LOCUS       sequence-162904-        4176 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway Donor vector containing NFIL3, used as an over expression 
            control for gene expression analysis. Part of the Target Accelerator
            Plasmid Collection..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4176)
  AUTHORS   Berger AH, Brooks AN, Wu X, Shrestha Y, Chouinard C, Piccioni F, 
            Bagul M, Kamburov A, Imielinski M, Hogstrom L, Zhu C, Yang X, Pantel
            S, Sakai R, Watson J, Kaplan N, Campbell JD, Singh S, Root DE, 
            Narayan R, Natoli T, Lahr DL, Tirosh I, Tamayo P, Getz G, Wong B, 
            Doench J, Subramanian A, Golub TR, Meyerson M, Boehm JS
  TITLE     High-throughput Phenotyping of Lung Cancer Somatic Mutations.
  JOURNAL   Cancer Cell. 2016 Aug 8;30(2):214-28. doi: 
            10.1016/j.ccell.2016.06.022. Epub 2016 Jul 28.
  PUBMED    27478040
REFERENCE   2  (bases 1 to 4176)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.ccell.2016.06.022"; journalName: "Cancer Cell"; date: 
            "2016-08-8- 8"; volume: "30"; issue: "2"; pages: "214-28"
COMMENT     Sequence Label: pDONR223_NFIL3_WT_V5
FEATURES             Location/Qualifiers
     source          1..4176
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(143..160)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(314..902)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(394..413)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(995..1786)
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     primer_bind     2217..2233
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     2217..2233
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     2238..2257
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        2238..2256
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    2274..2373
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(3760..3859)
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(3875..3892)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(3875..3891)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3884..3906)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     terminator      3955..4041
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      4133..4160
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
ORIGIN
        1 cttcctggct agcggtacgc gtattaattg cgttgcgctc actgcccgct ttccagtcgg
       61 gaaacctgtc gtgccagctg cattaatgaa tcggccaacg cgcggggaga ggcggtttgc
      121 gtattgggcg ctcttccgct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc
      181 ggcgagcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata
      241 acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg
      301 cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct
      361 caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa
      421 gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc
      481 tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt
      541 aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg
      601 ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg
      661 cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct
      721 tgaagtggtg gcctaactac ggctacacta gaagaacagt atttggtatc tgcgctctgc
      781 tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg
      841 ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc
      901 aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgac gcgtaactca
      961 cgttaaggga ttttggtcat gggtggctcg agggttattt gccgactacc ttggtgatct
     1021 cgcctttcac gtagtggaca aattcttcca actgatctgc gcgcgaggcc aagcgatctt
     1081 cttcttgtcc aagataagcc tgtctagctt caagtatgac gggctgatac tgggccggca
     1141 ggcgctccat tgcccagtcg gcagcgacat ccttcggcgc gattttgccg gttactgcgc
