LOCUS       sequence-162925-        3375 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway Donor vector containing RAB31, used as an over expression 
            control for gene expression analysis. Part of the Target Accelerator
            Plasmid Collection..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3375)
  AUTHORS   Berger AH, Brooks AN, Wu X, Shrestha Y, Chouinard C, Piccioni F, 
            Bagul M, Kamburov A, Imielinski M, Hogstrom L, Zhu C, Yang X, Pantel
            S, Sakai R, Watson J, Kaplan N, Campbell JD, Singh S, Root DE, 
            Narayan R, Natoli T, Lahr DL, Tirosh I, Tamayo P, Getz G, Wong B, 
            Doench J, Subramanian A, Golub TR, Meyerson M, Boehm JS
  TITLE     High-throughput Phenotyping of Lung Cancer Somatic Mutations.
  JOURNAL   Cancer Cell. 2016 Aug 8;30(2):214-28. doi: 
            10.1016/j.ccell.2016.06.022. Epub 2016 Jul 28.
  PUBMED    27478040
REFERENCE   2  (bases 1 to 3375)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.ccell.2016.06.022"; journalName: "Cancer Cell"; date: 
            "2016-08-8- 8"; volume: "30"; issue: "2"; pages: "214-28"
COMMENT     Sequence Label: pDONR223_RAB31_WT_V5
FEATURES             Location/Qualifiers
     source          1..3375
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(50..69)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(51..69)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(74..90)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(74..90)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             521..1312
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      1405..1993
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1894..1913
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     2147..2164
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(2323..2350)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(2442..2528)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     2577..2599
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     2591..2608
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     2592..2608
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    2624..2723
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(join(3309..3375,1..33))
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
ORIGIN
        1 ggtcactatc agtcaaaata aaatcattat ttgccatcca gctgatatcc cctatagtga
       61 gtcgtattac atggtcatag ctgtttcctg gcagctctgg cccgtgtctc aaaatctctg
      121 atgttacatt gcacaagata aaaatatatc atcatgcctc ctctagacca gccaggacag
      181 aaatgcctcg acttcgctgc tacccaaggt tgccgggtga cgcacaccgt ggaaacggat
      241 gaaggcacga acccagtgga cataagcctg ttcggttcgt aagctgtaat gcaagtagcg
      301 tatgcgctca cgcaactggt ccagaacctt gaccgaacgc agcggtggta acggcgcagt
      361 ggcggttttc atggcttgtt atgactgttt ttttggggta cagtctatgc ctcgggcatc
      421 caagcagcaa gcgcgttacg ccgtgggtcg atgtttgatg ttatggagca gcaacgatgt
      481 tacgcagcag ggcagtcgcc ctaaaacaaa gttaaacatt atgagggaag cggtgatcgc
      541 cgaagtatcg actcaactat cagaggtagt tggcgtcatc gagcgccatc tcgaaccgac
      601 gttgctggcc gtacatttgt acggctccgc agtggatggc ggcctgaagc cacacagtga
      661 tattgatttg ctggttacgg tgaccgtaag gcttgatgaa acaacgcggc gagctttgat
      721 caacgacctt ttggaaactt cggcttcccc tggagagagc gagattctcc gcgctgtaga
      781 agtcaccatt gttgtgcacg acgacatcat tccgtggcgt tatccagcta agcgcgaact
      841 gcaatttgga gaatggcagc gcaatgacat tcttgcaggt atcttcgagc cagccacgat
      901 cgacattgat ctggctatct tgctgacaaa agcaagagaa catagcgttg ccttggtagg
      961 tccagcggcg gaggaactct ttgatccggt tcctgaacag gatctatttg aggcgctaaa
     1021 tgaaacctta acgctatgga actcgccgcc cgactgggct ggcgatgagc gaaatgtagt
     1081 gcttacgttg tcccgcattt ggtacagcgc agtaaccggc aaaatcgcgc cgaaggatgt
     1141 cgctgccgac tgggcaatgg agcgcctgcc ggcccagtat cagcccgtca tacttgaagc
     1201 tagacaggct tatcttggac aagaagaaga tcgcttggcc tcgcgcgcag atcagttgga
     1261 agaatttgtc cactacgtga aaggcgagat caccaaggta gtcggcaaat aaccctcgag
     1321 ccacccatga ccaaaatccc ttaacgtgag ttacgcgtcg ttccactgag cgtcagaccc
     1381 cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt
     1441 gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac
     1501 tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg ttcttctagt
     1561 gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct
     1621 gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga
     1681 ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac
     1741 acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg
     1801 agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt
     1861 cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc
     1921 tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg
     1981 gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc
     2041 ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc
     2101 ctttgagtga gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag
     2161 cgaggaagcg gaagagcgcc caatacgcaa accgcctctc cccgcgcgtt ggccgattca
     2221 ttaatgcagc tggcacgaca ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat
     2281 taatacgcgt accgctagcc aggaagagtt tgtagaaacg caaaaaggcc atccgtcagg
     2341 atggccttct gcttagtttg atgcctggca gtttatggcg ggcgtcctgc ccgccaccct
     2401 ccgggccgtt gcttcacaac gttcaaatcc gctcccggcg gatttgtcct actcaggaga
     2461 gcgttcaccg acaaacaaca gataaaacga aaggcccagt cttccgactg agcctttcgt
     2521 tttatttgat gcctggcagt tccctactct cgcgttaacg ctagcatgga tgttttccca
     2581 gtcacgacgt tgtaaaacga cggccagtct taagctcggg ccccaaataa tgattttatt
     2641 ttgactgata gtgacctgtt cgttgcaaca aattgatgag caatgctttt ttataatgcc
     2701 aactttgtac aaaaaagttg gcatgatggc gatacgggag ctcaaagtgt gccttctcgg
     2761 ggacactggg gttgggaaat caagcatcgt gtgtcgattt gtccaggatc actttgacca
     2821 caacatcagc cctactattg gggcatcttt tatgaccaaa actgtgcctt gtggaaatga
     2881 acttcacaag ttcctcatct gggacactgc tggtcaggaa cggtttcatt cattggctcc
     2941 catgtactat cgaggctcag ctgcagctgt tatcgtgtat gatattacca agcaggattc
     3001 attttatacc ttgaagaaat gggtcaagga gctgaaagaa catggtccag aaaacattgt
     3061 aatggccatc gctggaaaca agtgcgacct ctcagatatt agggaggttc ccctgaagga
     3121 tgctaaggaa tacgctgaat ccataggtgc catcgtggtt gagacaagtg caaaaaatgc
     3181 tattaatatc gaagagctct ttcaaggaat cagccgccag atcccaccct tggaccccca
     3241 tgaaaatgga aacaatggaa caatcaaagt tgagaagcca accatgcaag ccagccgccg
     3301 gtgctgttgc ccaactttct tgtacaaagt tggcattata agaaagcatt gcttatcaat
     3361 ttgttgcaac gaaca
//
