LOCUS       sequence-162907-         864 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 864)
  AUTHORS   Jensen MA, True HL, Chernoff YO, Lindquist S
  TITLE     Molecular population genetics and evolution of a prion-like protein 
            in Saccharomyces cerevisiae.
  JOURNAL   Genetics 2001 Oct;159(2):527-35.
  PUBMED    11606530
REFERENCE   2  (bases 1 to 864)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Genetics"; 
            date: "2001-10"; volume: "159(2)"; pages: "527-35"
COMMENT     Sequence Label: mCNMG
FEATURES             Location/Qualifiers
     source          1..864
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     2..21
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        2..20
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             complement(38..754)
                     /codon_start=1
                     /product="S65T variant of Aequorea victoria green 
                     fluorescent protein (Heim et al., 1995)"
                     /label=GFP (S65T)
                     /note="excitable with blue light"
                     /translation="MASKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTFTYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     ANFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITHGMDELYK"
     primer_bind     complement(78..98)
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
     primer_bind     693..721
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
ORIGIN
        1 gtaatacgac tcactatagg gcgaattgga gctctcattt gtatagttca tccatgccat
       61 gtgtaatccc agcagctgtt acaaactcaa gaaggaccat gtggtctctc ttttcgttgg
      121 gatctttcga aagggcagat tgtgtggaca ggtaatggtt gtctggtaaa aggacagggc
      181 catcgccaat tggagtattt tgttgataat ggtctgctag ttgaacgctt ccatcttcaa
      241 tgttgtgtct aattttgaag ttagctttga ttccattctt ttgtttgtct gccatgatgt
      301 atacattgtg tgagttatag ttgtattcca atttgtgccc aagaatgttt ccatcttctt
      361 taaaatcaat accttttaac tcgattctat taacaagggt atcaccttca aacttgactt
      421 cagcacgtgt cttgtagttc ccgtcatctt tgaaaaatat agttctttcc tgtacataac
      481 cttcgggcat ggcactcttg aaaaagtcat gccgtttcat atgatccggg tatcttgaaa
      541 agcactgaac accataagtg aaagtagtga caagtgttgg ccatggaaca ggtagttttc
      601 cagtagtgca aataaattta agggtaagtt ttccgtatgt tgcatcacct tcaccctctc
      661 cactgacaga aaatttgtgc ccattaacat caccatctaa ttcaacaaga attgggacaa
      721 ctccagtgaa gagttcttct cctttgctag ccatccgcgg atcgttaaca acttcgtcat
      781 ccacttcttc ttcctgttcc ttgatcaagg catcagcact ggtaacattg gcattgttgg
      841 tattatgtgt tgattcagag attt
//
