LOCUS       sequence-162920-        4488 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway Donor vector containing CDC45, used as an over expression 
            control for gene expression analysis. Part of the Target Accelerator
            Plasmid Collection..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4488)
  AUTHORS   Berger AH, Brooks AN, Wu X, Shrestha Y, Chouinard C, Piccioni F, 
            Bagul M, Kamburov A, Imielinski M, Hogstrom L, Zhu C, Yang X, Pantel
            S, Sakai R, Watson J, Kaplan N, Campbell JD, Singh S, Root DE, 
            Narayan R, Natoli T, Lahr DL, Tirosh I, Tamayo P, Getz G, Wong B, 
            Doench J, Subramanian A, Golub TR, Meyerson M, Boehm JS
  TITLE     High-throughput Phenotyping of Lung Cancer Somatic Mutations.
  JOURNAL   Cancer Cell. 2016 Aug 8;30(2):214-28. doi: 
            10.1016/j.ccell.2016.06.022. Epub 2016 Jul 28.
  PUBMED    27478040
REFERENCE   2  (bases 1 to 4488)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.ccell.2016.06.022"; journalName: "Cancer Cell"; date: 
            "2016-08-8- 8"; volume: "30"; issue: "2"; pages: "214-28"
COMMENT     Sequence Label: pDONR223_CDC45_WT_V5
FEATURES             Location/Qualifiers
     source          1..4488
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(122..139)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(293..881)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(373..392)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(974..1765)
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     primer_bind     2196..2212
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     2196..2212
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     2217..2236
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        2217..2235
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    2253..2352
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(4051..4150)
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(4166..4183)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(4166..4182)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4175..4197)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     terminator      4246..4332
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      4424..4451
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
ORIGIN
        1 tattaattgc gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg tgccagctgc
       61 attaatgaat cggccaacgc gcggggagag gcggtttgcg tattgggcgc tcttccgctt
      121 cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta tcagctcact
      181 caaaggcggt aatacggtta tccacagaat caggggataa cgcaggaaag aacatgtgag
      241 caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata
      301 ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc
      361 cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg
      421 ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc
      481 tttctcatag ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg
      541 gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc
      601 ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga
      661 ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg
      721 gctacactag aagaacagta tttggtatct gcgctctgct gaagccagtt accttcggaa
      781 aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg
      841 tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt
      901 ctacggggtc tgacgctcag tggaacgacg cgtaactcac gttaagggat tttggtcatg
      961 ggtggctcga gggttatttg ccgactacct tggtgatctc gcctttcacg tagtggacaa
     1021 attcttccaa ctgatctgcg cgcgaggcca agcgatcttc ttcttgtcca agataagcct
     1081 gtctagcttc aagtatgacg ggctgatact gggccggcag gcgctccatt gcccagtcgg
     1141 cagcgacatc cttcggcgcg attttgccgg ttactgcgct gtaccaaatg cgggacaacg
     1201 taagcactac atttcgctca tcgccagccc agtcgggcgg cgagttccat agcgttaagg
     1261 tttcatttag cgcctcaaat agatcctgtt caggaaccgg atcaaagagt tcctccgccg
     1321 ctggacctac caaggcaacg ctatgttctc ttgcttttgt cagcaagata gccagatcaa
