LOCUS       sequence-162911-        3558 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway Donor vector containing ETFB, used as an over expression 
            control for gene expression analysis. Part of the Target Accelerator
            Plasmid Collection..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3558)
  AUTHORS   Berger AH, Brooks AN, Wu X, Shrestha Y, Chouinard C, Piccioni F, 
            Bagul M, Kamburov A, Imielinski M, Hogstrom L, Zhu C, Yang X, Pantel
            S, Sakai R, Watson J, Kaplan N, Campbell JD, Singh S, Root DE, 
            Narayan R, Natoli T, Lahr DL, Tirosh I, Tamayo P, Getz G, Wong B, 
            Doench J, Subramanian A, Golub TR, Meyerson M, Boehm JS
  TITLE     High-throughput Phenotyping of Lung Cancer Somatic Mutations.
  JOURNAL   Cancer Cell. 2016 Aug 8;30(2):214-28. doi: 
            10.1016/j.ccell.2016.06.022. Epub 2016 Jul 28.
  PUBMED    27478040
REFERENCE   2  (bases 1 to 3558)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.ccell.2016.06.022"; journalName: "Cancer Cell"; date: 
            "2016-08-8- 8"; volume: "30"; issue: "2"; pages: "214-28"
COMMENT     Sequence Label: pDONR223_ETFB_WT_V5
FEATURES             Location/Qualifiers
     source          1..3558
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    complement(238..337)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(354..373)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(355..373)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(378..394)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(378..394)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             825..1616
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      1709..2297
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2198..2217
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     2451..2468
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(2627..2654)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(2746..2832)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     2881..2903
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     2895..2912
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     2896..2912
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    2928..3027
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
ORIGIN
        1 tgccagctgt ggtgacagct gacctgaggc tcaacgagcc ccgctacgcc acgctgccca
       61 acatcatgaa agccaagaag aagaagatcg aggtgatcaa gcctggggac ctgggtgtgg
      121 acctgacctc caagctctct gtgatcagtg tggaggaccc gccccagcgc acggccggcg
      181 tcaaggtgga gaccactgag gacctggtgg ccaagctgaa ggagattggg cggattttgc
      241 caactttctt gtacaaagtt ggcattataa gaaagcattg cttatcaatt tgttgcaacg
      301 aacaggtcac tatcagtcaa aataaaatca ttatttgcca tccagctgat atcccctata
      361 gtgagtcgta ttacatggtc atagctgttt cctggcagct ctggcccgtg tctcaaaatc
      421 tctgatgtta cattgcacaa gataaaaata tatcatcatg cctcctctag accagccagg
      481 acagaaatgc ctcgacttcg ctgctaccca aggttgccgg gtgacgcaca ccgtggaaac
      541 ggatgaaggc acgaacccag tggacataag cctgttcggt tcgtaagctg taatgcaagt
      601 agcgtatgcg ctcacgcaac tggtccagaa ccttgaccga acgcagcggt ggtaacggcg
      661 cagtggcggt tttcatggct tgttatgact gtttttttgg ggtacagtct atgcctcggg
      721 catccaagca gcaagcgcgt tacgccgtgg gtcgatgttt gatgttatgg agcagcaacg
      781 atgttacgca gcagggcagt cgccctaaaa caaagttaaa cattatgagg gaagcggtga
      841 tcgccgaagt atcgactcaa ctatcagagg tagttggcgt catcgagcgc catctcgaac
      901 cgacgttgct ggccgtacat ttgtacggct ccgcagtgga tggcggcctg aagccacaca
      961 gtgatattga tttgctggtt acggtgaccg taaggcttga tgaaacaacg cggcgagctt
     1021 tgatcaacga ccttttggaa acttcggctt cccctggaga gagcgagatt ctccgcgctg
     1081 tagaagtcac cattgttgtg cacgacgaca tcattccgtg gcgttatcca gctaagcgcg
     1141 aactgcaatt tggagaatgg cagcgcaatg acattcttgc aggtatcttc gagccagcca
     1201 cgatcgacat tgatctggct atcttgctga caaaagcaag agaacatagc gttgccttgg
     1261 taggtccagc ggcggaggaa ctctttgatc cggttcctga acaggatcta tttgaggcgc
     1321 taaatgaaac cttaacgcta tggaactcgc cgcccgactg ggctggcgat gagcgaaatg
     1381 tagtgcttac gttgtcccgc atttggtaca gcgcagtaac cggcaaaatc gcgccgaagg
     1441 atgtcgctgc cgactgggca atggagcgcc tgccggccca gtatcagccc gtcatacttg
     1501 aagctagaca ggcttatctt ggacaagaag aagatcgctt ggcctcgcgc gcagatcagt
     1561 tggaagaatt tgtccactac gtgaaaggcg agatcaccaa ggtagtcggc aaataaccct
     1621 cgagccaccc atgaccaaaa tcccttaacg tgagttacgc gtcgttccac tgagcgtcag
     1681 accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct
     1741 gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac
     1801 caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat actgttcttc
     1861 tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg
     1921 ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt
     1981 tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt
     2041 gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc
     2101 tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca
     2161 gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata
     2221 gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg
     2281 ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct
     2341 ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta
     2401 ccgcctttga gtgagctgat accgctcgcc gcagccgaac gaccgagcgc agcgagtcag
     2461 tgagcgagga agcggaagag cgcccaatac gcaaaccgcc tctccccgcg cgttggccga
     2521 ttcattaatg cagctggcac gacaggtttc ccgactggaa agcgggcagt gagcgcaacg
     2581 caattaatac gcgtaccgct agccaggaag agtttgtaga aacgcaaaaa ggccatccgt
     2641 caggatggcc ttctgcttag tttgatgcct ggcagtttat ggcgggcgtc ctgcccgcca
     2701 ccctccgggc cgttgcttca caacgttcaa atccgctccc ggcggatttg tcctactcag
     2761 gagagcgttc accgacaaac aacagataaa acgaaaggcc cagtcttccg actgagcctt
     2821 tcgttttatt tgatgcctgg cagttcccta ctctcgcgtt aacgctagca tggatgtttt
     2881 cccagtcacg acgttgtaaa acgacggcca gtcttaagct cgggccccaa ataatgattt
     2941 tattttgact gatagtgacc tgttcgttgc aacaaattga tgagcaatgc ttttttataa
     3001 tgccaacttt gtacaaaaaa gttggcacca tggcggagct gcgcgtgctc gtagctgtca
     3061 agagggtcat cgactacgcc gtgaagatcc gagtgaagcc tgacaggacc ggtgtggtca
     3121 cggatggtgt gaagcactcc atgaacccct tctgtgagat cgcggtggag gaggctgtgc
     3181 ggctcaagga gaagaagctg gtgaaggagg tcatcgccgt cagctgtggg cctgcacagt
     3241 gccaggagac gattcgtacc gccctggcca tgggtgcaga ccgaggtatc cacgtggagg
     3301 tgcccccagc agaagcagaa cgcttgggtc ccctgcaggt ggctcgggtc ctggccaagc
     3361 tggcagagaa ggagaaggtg gacctggtgc tgctgggcaa acaggccatc gatgatgact
     3421 gtaaccagac agggcagatg acagctggat ttcttgactg gccacagggc acattcgcct
     3481 cccaggtgac gctggagggg gacaagttga aagtggagcg ggagatcgat gggggcctgg
     3541 agaccctgcg cctgaagc
//
