LOCUS       sequence-162909-         862 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Gateway cloning compatible binary vector for N-terminal fusion with 
            G3GFP (CaMV35S promoter)..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 862)
  AUTHORS   Nakagawa T, Suzuki T, Murata S, Nakamura S, Hino T, Maeo K, Tabata 
            R, Kawai T, Tanaka K, Niwa Y, Watanabe Y, Nakamura K, Kimura T, 
            Ishiguro S
  TITLE     Improved Gateway binary vectors: high-performance vectors for 
            creation of fusion constructs in transgenic analysis of plants.
  JOURNAL   Biosci Biotechnol Biochem. 2007 Aug;71(8):2095-100. Epub 2007 Aug 7.
  PUBMED    17690442
REFERENCE   2  (bases 1 to 862)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Biosci 
            Biotechnol Biochem."; date: "2007-08"; volume: "71(8)"; pages: 
            "2095-100. Epub 2007 Aug 7"
COMMENT     Sequence Label: pGWB552
FEATURES             Location/Qualifiers
     source          1..862
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(51..79)
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
     primer_bind     674..694
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
     protein_bind    744..768
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 cgggggactc tagaatgagc atgagtaaag gagaagaact tttcactgga gttgtcccaa
       61 ttcttgttga attagatggt gatgttaatg ggcacaaatt ttctgtcagt ggagagggtg
      121 aaggtgatgc aacatacgga aaacttaccc ttaaatttat ttgcactact ggaaaactac
      181 ctgttccatg gccaacactt gttactactt tcgcttacgg tgtgcagtgc ttctcaagat
      241 acccagatca tatgaagcgg cacgacttct tcaagagcgc catgcctgag ggatacgtgc
      301 aggagaggac catcttcttc aaggacgacg ggaactacaa gacacgtgct gaagtcaagt
      361 ttgagggaga caccctcgtc aacaggatcg agcttaaggg aatcgatttc aaggaggacg
      421 gaaacatcct cggccacaag ttggaataca acttcaactc ccacaacgtg tacatcatgg
      481 cagacaaaca aaagaatgga atcaaagtta acttcaaaat tagacacaac attgaagatg
      541 gaagcgttca actagcagac cattatcaac aaaatactcc aattggcgat ggccctgtcc
      601 ttttaccaga caaccattac ctgtccacac aatctgccct ttcgaaagat cccaacgaaa
      661 agagagacca catggtcctt cttgagtttg taacagctgc tgggattaca catggcatgg
      721 atgaactata caaagctgtt atcacaagtt tgtacaaaaa agctgaacga gaaacgtaaa
      781 atgatataaa tatcaatata ttaaattaga ttttgcataa aaaacagact acataatact
      841 gtaaaacaca acatatccag tc
//
