LOCUS       sequence-162927-        4902 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway Donor vector containing TRAP1, used as an over expression 
            control for gene expression analysis. Part of the Target Accelerator
            Plasmid Collection..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4902)
  AUTHORS   Berger AH, Brooks AN, Wu X, Shrestha Y, Chouinard C, Piccioni F, 
            Bagul M, Kamburov A, Imielinski M, Hogstrom L, Zhu C, Yang X, Pantel
            S, Sakai R, Watson J, Kaplan N, Campbell JD, Singh S, Root DE, 
            Narayan R, Natoli T, Lahr DL, Tirosh I, Tamayo P, Getz G, Wong B, 
            Doench J, Subramanian A, Golub TR, Meyerson M, Boehm JS
  TITLE     High-throughput Phenotyping of Lung Cancer Somatic Mutations.
  JOURNAL   Cancer Cell. 2016 Aug 8;30(2):214-28. doi: 
            10.1016/j.ccell.2016.06.022. Epub 2016 Jul 28.
  PUBMED    27478040
REFERENCE   2  (bases 1 to 4902)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.ccell.2016.06.022"; journalName: "Cancer Cell"; date: 
            "2016-08-8- 8"; volume: "30"; issue: "2"; pages: "214-28"
COMMENT     Sequence Label: pDONR223_TRAP1_WT_V5
FEATURES             Location/Qualifiers
     source          1..4902
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    complement(198..297)
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(313..330)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(313..329)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(322..344)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     terminator      393..479
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      571..598
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     primer_bind     complement(757..774)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(928..1516)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1008..1027)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(1609..2400)
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     primer_bind     2831..2847
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     2831..2847
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     2852..2871
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        2852..2870
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    2888..2987
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
ORIGIN
        1 gtcctcggcg gtctgcgtgc tgaacagtcg tcctgcctgc aagctccagg ctgggtttcg
       61 cctggggccc aactgggctg tggtcctccg aggacacaga attggttttc ctcccggcac
      121 ggccgccagc gccggcgccc gcagcaaagg ccgcaggcgg cggccccaca gcagcagcgc
      181 ccgcagctcg cgcgccatgc caactttttt gtacaaagtt ggcattataa aaaagcattg
      241 ctcatcaatt tgttgcaacg aacaggtcac tatcagtcaa aataaaatca ttatttgggg
      301 cccgagctta agactggccg tcgttttaca acgtcgtgac tgggaaaaca tccatgctag
      361 cgttaacgcg agagtaggga actgccaggc atcaaataaa acgaaaggct cagtcggaag
      421 actgggcctt tcgttttatc tgttgtttgt cggtgaacgc tctcctgagt aggacaaatc
      481 cgccgggagc ggatttgaac gttgtgaagc aacggcccgg agggtggcgg gcaggacgcc
      541 cgccataaac tgccaggcat caaactaagc agaaggccat cctgacggat ggcctttttg
      601 cgtttctaca aactcttcct ggctagcggt acgcgtatta attgcgttgc gctcactgcc
      661 cgctttccag tcgggaaacc tgtcgtgcca gctgcattaa tgaatcggcc aacgcgcggg
      721 gagaggcggt ttgcgtattg ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc
      781 ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag gcggtaatac ggttatccac
      841 agaatcaggg gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa
      901 ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc cgcccccctg acgagcatca
      961 caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc
     1021 gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata
     1081 cctgtccgcc tttctccctt cgggaagcgt ggcgctttct catagctcac gctgtaggta
     1141 tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca
     1201 gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga
     1261 cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg
     1321 tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagaa cagtatttgg
     1381 tatctgcgct ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg
     1441 caaacaaacc accgctggta gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag
