LOCUS       sequence-162943-         838 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  160bp HDR template for murine CD90.1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 838)
  AUTHORS   Kornete M, Marone R, Jeker LT
  TITLE     Highly Efficient and Versatile Plasmid-Based Gene Editing in Primary
            T Cells.
  JOURNAL   J Immunol. 2018 Feb 14. pii: jimmunol.1701121. doi: 
            10.4049/jimmunol.1701121.
  PUBMED    29445007
REFERENCE   2  (bases 1 to 838)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Immunol. 
            2018 Feb 14. pii: jimmunol.1701121. doi: 10.4049/jimmunol.1701121."
COMMENT     Sequence Label: p213_LTJ_160bpCD90.1Template
FEATURES             Location/Qualifiers
     source          1..838
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(293..310)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(293..309)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(302..324)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(518..537)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     637..659
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(697..715)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 attacgccaa gcttgcatgc aggcctctgc agtcgacggg cccgggatcc gatccaatga
       61 attcccgagc acacgtaccg ctcccgcgtc accctctcca accagcccta tatcaaggtc
      121 cttaccctag ccaacttcac caccaagaat gagggcgact acttttgtga gcttcgagtc
      181 tcgggcgcga atcccatgag ctccaataaa agtatcagtg tgtatagaga tgagactggt
      241 tcccagtcga cattggatct agatgcattc gcgaggtacc gagctcgaat tcactggccg
      301 tcgttttaca acgtcgtgac tgggaaaacc ctggcgttac ccaacttaat cgccttgcag
      361 cacatccccc tttcgccagc tggcgtaata gcgaagaggc ccgcaccgat cgcccttccc
      421 aacagttgcg cagcctgaat ggcgaatggc gcctgatgcg gtattttctc cttacgcatc
      481 tgtgcggtat ttcacaccgc atatggtgca ctctcagtac aatctgctct gatgccgcat
      541 agttaagcca gccccgacac ccgccaacac ccgctgacgc gccctgacgg gcttgtctgc
      601 tcccggcatc cgcttacaga caagctgtga ccgtctccgg gagctgcatg tgtcagaggt
      661 tttcaccgtc atcaccgaaa cgcgcgagac gaaagggcct cgtgatacgc ctatttttat
      721 aggttaatgt catgataata atggtttctt agacgtcagg tggcactttt cggggaaatg
      781 tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcat
//
