LOCUS       sequence-162900-         900 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  YFP-CFP positive FRET control; his selection.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 900)
  AUTHORS   Hailey DW, Davis TN, Muller EG
  TITLE     Fluorescence resonance energy transfer using color variants of green
            fluorescent protein.
  JOURNAL   Methods Enzymol. 2002;351:34-49.
  PUBMED    12073355
REFERENCE   2  (bases 1 to 900)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Methods 
            Enzymol."; date: "2002"; volume: "351"; pages: "34-49"
COMMENT     Sequence Label: pDH18
FEATURES             Location/Qualifiers
     source          1..900
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             37..753
                     /codon_start=1
                     /product="S65T variant of Aequorea victoria green 
                     fluorescent protein (Heim et al., 1995)"
                     /label=GFP (S65T)
                     /note="excitable with blue light"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTFGYGLKCFARYPDHMKRHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALSKDPNEKRDHMVLL
                     EFVTAAGITHGMDELYK"
     CDS             37..753
                     /codon_start=1
                     /product="Aequorea victoria green fluorescent protein"
                     /label=GFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTFGYGLKCFARYPDHMKRHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALSKDPNEKRDHMVLL
                     EFVTAAGITHGMDELYK"
     primer_bind     693..713
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
ORIGIN
        1 tacgctgcag gtcgacggat ccccgggtta attaacatgg tgagtaaagg agaagaactt
       61 ttcactggag ttgtcccaat tcttgttgaa ttagatggtg atgttaatgg gcacaaattt
      121 tctgtcagtg gagagggtga aggtgatgca acatacggaa aacttaccct taaatttatt
      181 tgcactactg gaaaactacc tgttccatgg ccaacacttg tcactacttt cggttatggt
      241 ctaaaatgct ttgctagata cccagatcat atgaaacggc atgacttttt caagagtgcc
      301 atgcccgaag gttatgtaca ggaaagaact atatttttca aagatgacgg gaactacaag
      361 acacgtgctg aagtcaagtt tgaaggtgat acccttgtta atagaatcga gttaaaaggt
      421 attgatttta aagaagatgg aaacattctt ggacacaaat tggaatacaa ctataactca
      481 cacaatgtat acatcatggc agacaaacaa aagaatggaa tcaaagttaa cttcaaaatt
      541 agacacaaca ttgaagatgg aagcgttcaa ctagcagacc attatcaaca aaatactcca
      601 attggcgatg gccctgtcct tttaccagac aaccattacc tgtcctatca atctgccctt
      661 tcgaaagatc ccaacgaaaa gagagaccac atggtccttc ttgagtttgt aacagctgct
      721 gggattacac atggcatgga tgaactatac aaaggcgcca gtaaaggaga agaacttttc
      781 actggagttg tcccaattct tgttgaatta gatggtgatg ttaatgggca caaattttct
      841 gtcagtggag agggtgaagg tgatgcaaca tacggaaaac ttacccttaa atttatttgc
//
