LOCUS       sequence-162913-        3798 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway Donor vector containing DECR1, used as an over expression 
            control for gene expression analysis. Part of the Target Accelerator
            Plasmid Collection..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3798)
  AUTHORS   Berger AH, Brooks AN, Wu X, Shrestha Y, Chouinard C, Piccioni F, 
            Bagul M, Kamburov A, Imielinski M, Hogstrom L, Zhu C, Yang X, Pantel
            S, Sakai R, Watson J, Kaplan N, Campbell JD, Singh S, Root DE, 
            Narayan R, Natoli T, Lahr DL, Tirosh I, Tamayo P, Getz G, Wong B, 
            Doench J, Subramanian A, Golub TR, Meyerson M, Boehm JS
  TITLE     High-throughput Phenotyping of Lung Cancer Somatic Mutations.
  JOURNAL   Cancer Cell. 2016 Aug 8;30(2):214-28. doi: 
            10.1016/j.ccell.2016.06.022. Epub 2016 Jul 28.
  PUBMED    27478040
REFERENCE   2  (bases 1 to 3798)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.ccell.2016.06.022"; journalName: "Cancer Cell"; date: 
            "2016-08-8- 8"; volume: "30"; issue: "2"; pages: "214-28"
COMMENT     Sequence Label: pDONR223_DECR1_WT_V5
FEATURES             Location/Qualifiers
     source          1..3798
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(108..696)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(188..207)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(789..1580)
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     primer_bind     2011..2027
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     2011..2027
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     2032..2051
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        2032..2050
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    2068..2167
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(3176..3275)
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(3291..3308)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(3291..3307)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3300..3322)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     terminator      3371..3457
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      3549..3576
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     primer_bind     complement(3735..3752)
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 gcggtaatac ggttatccac agaatcaggg gataacgcag gaaagaacat gtgagcaaaa
       61 ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc
      121 cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca
      181 ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg
      241 accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct
      301 catagctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt
      361 gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag
      421 tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa caggattagc
      481 agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa ctacggctac
      541 actagaagaa cagtatttgg tatctgcgct ctgctgaagc cagttacctt cggaaaaaga
      601 gttggtagct cttgatccgg caaacaaacc accgctggta gcggtggttt ttttgtttgc
      661 aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag atcctttgat cttttctacg
      721 gggtctgacg ctcagtggaa cgacgcgtaa ctcacgttaa gggattttgg tcatgggtgg
      781 ctcgagggtt atttgccgac taccttggtg atctcgcctt tcacgtagtg gacaaattct
      841 tccaactgat ctgcgcgcga ggccaagcga tcttcttctt gtccaagata agcctgtcta
      901 gcttcaagta tgacgggctg atactgggcc ggcaggcgct ccattgccca gtcggcagcg
