LOCUS       sequence-162923-        3867 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway Donor vector containing CCDC90A, used as an over expression 
            control for gene expression analysis. Part of the Target Accelerator
            Plasmid Collection..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3867)
  AUTHORS   Berger AH, Brooks AN, Wu X, Shrestha Y, Chouinard C, Piccioni F, 
            Bagul M, Kamburov A, Imielinski M, Hogstrom L, Zhu C, Yang X, Pantel
            S, Sakai R, Watson J, Kaplan N, Campbell JD, Singh S, Root DE, 
            Narayan R, Natoli T, Lahr DL, Tirosh I, Tamayo P, Getz G, Wong B, 
            Doench J, Subramanian A, Golub TR, Meyerson M, Boehm JS
  TITLE     High-throughput Phenotyping of Lung Cancer Somatic Mutations.
  JOURNAL   Cancer Cell. 2016 Aug 8;30(2):214-28. doi: 
            10.1016/j.ccell.2016.06.022. Epub 2016 Jul 28.
  PUBMED    27478040
REFERENCE   2  (bases 1 to 3867)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.ccell.2016.06.022"; journalName: "Cancer Cell"; date: 
            "2016-08-8- 8"; volume: "30"; issue: "2"; pages: "214-28"
COMMENT     Sequence Label: pDONR223_CCDC90A_WT_V5
FEATURES             Location/Qualifiers
     source          1..3867
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    complement(336..435)
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(451..468)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(451..467)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(460..482)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     terminator      531..617
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      709..736
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     primer_bind     complement(895..912)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1066..1654)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1146..1165)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(1747..2538)
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     primer_bind     2969..2985
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     2969..2985
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     2990..3009
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        2990..3008
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    3026..3125
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
ORIGIN
        1 gcacctccac gcgctgctgc gcccggccgg gggtgcggca tacccgaggc gcgagcgctc
       61 ccagtccccg agctgccggc gcggggctgc ggcggccaag cgcggggagg gcactagcag
      121 gaggaggagc agcggtgagg cacgtgacac gccgccgcgg gccgccgggg cgcgagggcg
      181 cagcgccccc agaccgtcgg agagcgcaga gaggcagcgg cgtgctgagg tttcgctgcc
      241 gcccggtctt ccgctgaggc ccacgggcaa gaagagaagc cgctggcggc cgggcaggcg
      301 ctgggtcctc tggccgccga ccgagccgca gtccatgcca acttttttgt acaaagttgg
      361 cattataaaa aagcattgct catcaatttg ttgcaacgaa caggtcacta tcagtcaaaa
      421 taaaatcatt atttggggcc cgagcttaag actggccgtc gttttacaac gtcgtgactg
      481 ggaaaacatc catgctagcg ttaacgcgag agtagggaac tgccaggcat caaataaaac
      541 gaaaggctca gtcggaagac tgggcctttc gttttatctg ttgtttgtcg gtgaacgctc
      601 tcctgagtag gacaaatccg ccgggagcgg atttgaacgt tgtgaagcaa cggcccggag
      661 ggtggcgggc aggacgcccg ccataaactg ccaggcatca aactaagcag aaggccatcc
      721 tgacggatgg cctttttgcg tttctacaaa ctcttcctgg ctagcggtac gcgtattaat
      781 tgcgttgcgc tcactgcccg ctttccagtc gggaaacctg tcgtgccagc tgcattaatg
      841 aatcggccaa cgcgcgggga gaggcggttt gcgtattggg cgctcttccg cttcctcgct
      901 cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc actcaaaggc