     1501 aaaaaaagga tctcaagaag atcctttgat cttttctacg gggtctgacg ctcagtggaa
     1561 cgacgcgtaa ctcacgttaa gggattttgg tcatgggtgg ctcgagggtt atttgccgac
     1621 taccttggtg atctcgcctt tcacgtagtg gacaaattct tccaactgat ctgcgcgcga
     1681 ggccaagcga tcttcttctt gtccaagata agcctgtcta gcttcaagta tgacgggctg
     1741 atactgggcc ggcaggcgct ccattgccca gtcggcagcg acatccttcg gcgcgatttt
     1801 gccggttact gcgctgtacc aaatgcggga caacgtaagc actacatttc gctcatcgcc
     1861 agcccagtcg ggcggcgagt tccatagcgt taaggtttca tttagcgcct caaatagatc
     1921 ctgttcagga accggatcaa agagttcctc cgccgctgga cctaccaagg caacgctatg
     1981 ttctcttgct tttgtcagca agatagccag atcaatgtcg atcgtggctg gctcgaagat
     2041 acctgcaaga atgtcattgc gctgccattc tccaaattgc agttcgcgct tagctggata
     2101 acgccacgga atgatgtcgt cgtgcacaac aatggtgact tctacagcgc ggagaatctc
     2161 gctctctcca ggggaagccg aagtttccaa aaggtcgttg atcaaagctc gccgcgttgt
     2221 ttcatcaagc cttacggtca ccgtaaccag caaatcaata tcactgtgtg gcttcaggcc
     2281 gccatccact gcggagccgt acaaatgtac ggccagcaac gtcggttcga gatggcgctc
     2341 gatgacgcca actacctctg atagttgagt cgatacttcg gcgatcaccg cttccctcat
     2401 aatgtttaac tttgttttag ggcgactgcc ctgctgcgta acatcgttgc tgctccataa
     2461 catcaaacat cgacccacgg cgtaacgcgc ttgctgcttg gatgcccgag gcatagactg
     2521 taccccaaaa aaacagtcat aacaagccat gaaaaccgcc actgcgccgt taccaccgct
     2581 gcgttcggtc aaggttctgg accagttgcg tgagcgcata cgctacttgc attacagctt
     2641 acgaaccgaa caggcttatg tccactgggt tcgtgccttc atccgtttcc acggtgtgcg
     2701 tcacccggca accttgggta gcagcgaagt cgaggcattt ctgtcctggc tggtctagag
     2761 gaggcatgat gatatatttt tatcttgtgc aatgtaacat cagagatttt gagacacggg
     2821 ccagagctgc caggaaacag ctatgaccat gtaatacgac tcactatagg ggatatcagc
     2881 tggatggcaa ataatgattt tattttgact gatagtgacc tgttcgttgc aacaaattga
     2941 taagcaatgc tttcttataa tgccaacttt gtacaagaaa gttgggcagt gtcgctccag
     3001 ggccttgaca agcagctcat tcaagcggcc caccatggcc ctagggtcgt caacaagtcc
     3061 agcagcaatc atggcgttct cgtatatctg atccaccagc agctgagcca ggccaggctc
     3121 gcttgcgcgc agctgattca gcttcttgat gagcgcgtgc ctggggttga tctccagcgt
     3181 gggctgcagg agctgtgcgc gctcctcctg ggtcttggcc agctgctgca tgcgcaggaa
     3241 gtggcgggca gcccccatct ccagcacggt gaccatggca gggtgggtgt ccagtcggag
     3301 ggtcaccttc acgttggtga cacgcgaccc cagcacattt ctcatccagg ccatgagctc
     3361 ctccgtctcc ttctctgata ggcactcggc ggctggggac ctgtcctcaa acttctcctc
     3421 cttgtagtga tccacgacta tgtccgtctc cacagagatc agcttcttct tgtcaaactc
     3481 acgaaggtgc agcagggtga gctcatcaaa ctgctcaaag cagaagagaa cctctgtgtc
     3541 tttcttcttc atggcctcat agtagggtga gtgctctgcc aggtgacggt tgggggcgca
     3601 caggtagtag atgttgcggg tgccggcccg catgcggctg gcgtattctg agaggctggt
     3661 tagctgcccg gagggcagcg ccgaggactc gtagcgcagc agctttgcta tgtcctcctt
     3721 gacctcctgc tcggtggcgg tcacaatgcc ctcccgcatg aacaggccgt aatcttcaaa
     3781 aaactttgca tacttctcag catctttttt actctggtca atgaagaatt tgatcagcct
     3841 ctgctgtaaa acgtcccgga gtttcctgat gagtgcgctc tcctgcagca gctcccggct
     3901 gaggttcagg ggaatgtcct cactgtccac cacacctcgg atgaagcgca gccacttggg
     3961 caggatgtcc gtggccttgg tctggatgag gactttgcgg ctgtacagtg caacgctgga
     4021 gcccagctcc cggctcacat caaacatgga cggtttcatg tcgggcacgt agaagatgct
     4081 gcggatgttg agcggtgcgt ccgtcttata gtgcagggtg tagcggggct tgtcgtgagc
     4141 ctgcgcgacg tagcggtaga actcctcatg ttgccactca cggacatcct tggggtccat
     4201 catccagatg gcctgcaagg tgttcatccg ccttccattc aagtacaagg ggaagctgac
     4261 gaagttgctg tacttcgtta ccacatctcg cacccgggcc tcgctggaaa actccttgca
     4321 gtcggatttc aggtggatga tgatttttgt cccggttcta actcccgaag cttcggcgat
     4381 ttcaaacact ccagaaccat ctgaaagcca ctggtaaccc aggctccccg gggctgccga
     4441 gcgggaatag acctccactc tgtcagccac catgaaagct gagtagaaac ccactccaaa
     4501 ctggccgatg atcttgctgc tggcctcagc ctggttctgc agagcatcca ggaaggcctt
     4561 tgaccccgat ctggcaatcg tccccaggtt ggacaccagc tcttcctgtg tcatcccgat
     4621 accagtatcc tggatggtga tggtgccttt ctcggcattg gtctgcaagt gaatctccat
     4681 ttctggcagt gcttggccgt cagacaccag tttgtgacgc agtttttcca aggcatcgct
     4741 ggcattggag atcagctccc gtataaacac ctctttttct gagtacaggg accgggcaac
     4801 aatgtccaaa agcttctttg tctcggcctg gaactcatgt ttggaagtgg aaccctgcac
     4861 gctctctgtg ctgctgataa tcgagtgcag gggttcctcc tt
//
