LOCUS       sequence-162928-        1956 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1956)
  AUTHORS   Vale RD, Spudich JA, Griffis ER
  TITLE     Dynamics of myosin, microtubules, and Kinesin-6 at the cortex during
            cytokinesis in Drosophila S2 cells.
  JOURNAL   J Cell Biol. 2009 Sep 7. 186(5):727-38.
  PUBMED    19720876
REFERENCE   2  (bases 1 to 1956)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Cell 
            Biol. 2009 Sep 7. 186(5):727-38."
COMMENT     Sequence Label: pMT-Aurora B-GFP
FEATURES             Location/Qualifiers
     source          1..1956
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    36..60
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(1084..1108)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     regulatory      1116..1125
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(1122..1124,1125..1127,1128..1838)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(1122..1124,1125..1127,1128..1838)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(1167..1188)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(1428..1447)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     1775..1796
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             1877..1918
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             1928..1945
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 ctactagtca gtgtggtgga attctgcaga tatcgacaag tttgtacaaa aaagcaggct
       61 ccgcggccgc ccccttcacc atgacgcttt cccgcgcgaa gcacgccaac cgcaaccacc
      121 tgccgcacct gctggccaag gtgcccgagg agcaccagga gccgatcaag aatatgtgcc
      181 tcaagatgat gtcccacgac gcatacggac agccatacga ttggagtccc cgggactttg
      241 agatgggcgc ccacttggga cgcggcaaat ttggacgtgt ctacttggcg cgggagcgcc
      301 actcgcacta tctggtggcc atgaaggtga tgttcaaaga ggagctgcgc aagggctgcg
      361 tgcagcgtca ggttctacgc gagatcgaga tccagtcgcg actgaagcac ccgcacatcc
      421 tgcgcctgct cacttggttc cacgacgaga gccgcatata tctggccctg gaaattgcgt
      481 ccgagggcga gctgttcaag cacctgcgtg gcgcacccaa tcatcgattc gatgaaccgc
      541 ggtctgcgaa gtacacctac caagtggcga atgccctcaa ctactgtcac ctgaacaacg
      601 tgatccatcg ggatctgaag ccggagaaca ttctgctaac cagcacggac gaccttaagc
      661 tggccgactt tggttggtcg gcgcacacgc cgaacaacaa gcgcaggact ctgtgcggca
      721 ccctggacta tctgccgccg gagatggtgg atgggaactc ctacgacgac tcggtggacc
      781 agtggtgcct gggcattctg tgctatgagt tcgtcgtcgg ctgtccgccc ttcgagtcga
      841 acagcacgga gagcacctac agcaagatcc ggcgcatgga gatctcctac ccatcgcatt
      901 tgtccaaggg ctgcaaggaa ttgattggcg ggctgcttcg caaggaaagc aagggccgca
      961 tcaccttggt ggatgtgatg acccattatt gggtcaaggc gggaatggcc gaaagggagc
     1021 tgcagctgca gaaacgggag cgcggcaagg agaacacggc cagaaataag ggtgggcgcg
     1081 ccgacccagc tttcttgtac aaagtggtga gctccgccac catggtgagc aagggcgagg
     1141 agctgttcac cggggtggtg cccatcctgg tcgagctgga cggcgacgta aacggccaca
     1201 agttcagcgt gtccggcgag ggcgagggcg atgccaccta cggcaagctg accctgaagt
     1261 tcatctgcac caccggcaag ctgcccgtgc cctggcccac cctcgtgacc accctgacct
     1321 acggcgtgca gtgcttcagc cgctaccccg accacatgaa gcagcacgac ttcttcaagt
     1381 ccgccatgcc cgaaggctac gtccaggagc gcaccatctt cttcaaggac gacggcaact
     1441 acaagacccg cgccgaggtg aagttcgagg gcgacaccct ggtgaaccgc atcgagctga
     1501 agggcatcga cttcaaggag gacggcaaca tcctggggca caagctggag tacaactaca
     1561 acagccacaa cgtctatatc atggccgaca agcagaagaa cggcatcaag gtgaacttca
     1621 agatccgcca caacatcgag gacggcagcg tgcagctcgc cgaccactac cagcagaaca
     1681 cccccatcgg cgacggcccc gtgctgctgc ccgacaacca ctacctgagc acccagtccg
     1741 ccctgagcaa agaccccaac gagaagcgcg atcacatggt cctgctggag ttcgtgaccg
     1801 ccgccgggat cactctcggc atggacgagc tgtacaagca ccggtccacg tgacgtaagc
     1861 tagagggccc ttcgaaggta agcctatccc taaccctctc ctcggtctcg attctacgcg
     1921 taccggtcat catcaccatc accattgagt ttaaac
//
