis of plants.
  JOURNAL   Biosci Biotechnol Biochem. 2007 Aug;71(8):2095-100. Epub 2007 Aug 7.
  PUBMED    17690442
REFERENCE   2  (bases 1 to 656)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Biosci 
            Biotechnol Biochem."; date: "2007-08"; volume: "71(8)"; pages: 
            "2095-100. Epub 2007 Aug 7"
COMMENT     Sequence Label: pGWB415
FEATURES             Location/Qualifiers
     source          1..656
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(join(82..88,89..106,107..112))
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     protein_bind    complement(149..273)
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             complement(295..384)
                     /codon_start=1
                     /product="three tandem HA epitope tags"
                     /label=3xHA
                     /translation="YPYDVPDYAGYPYDVPDYAGSYPYDVPDYA"
     promoter        complement(425..471)
                     /label=minimal CaMV 35S promoter
                     /note="minimal 35S promoter from cauliflower mosaic virus"
     primer_bind     complement(460..480)
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
ORIGIN
        1 tggtatatcc agtgattttt ttctccattt tagcttcctt agctcctgaa aatctcgacg
       61 gatcctaact caaaatccac acattatacg agccggaagc ataaagtgta aagcctgggg
      121 tgcctaatgc ggcccctcga ggggccgcca tagtgactgg atatgttgtg ttttacagta
      181 ttatgtagtc tgttttttat gcaaaatcta atttaatata ttgatattta tatcatttta
      241 cgtttctcgt tcagcttttt tgtacaaact tgtgataaca gcgctgcact gagcagcgta
      301 atctggaacg tcatatggat aggatcctgc atagtccggg acgtcatagg gatagcccgc
      361 atagtcagga acatcgtatg ggtaaaagat gttaattaac ccgctcattc tagagtcccc
      421 cgtgttctct ccaaatgaaa tgaacttcct tatatagagg aagggtcttg cgaaggatag
      481 tgggattgtg cgtcatccct tacgtcagtg gagatatcac atcaatccac ttgctttgaa
      541 gacgtggttg gaacgtcttc tttttccacg atgctcctcg tgggtggggg tccatctttg
      601 ggaccactgt cggcagaggc atcttcaacg atggcctttc ctttatcgca atgatg
//
