LOCUS       sequence-162929-        4173 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway Donor vector containing RNH1, used as an over expression 
            control for gene expression analysis. Part of the Target Accelerator
            Plasmid Collection..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4173)
  AUTHORS   Berger AH, Brooks AN, Wu X, Shrestha Y, Chouinard C, Piccioni F, 
            Bagul M, Kamburov A, Imielinski M, Hogstrom L, Zhu C, Yang X, Pantel
            S, Sakai R, Watson J, Kaplan N, Campbell JD, Singh S, Root DE, 
            Narayan R, Natoli T, Lahr DL, Tirosh I, Tamayo P, Getz G, Wong B, 
            Doench J, Subramanian A, Golub TR, Meyerson M, Boehm JS
  TITLE     High-throughput Phenotyping of Lung Cancer Somatic Mutations.
  JOURNAL   Cancer Cell. 2016 Aug 8;30(2):214-28. doi: 
            10.1016/j.ccell.2016.06.022. Epub 2016 Jul 28.
  PUBMED    27478040
REFERENCE   2  (bases 1 to 4173)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.ccell.2016.06.022"; journalName: "Cancer Cell"; date: 
            "2016-08-8- 8"; volume: "30"; issue: "2"; pages: "214-28"
COMMENT     Sequence Label: pDONR223_RNH1_WT_V5
FEATURES             Location/Qualifiers
     source          1..4173
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    complement(40..139)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(156..175)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(157..175)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(180..196)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(180..196)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             627..1418
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      1511..2099
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2000..2019
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     2253..2270
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(2429..2456)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(2548..2634)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     2683..2705
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     2697..2714
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     2698..2714
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    2730..2829
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
ORIGIN
        1 ccctggagaa ggacaagcca tccctgaggg tcatctcctg cccaactttc ttgtacaaag
       61 ttggcattat aagaaagcat tgcttatcaa tttgttgcaa cgaacaggtc actatcagtc
      121 aaaataaaat cattatttgc catccagctg atatccccta tagtgagtcg tattacatgg
      181 tcatagctgt ttcctggcag ctctggcccg tgtctcaaaa tctctgatgt tacattgcac
      241 aagataaaaa tatatcatca tgcctcctct agaccagcca ggacagaaat gcctcgactt
      301 cgctgctacc caaggttgcc gggtgacgca caccgtggaa acggatgaag gcacgaaccc
      361 agtggacata agcctgttcg gttcgtaagc tgtaatgcaa gtagcgtatg cgctcacgca
      421 actggtccag aaccttgacc gaacgcagcg gtggtaacgg cgcagtggcg gttttcatgg
      481 cttgttatga ctgttttttt ggggtacagt ctatgcctcg ggcatccaag cagcaagcgc
      541 gttacgccgt gggtcgatgt ttgatgttat ggagcagcaa cgatgttacg cagcagggca
      601 gtcgccctaa aacaaagtta aacattatga gggaagcggt gatcgccgaa gtatcgactc
      661 aactatcaga ggtagttggc gtcatcgagc gccatctcga accgacgttg ctggccgtac
      721 atttgtacgg ctccgcagtg gatggcggcc tgaagccaca cagtgatatt gatttgctgg
      781 ttacggtgac cgtaaggctt gatgaaacaa cgcggcgagc tttgatcaac gaccttttgg
      841 aaacttcggc ttcccctgga gagagcgaga ttctccgcgc tgtagaagtc accattgttg
      901 tgcacgacga catcattccg tggcgttatc cagctaagcg cgaactgcaa tttggagaat
      961 ggcagcgcaa tgacattctt gcaggtatct tcgagccagc cacgatcgac attgatctgg
     1021 ctatcttgct gacaaaagca agagaacata gcgttgcctt ggtaggtcca gcggcggagg
     1081 aactctttga tccggttcct gaacaggatc tatttgaggc gctaaatgaa accttaacgc
