LOCUS       sequence-162912-        3411 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway Donor vector containing RALA, used as an over expression 
            control for gene expression analysis. Part of the Target Accelerator
            Plasmid Collection..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3411)
  AUTHORS   Berger AH, Brooks AN, Wu X, Shrestha Y, Chouinard C, Piccioni F, 
            Bagul M, Kamburov A, Imielinski M, Hogstrom L, Zhu C, Yang X, Pantel
            S, Sakai R, Watson J, Kaplan N, Campbell JD, Singh S, Root DE, 
            Narayan R, Natoli T, Lahr DL, Tirosh I, Tamayo P, Getz G, Wong B, 
            Doench J, Subramanian A, Golub TR, Meyerson M, Boehm JS
  TITLE     High-throughput Phenotyping of Lung Cancer Somatic Mutations.
  JOURNAL   Cancer Cell. 2016 Aug 8;30(2):214-28. doi: 
            10.1016/j.ccell.2016.06.022. Epub 2016 Jul 28.
  PUBMED    27478040
REFERENCE   2  (bases 1 to 3411)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.ccell.2016.06.022"; journalName: "Cancer Cell"; date: 
            "2016-08-8- 8"; volume: "30"; issue: "2"; pages: "214-28"
COMMENT     Sequence Label: pDONR223_RALA_WT_V5
FEATURES             Location/Qualifiers
     source          1..3411
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(104..123)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(105..123)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(128..144)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(128..144)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             575..1366
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      1459..2047
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1948..1967
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     2201..2218
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(2377..2404)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(2496..2582)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     2631..2653
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     2645..2662
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     2646..2662
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    2678..2777
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(join(3399..3411,1..87))
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
ORIGIN
        1 gtacaaagtt ggcattataa gaaagcattg cttatcaatt tgttgcaacg aacaggtcac
       61 tatcagtcaa aataaaatca ttatttgcca tccagctgat atcccctata gtgagtcgta
      121 ttacatggtc atagctgttt cctggcagct ctggcccgtg tctcaaaatc tctgatgtta
      181 cattgcacaa gataaaaata tatcatcatg cctcctctag accagccagg acagaaatgc
      241 ctcgacttcg ctgctaccca aggttgccgg gtgacgcaca ccgtggaaac ggatgaaggc
      301 acgaacccag tggacataag cctgttcggt tcgtaagctg taatgcaagt agcgtatgcg
      361 ctcacgcaac tggtccagaa ccttgaccga acgcagcggt ggtaacggcg cagtggcggt
      421 tttcatggct tgttatgact gtttttttgg ggtacagtct atgcctcggg catccaagca
      481 gcaagcgcgt tacgccgtgg gtcgatgttt gatgttatgg agcagcaacg atgttacgca
      541 gcagggcagt cgccctaaaa caaagttaaa cattatgagg gaagcggtga tcgccgaagt
      601 atcgactcaa ctatcagagg tagttggcgt catcgagcgc catctcgaac cgacgttgct
      661 ggccgtacat ttgtacggct ccgcagtgga tggcggcctg aagccacaca gtgatattga
      721 tttgctggtt acggtgaccg taaggcttga tgaaacaacg cggcgagctt tgatcaacga
      781 ccttttggaa acttcggctt cccctggaga gagcgagatt ctccgcgctg tagaagtcac
      841 cattgttgtg cacgacgaca tcattccgtg gcgttatcca gctaagcgcg aactgcaatt
      901 tggagaatgg cagcgcaatg acattcttgc aggtatcttc gagccagcca cgatcgacat
      961 tgatctggct atcttgctga caaaagcaag agaacatagc gttgccttgg taggtccagc
     1021 ggcggaggaa ctctttgatc cggttcctga acaggatcta tttgaggcgc taaatgaaac
     1081 cttaacgcta tggaactcgc cgcccgactg ggctggcgat gagcgaaatg tagtgcttac
     1141 gttgtcccgc atttggtaca gcgcagtaac cggcaaaatc gcgccgaagg atgtcgctgc
     1201 cgactgggca atggagcgcc tgccggccca gtatcagccc gtcatacttg aagctagaca
     1261 ggcttatctt ggacaagaag aagatcgctt ggcctcgcgc gcagatcagt tggaagaatt
     1321 tgtccactac gtgaaaggcg agatcaccaa ggtagtcggc aaataaccct cgagccaccc
     1381 atgaccaaaa tcccttaacg tgagttacgc gtcgttccac tgagcgtcag accccgtaga
     1441 aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac
     1501 aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt
     1561 tccgaaggta actggcttca gcagagcgca gataccaaat actgttcttc tagtgtagcc
     1621 gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat
     1681 cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag
     1741 acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc
     1801 cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag
     1861 cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac
     1921 aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg
     1981 gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct
     2041 atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc
     2101 tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta ccgcctttga
     2161 gtgagctgat accgctcgcc gcagccgaac gaccgagcgc agcgagtcag tgagcgagga
     2221 agcggaagag cgcccaatac gcaaaccgcc tctccccgcg cgttggccga ttcattaatg
     2281 cagctggcac gacaggtttc ccgactggaa agcgggcagt gagcgcaacg caattaatac
     2341 gcgtaccgct agccaggaag agtttgtaga aacgcaaaaa ggccatccgt caggatggcc
     2401 ttctgcttag tttgatgcct ggcagtttat ggcgggcgtc ctgcccgcca ccctccgggc
     2461 cgttgcttca caacgttcaa atccgctccc ggcggatttg tcctactcag gagagcgttc
     2521 accgacaaac aacagataaa acgaaaggcc cagtcttccg actgagcctt tcgttttatt
     2581 tgatgcctgg cagttcccta ctctcgcgtt aacgctagca tggatgtttt cccagtcacg
     2641 acgttgtaaa acgacggcca gtcttaagct cgggccccaa ataatgattt tattttgact
     2701 gatagtgacc tgttcgttgc aacaaattga tgagcaatgc ttttttataa tgccaacttt
     2761 gtacaaaaaa gttggcacca tggctgcaaa taagcccaag ggtcagaatt ctttggcttt
     2821 acacaaagtc atcatggtgg gcagtggtgg cgtgggcaag tcagctctga ctctacagtt
     2881 catgtacgat gagtttgtgg aggactatga gcctaccaaa gcagacagct atcggaagaa
     2941 ggtagtgcta gatggggagg aagtccagat cgatatctta gatacagctg ggcaggagga
     3001 ctacgctgca attagagaca actacttccg aagtggggag gggttcctct gtgttttctc
     3061 tattacagaa atggaatcct ttgcagctac agctgacttc agggagcaga ttttaagagt
     3121 aaaagaagat gagaatgttc catttctact ggttggtaac aaatcagatt tagaagataa
     3181 aagacaggtt tctgtagaag aggcaaaaaa cagagctgag cagtggaatg ttaactacgt
     3241 ggaaacatct gctaaaacac gagctaatgt tgacaaggta ttttttgatt taatgagaga
     3301 aattcgagcg agaaagatgg aagacagcaa agaaaagaat ggaaaaaaga agaggaaaag
     3361 tttagccaag agaatcagag aaagatgctg cattttattg ccaactttct t
//