      961 ggtaatacgg ttatccacag aatcagggga taacgcagga aagaacatgt gagcaaaagg
     1021 ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg
     1081 cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg
     1141 actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac
     1201 cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca
     1261 tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt
     1321 gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc
     1381 caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag
     1441 agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac
     1501 tagaagaaca gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt
     1561 tggtagctct tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa
     1621 gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg
     1681 gtctgacgct cagtggaacg acgcgtaact cacgttaagg gattttggtc atgggtggct
     1741 cgagggttat ttgccgacta ccttggtgat ctcgcctttc acgtagtgga caaattcttc
     1801 caactgatct gcgcgcgagg ccaagcgatc ttcttcttgt ccaagataag cctgtctagc
     1861 ttcaagtatg acgggctgat actgggccgg caggcgctcc attgcccagt cggcagcgac
     1921 atccttcggc gcgattttgc cggttactgc gctgtaccaa atgcgggaca acgtaagcac
     1981 tacatttcgc tcatcgccag cccagtcggg cggcgagttc catagcgtta aggtttcatt
     2041 tagcgcctca aatagatcct gttcaggaac cggatcaaag agttcctccg ccgctggacc
     2101 taccaaggca acgctatgtt ctcttgcttt tgtcagcaag atagccagat caatgtcgat
     2161 cgtggctggc tcgaagatac ctgcaagaat gtcattgcgc tgccattctc caaattgcag
     2221 ttcgcgctta gctggataac gccacggaat gatgtcgtcg tgcacaacaa tggtgacttc
     2281 tacagcgcgg agaatctcgc tctctccagg ggaagccgaa gtttccaaaa ggtcgttgat
     2341 caaagctcgc cgcgttgttt catcaagcct tacggtcacc gtaaccagca aatcaatatc
     2401 actgtgtggc ttcaggccgc catccactgc ggagccgtac aaatgtacgg ccagcaacgt
     2461 cggttcgaga tggcgctcga tgacgccaac tacctctgat agttgagtcg atacttcggc
     2521 gatcaccgct tccctcataa tgtttaactt tgttttaggg cgactgccct gctgcgtaac
     2581 atcgttgctg ctccataaca tcaaacatcg acccacggcg taacgcgctt gctgcttgga
     2641 tgcccgaggc atagactgta ccccaaaaaa acagtcataa caagccatga aaaccgccac
     2701 tgcgccgtta ccaccgctgc gttcggtcaa ggttctggac cagttgcgtg agcgcatacg
     2761 ctacttgcat tacagcttac gaaccgaaca ggcttatgtc cactgggttc gtgccttcat
     2821 ccgtttccac ggtgtgcgtc acccggcaac cttgggtagc agcgaagtcg aggcatttct
     2881 gtcctggctg gtctagagga ggcatgatga tatattttta tcttgtgcaa tgtaacatca
     2941 gagattttga gacacgggcc agagctgcca ggaaacagct atgaccatgt aatacgactc
     3001 actatagggg atatcagctg gatggcaaat aatgatttta ttttgactga tagtgacctg
     3061 ttcgttgcaa caaattgata agcaatgctt tcttataatg ccaactttgt acaagaaagt
     3121 tgggtagatc cacaggcgat aaaatcccag agctactgtt aggcacgtaa atatagaccc
     3181 tgctaaatat ttaatattat caagcttgtg tgactcaagc atggttttga ggccagcaac
     3241 ctcagtttcg atcttcctgt ctgtctgggt aagggcccga tcttgctggg catgcaatgc
     3301 cactatttct gttctcaatt ccagcagctt cttttcgttc aatgaataca attcttttac
     3361 tctgctcttt tctaggttga agtctaattt ggtatctgtt cggactttga tcacttcatc
     3421 cattacttgt tgttttaact gatgtagttc gagttttatt ttctcatttt ctgctctgag
     3481 ggctgaaaat tcactcttct ccaaaataat catatccttt ttcacattcg caatctgaga
     3541 cattacttgc tgaaaagtga tttcctgctg catcttggtg accatatctt tgtagacgat
     3601 gtccatgttg gcctccagga tcttgaccaa tgcagacaca atgatttctg cttgttgagt
     3661 agcaaaccca ttgtcttcca gtaagcacac taaggcatga gtgtcgaagt agagtttcct
     3721 gctcccagaa gaggtgaaat ctctcctttt gcgctccagc tgcagggatc cagcagacaa
     3781 gcttagctct ctcctgcagg aaacgagtgc aggcgccggg ccgtggtact ggggaagggc
     3841 gccggcggca gcggcgacgc ccggtga
//