     1141 tatggaactc gccgcccgac tgggctggcg atgagcgaaa tgtagtgctt acgttgtccc
     1201 gcatttggta cagcgcagta accggcaaaa tcgcgccgaa ggatgtcgct gccgactggg
     1261 caatggagcg cctgccggcc cagtatcagc ccgtcatact tgaagctaga caggcttatc
     1321 ttggacaaga agaagatcgc ttggcctcgc gcgcagatca gttggaagaa tttgtccact
     1381 acgtgaaagg cgagatcacc aaggtagtcg gcaaataacc ctcgagccac ccatgaccaa
     1441 aatcccttaa cgtgagttac gcgtcgttcc actgagcgtc agaccccgta gaaaagatca
     1501 aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac
     1561 caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg
     1621 taactggctt cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag
     1681 gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac
     1741 cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt
     1801 taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg
     1861 agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc
     1921 ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc
     1981 gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc
     2041 acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa
     2101 acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt
     2161 tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg
     2221 ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag
     2281 agcgcccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc
     2341 acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat acgcgtaccg
     2401 ctagccagga agagtttgta gaaacgcaaa aaggccatcc gtcaggatgg ccttctgctt
     2461 agtttgatgc ctggcagttt atggcgggcg tcctgcccgc caccctccgg gccgttgctt
     2521 cacaacgttc aaatccgctc ccggcggatt tgtcctactc aggagagcgt tcaccgacaa
     2581 acaacagata aaacgaaagg cccagtcttc cgactgagcc tttcgtttta tttgatgcct
     2641 ggcagttccc tactctcgcg ttaacgctag catggatgtt ttcccagtca cgacgttgta
     2701 aaacgacggc cagtcttaag ctcgggcccc aaataatgat tttattttga ctgatagtga
     2761 cctgttcgtt gcaacaaatt gatgagcaat gcttttttat aatgccaact ttgtacaaaa
     2821 aagttggcat gagcctggac atccagagcc tggacatcca gtgtgaggag ctgagcgacg
     2881 ctagatgggc cgagctcctc cctctgctcc agcagtgcca agtggtcagg ctggacgact
     2941 gtggcctcac ggaagcacgg tgcaaggaca tcagctctgc acttcgagtc aaccctgcac
     3001 tggcagagct caacctgcgc agcaacgagc tgggcgatgt cggcgtgcat tgcgtgctcc
     3061 agggcctgca gaccccctcc tgcaagatcc agaagctgag cctccagaac tgctgcctga
     3121 cgggggccgg ctgcggggtc ctgtccagca cactacgcac cctgcccacc ctgcaggagc
     3181 tgcacctcag cgacaacctc ttgggggatg cgggcctgca gctgctctgc gaaggactcc
     3241 tggaccccca gtgccgcctg gaaaagctgc agctggagta ttgcagcctc tcggctgcca
     3301 gctgcgagcc cctggcctcc gtgctcaggg ccaagccgga cttcaaggag ctcacggtta
     3361 gcaacaacga catcaatgag gctggcgttc atgtgctatg ccagggcctg aaggactccc
     3421 cctgccagct ggaggcgctc aagctggaga gctgcggtgt gacatcagac aactgccggg
     3481 acctgtgcgg cattgtggcc tccaaggcct cgctgcggga gctggccctg ggcagcaaca
     3541 agctgggtga tgtgggcatg gcggagctgt gcccagggct gctccacccc agctccaggc
     3601 tcaggaccct gtggatctgg gagtgtggca tcactgccaa gggctgcggg gatctgtgcc
     3661 gtgtcctcag ggccaaggag agcctgaagg agctcagcct ggccggcaac gagctggggg
     3721 atgagggtgc ccgactgttg tgtgagaccc tgctggaacc tggctgccag ctggagtcgt
     3781 tgtgggtgaa gtcctgcagc ttcacagccg cctgctgctc ccacttcagc tcagtgctgg
     3841 cccagaacag gtttctcctg gagctacaga taagcaacaa caggctggag gatgcgggcg
     3901 tgcgggagct gtgccagggc ctgggccagc ctggctctgt gctgcgggtg ctctggttgg
     3961 ccgactgcga tgtgagtgac agcagctgca gcagcctcgc cgcaaccctg ttggccaacc
     4021 acagcctgcg tgagctggac ctcagcaaca actgcctggg ggacgcgggc atcctgcagc
     4081 tggtggagag cgtccggcag ccgggctgcc tcctggagca gctggtcctg tacgacattt
     4141 actggtctga ggagatggag gaccggctgc agg
//