     1381 tgtcgatcgt ggctggctcg aagatacctg caagaatgtc attgcgctgc cattctccaa
     1441 attgcagttc gcgcttagct ggataacgcc acggaatgat gtcgtcgtgc acaacaatgg
     1501 tgacttctac agcgcggaga atctcgctct ctccagggga agccgaagtt tccaaaaggt
     1561 cgttgatcaa agctcgccgc gttgtttcat caagccttac ggtcaccgta accagcaaat
     1621 caatatcact gtgtggcttc aggccgccat ccactgcgga gccgtacaaa tgtacggcca
     1681 gcaacgtcgg ttcgagatgg cgctcgatga cgccaactac ctctgatagt tgagtcgata
     1741 cttcggcgat caccgcttcc ctcataatgt ttaactttgt tttagggcga ctgccctgct
     1801 gcgtaacatc gttgctgctc cataacatca aacatcgacc cacggcgtaa cgcgcttgct
     1861 gcttggatgc ccgaggcata gactgtaccc caaaaaaaca gtcataacaa gccatgaaaa
     1921 ccgccactgc gccgttacca ccgctgcgtt cggtcaaggt tctggaccag ttgcgtgagc
     1981 gcatacgcta cttgcattac agcttacgaa ccgaacaggc ttatgtccac tgggttcgtg
     2041 ccttcatccg tttccacggt gtgcgtcacc cggcaacctt gggtagcagc gaagtcgagg
     2101 catttctgtc ctggctggtc tagaggaggc atgatgatat atttttatct tgtgcaatgt
     2161 aacatcagag attttgagac acgggccaga gctgccagga aacagctatg accatgtaat
     2221 acgactcact ataggggata tcagctggat ggcaaataat gattttattt tgactgatag
     2281 tgacctgttc gttgcaacaa attgataagc aatgctttct tataatgcca actttgtaca
     2341 agaaagttgg gtaggacagg agggaaataa gtgcgtccag aaacttgctc cgatcctcag
     2401 ctttcagctc aattactgag aggtcaaaat ggttgtgcag catccgggag ctggtgcttt
     2461 ccgctgcctt ctcaaacgcc ctcccaaaaa agttcttcct gtccgagctg tcggtctctg
     2521 gggggatgcc caccacggtc actgtgccat gctccatgct caggggggca gccatcacca
     2581 ggggcagcag tttgcagcgc cggttctttg tcgaacacac aaaggacttg agcaggtgtt
     2641 tgctgagcag gcttagggat gccggcctag agaacagcat gacatctgga gtgccctcca
     2701 tgagagagca gtacaggaaa ggcccctggg agatgacgag gttggtgcaa aggcagctgg
     2761 caatggtctg ctgggtggct cgcagctgct tcttggcgag ttccaggcca tggtacagct
     2821 tgtccaggtt actcctggag aggctgtcca gagcctggat gaagtgatct gtccctgagc
     2881 catccttctc ggggctctcc atcaaagaca tggtggcaaa gaccacgtcg ctggccagaa
     2941 acttgtgctt gaacccaaaa tgaatgctga aagtctgcac gcgcatgtcc ttcatcccaa
     3001 atttatttgc agactcttca atcatttccc gcaaattctc cttcaaggag atgtccatgg
     3061 cctggaactt ctgcttcacc tgcttcaggg gaagacccat gtctgcaagg aactcctgga
     3121 gccgcttctg tccatgcaca gaccacagct tgaacctggc tgcggtatag ctggtgttgc
     3181 acaggctgtc atggagggac cagtgctggt agagcaccag gcggaggtca tactcaaagg
     3241 agatccgtgt gcagtccacg gagagtgtgt tctcctcatc ctcgttccgg tggttgtggc
     3301 gggaaacgtg gcgctgcagg acaccaacat cagtcacgta tttcatttga gtgatcttgt
     3361 cttgcaccca ctggtctgtt agtccaacga tggcccacca cagcatgtca ttcaggtcct
     3421 tggacagcat ccaagccagc tcaaacatca ccatggctga cgatgtccca tgatattcat
     3481 actgctcgta gtcaaagagg atgtctcttc tccgggcctc ccactctcgc cgctgcctcc
     3541 tccgcatggt ttgctccact atctcctctt ctaaccgtgt gcgcttctca gaaggctctg
     3601 acccatcact gtcatttcct gaatgctctt catcctcctc ttcatccctg aagatgtctt
     3661 cataggcggg aacttcaagg tcatcatctt gtttaatgag taatttgatc tgggtatcgt
     3721 tgtatacatt gacgacattg actggcctat gggtgtcaca cacaaagaat atagtgtctt
     3781 catcaggttg aagaatatcc aataggtcta cattagctcc acagtttatg agaataaaat
     3841 aatgaaactg ttctttatgc tcaagaaatg cagtttcaag ttcttgccac ccagaaactg
     3901 gaaccagcgt atattgcacg tggtcacact ggaacaaggc ctgaaggatc ttgcacgcac
     3961 acagagcatc cacgtccgag gccacgaaga gaaggaccct ctggctctgg accacctcgt
     4021 agaactcttt gcggaaatcg gatacgaaca tgccaacttt tttgtacaaa gttggcatta
     4081 taaaaaagca ttgctcatca atttgttgca acgaacaggt cactatcagt caaaataaaa
     4141 tcattatttg gggcccgagc ttaagactgg ccgtcgtttt acaacgtcgt gactgggaaa
     4201 acatccatgc tagcgttaac gcgagagtag ggaactgcca ggcatcaaat aaaacgaaag
     4261 gctcagtcgg aagactgggc ctttcgtttt atctgttgtt tgtcggtgaa cgctctcctg
     4321 agtaggacaa atccgccggg agcggatttg aacgttgtga agcaacggcc cggagggtgg
     4381 cgggcaggac gcccgccata aactgccagg catcaaacta agcagaaggc catcctgacg
     4441 gatggccttt ttgcgtttct acaaactctt cctggctagc ggtacgcg
//