      961 acatccttcg gcgcgatttt gccggttact gcgctgtacc aaatgcggga caacgtaagc
     1021 actacatttc gctcatcgcc agcccagtcg ggcggcgagt tccatagcgt taaggtttca
     1081 tttagcgcct caaatagatc ctgttcagga accggatcaa agagttcctc cgccgctgga
     1141 cctaccaagg caacgctatg ttctcttgct tttgtcagca agatagccag atcaatgtcg
     1201 atcgtggctg gctcgaagat acctgcaaga atgtcattgc gctgccattc tccaaattgc
     1261 agttcgcgct tagctggata acgccacgga atgatgtcgt cgtgcacaac aatggtgact
     1321 tctacagcgc ggagaatctc gctctctcca ggggaagccg aagtttccaa aaggtcgttg
     1381 atcaaagctc gccgcgttgt ttcatcaagc cttacggtca ccgtaaccag caaatcaata
     1441 tcactgtgtg gcttcaggcc gccatccact gcggagccgt acaaatgtac ggccagcaac
     1501 gtcggttcga gatggcgctc gatgacgcca actacctctg atagttgagt cgatacttcg
     1561 gcgatcaccg cttccctcat aatgtttaac tttgttttag ggcgactgcc ctgctgcgta
     1621 acatcgttgc tgctccataa catcaaacat cgacccacgg cgtaacgcgc ttgctgcttg
     1681 gatgcccgag gcatagactg taccccaaaa aaacagtcat aacaagccat gaaaaccgcc
     1741 actgcgccgt taccaccgct gcgttcggtc aaggttctgg accagttgcg tgagcgcata
     1801 cgctacttgc attacagctt acgaaccgaa caggcttatg tccactgggt tcgtgccttc
     1861 atccgtttcc acggtgtgcg tcacccggca accttgggta gcagcgaagt cgaggcattt
     1921 ctgtcctggc tggtctagag gaggcatgat gatatatttt tatcttgtgc aatgtaacat
     1981 cagagatttt gagacacggg ccagagctgc caggaaacag ctatgaccat gtaatacgac
     2041 tcactatagg ggatatcagc tggatggcaa ataatgattt tattttgact gatagtgacc
     2101 tgttcgttgc aacaaattga taagcaatgc tttcttataa tgccaacttt gtacaagaaa
     2161 gttggcaagg aaccttttgt cttcctgatg agttcttcta tggtgtccca ctgctccttg
     2221 gtgacctttc tcaggtcgtt gaattcccct gaaataagta cttcctctcc accgtcaaat
     2281 ttaatgactg ctccattaat ccaagaagca taatcactac aaaggaaagc agcaagattt
     2341 gcgagttctt ctacagtccc caggcgacca cagggaattc tgccaatcat ttctttctca
     2401 aatgttccag ttgggtccag acggctaaag gcacctttgg tttttatagg ccctggttga
     2461 atcacattga atcgcattcc atatttaccc cattcagctg caagagactt gctcatggct
     2521 tccacacctg ctttggcaga agcacttggt actacaaaac ctgaaccagt ctcagcatag
     2581 atagtagtaa tagaaagaaa tgctgctcct ttctgtgctt taattagttg ttttccaatt
     2641 tctagtgtca cgaaggctgt gccatttaga actatgtcag ttatggtttt ccaagcatta
     2701 ggagaaagtc tttcagtagg agaaataaaa ttccctgctg cattgtttat cacaatatta
     2761 ggatgtcctg caactttgat cagttctgac acagtgtttt gaaccatatc aggatccctc
     2821 acatcacact gaattgcatg aaccttattt ccagtttgag aagaaatttg ttctgcggta
     2881 gctttcaaaa catccatctt ccggctggct atcacgcact gagcacctag gctggacaga
     2941 agagttgtca ttcctttacc aaggccagta cctcccccag taatgaatgc cacttttcct
     3001 tgaaaactat taggtggtag catcgctttt tgaagaggtg aaaagaattt agattgcaaa
     3061 gcttcagtgt tttgatataa tatttttgtc ccataactga aaaacctccg aggagcgagg
     3121 ccacagggca gccgggaccc cagagtaaag aaaaccctgg ccggtagctt catggtgcca
     3181 acttttttgt acaaagttgg cattataaaa aagcattgct catcaatttg ttgcaacgaa
     3241 caggtcacta tcagtcaaaa taaaatcatt atttggggcc cgagcttaag actggccgtc
     3301 gttttacaac gtcgtgactg ggaaaacatc catgctagcg ttaacgcgag agtagggaac
     3361 tgccaggcat caaataaaac gaaaggctca gtcggaagac tgggcctttc gttttatctg
     3421 ttgtttgtcg gtgaacgctc tcctgagtag gacaaatccg ccgggagcgg atttgaacgt
     3481 tgtgaagcaa cggcccggag ggtggcgggc aggacgcccg ccataaactg ccaggcatca
     3541 aactaagcag aaggccatcc tgacggatgg cctttttgcg tttctacaaa ctcttcctgg
     3601 ctagcggtac gcgtattaat tgcgttgcgc tcactgcccg ctttccagtc gggaaacctg
     3661 tcgtgccagc tgcattaatg aatcggccaa cgcgcgggga gaggcggttt gcgtattggg
     3721 cgctcttccg cttcctcgct cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg
     3781 gtatcagctc actcaaag
//