     1201 tgtaccaaat gcgggacaac gtaagcacta catttcgctc atcgccagcc cagtcgggcg
     1261 gcgagttcca tagcgttaag gtttcattta gcgcctcaaa tagatcctgt tcaggaaccg
     1321 gatcaaagag ttcctccgcc gctggaccta ccaaggcaac gctatgttct cttgcttttg
     1381 tcagcaagat agccagatca atgtcgatcg tggctggctc gaagatacct gcaagaatgt
     1441 cattgcgctg ccattctcca aattgcagtt cgcgcttagc tggataacgc cacggaatga
     1501 tgtcgtcgtg cacaacaatg gtgacttcta cagcgcggag aatctcgctc tctccagggg
     1561 aagccgaagt ttccaaaagg tcgttgatca aagctcgccg cgttgtttca tcaagcctta
     1621 cggtcaccgt aaccagcaaa tcaatatcac tgtgtggctt caggccgcca tccactgcgg
     1681 agccgtacaa atgtacggcc agcaacgtcg gttcgagatg gcgctcgatg acgccaacta
     1741 cctctgatag ttgagtcgat acttcggcga tcaccgcttc cctcataatg tttaactttg
     1801 ttttagggcg actgccctgc tgcgtaacat cgttgctgct ccataacatc aaacatcgac
     1861 ccacggcgta acgcgcttgc tgcttggatg cccgaggcat agactgtacc ccaaaaaaac
     1921 agtcataaca agccatgaaa accgccactg cgccgttacc accgctgcgt tcggtcaagg
     1981 ttctggacca gttgcgtgag cgcatacgct acttgcatta cagcttacga accgaacagg
     2041 cttatgtcca ctgggttcgt gccttcatcc gtttccacgg tgtgcgtcac ccggcaacct
     2101 tgggtagcag cgaagtcgag gcatttctgt cctggctggt ctagaggagg catgatgata
     2161 tatttttatc ttgtgcaatg taacatcaga gattttgaga cacgggccag agctgccagg
     2221 aaacagctat gaccatgtaa tacgactcac tataggggat atcagctgga tggcaaataa
     2281 tgattttatt ttgactgata gtgacctgtt cgttgcaaca aattgataag caatgctttc
     2341 ttataatgcc aactttgtac aagaaagttg ggtacccaga gtctgaagca gagattggtt
     2401 gtgtggctat aagtctcttg agtgagacaa cctctgcaga taagtttgct atcccctgct
     2461 tcaaatacaa gttctctggg tcagaaactt tgtagccact gtctttcatt tcaacaactc
     2521 cagttttgaa actattctga gttttgccac tcagttcttt ttgatgccag tgctccgatt
     2581 tgagagacca atcttgaatg ttagtcactt gcactgagaa aggagtaaga gaagaatgta
     2641 ccatacttgg ggcactatgc ttttcgagtt cgaaatgtct tttagatgtc atgtcaatag
     2701 gtgaggaaag tttttgcgtg gcctcaaatt cattatcaaa ggcttctact ttgatctgca
     2761 tggctttggc tttgatccgg agcttgtgtg gcaaggcaga ggaattcact tctggaactt
     2821 taaccacagt tgcatgcaca tgcttgagtt caactggaga atggatgggg cccttgggga
     2881 cctgttgctc gtcttctcca tcagatgact ttcctaccac accatcatca gtttccgacg
     2941 ttctcgggga gttgctggag gatcggttga cttgcagtag tgggggagag tgtgagtacc
     3001 cagagaaaga attccccata tagttttgat agatggacgc tgtgtaagag cctcggtcat
     3061 ctcttggctc ccttgtgtag ctctctaatt ccatcggctc ttgcttgata atctggaact
     3121 tgttttcagg acttctgcag cttccctgca cagagctctc ctgcgtgtgt tctactgagg
     3181 acacttctga aacatcggac agcgagcttt gtggagagtg tttaatgaca gaaatacaac
     3241 tacttgacac catcgagggt tcgtgctcgt ccacaaatga actcacattg gatttggaag
     3301 tctggtaatc ttgaaagtac acagctgtag aattactgag tttctgaatc tcttgagcat
     3361 atgctgtgga gctaattaaa ccaaacttta attttagtga aagcagctca gcttttaaag
     3421 tggcgttttc ttctcccagt gcaattagtt tgttctctaa aaccaggtca ttcagtcgac
     3481 gcttctcacg agatcttttg gcagcttcat tatttttccg ccttttttcc caatacatag
     3541 catctttctt ttcatcagga atgaattccc gtttcctccg acatgcagaa gatttgttct
     3601 tccccacact tccttcactg agaagcagct cctcacctgt tgtggagtct tctgacactt
     3661 ccgttaaagc agaattaagg accatcatct tgtccacatt gctactggca tcaagagacg
     3721 cctgctcctt tttgacggtc tgcatttttc tcagctgcat gccaactttt ttgtacaaag
     3781 ttggcattat aaaaaagcat tgctcatcaa tttgttgcaa cgaacaggtc actatcagtc
     3841 aaaataaaat cattatttgg ggcccgagct taagactggc cgtcgtttta caacgtcgtg
     3901 actgggaaaa catccatgct agcgttaacg cgagagtagg gaactgccag gcatcaaata
     3961 aaacgaaagg ctcagtcgga agactgggcc tttcgtttta tctgttgttt gtcggtgaac
     4021 gctctcctga gtaggacaaa tccgccggga gcggatttga acgttgtgaa gcaacggccc
     4081 ggagggtggc gggcaggacg cccgccataa actgccaggc atcaaactaa gcagaaggcc
     4141 atcctgacgg atggcctttt tgcgtttcta caaact
//
