LOCUS       sequence-162944-        3981 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway Donor vector containing ACAT2, used as an over expression 
            control for gene expression analysis. Part of the Target Accelerator
            Plasmid Collection..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3981)
  AUTHORS   Berger AH, Brooks AN, Wu X, Shrestha Y, Chouinard C, Piccioni F, 
            Bagul M, Kamburov A, Imielinski M, Hogstrom L, Zhu C, Yang X, Pantel
            S, Sakai R, Watson J, Kaplan N, Campbell JD, Singh S, Root DE, 
            Narayan R, Natoli T, Lahr DL, Tirosh I, Tamayo P, Getz G, Wong B, 
            Doench J, Subramanian A, Golub TR, Meyerson M, Boehm JS
  TITLE     High-throughput Phenotyping of Lung Cancer Somatic Mutations.
  JOURNAL   Cancer Cell. 2016 Aug 8;30(2):214-28. doi: 
            10.1016/j.ccell.2016.06.022. Epub 2016 Jul 28.
  PUBMED    27478040
REFERENCE   2  (bases 1 to 3981)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.ccell.2016.06.022"; journalName: "Cancer Cell"; date: 
            "2016-08-8- 8"; volume: "30"; issue: "2"; pages: "214-28"
COMMENT     Sequence Label: pDONR223_ACAT2_WT_V5
FEATURES             Location/Qualifiers
     source          1..3981
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     802..818
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     802..818
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     823..842
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        823..841
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    complement(2150..2249)
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(2265..2282)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(2265..2281)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2274..2296)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     terminator      2345..2431
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      2523..2550
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     primer_bind     complement(2709..2726)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(2880..3468)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2960..2979)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(3561..3981,1..371))
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
ORIGIN
        1 ggctcgaaga tacctgcaag aatgtcattg cgctgccatt ctccaaattg cagttcgcgc
       61 ttagctggat aacgccacgg aatgatgtcg tcgtgcacaa caatggtgac ttctacagcg
      121 cggagaatct cgctctctcc aggggaagcc gaagtttcca aaaggtcgtt gatcaaagct
      181 cgccgcgttg tttcatcaag ccttacggtc accgtaacca gcaaatcaat atcactgtgt
      241 ggcttcaggc cgccatccac tgcggagccg tacaaatgta cggccagcaa cgtcggttcg
      301 agatggcgct cgatgacgcc aactacctct gatagttgag tcgatacttc ggcgatcacc
      361 gcttccctca taatgtttaa ctttgtttta gggcgactgc cctgctgcgt aacatcgttg
      421 ctgctccata acatcaaaca tcgacccacg gcgtaacgcg cttgctgctt ggatgcccga
      481 ggcatagact gtaccccaaa aaaacagtca taacaagcca tgaaaaccgc cactgcgccg
      541 ttaccaccgc tgcgttcggt caaggttctg gaccagttgc gtgagcgcat acgctacttg
      601 cattacagct tacgaaccga acaggcttat gtccactggg ttcgtgcctt catccgtttc
      661 cacggtgtgc gtcacccggc aaccttgggt agcagcgaag tcgaggcatt tctgtcctgg
      721 ctggtctaga ggaggcatga tgatatattt ttatcttgtg caatgtaaca tcagagattt
      781 tgagacacgg gccagagctg ccaggaaaca gctatgacca tgtaatacga ctcactatag
      841 gggatatcag ctggatggca aataatgatt ttattttgac tgatagtgac ctgttcgttg
      901 caacaaattg ataagcaatg ctttcttata atgccaactt tgtacaagca agttgggcat
      961 tctctctgaa cacacattgc tattcccatc ccacccccaa tgcacagggc tgcaacacca
     1021 cgacttctgc ccattctctc cagtgtgtgt aacagggtca caagaattcg acagccagat
     1081 gctccaagag ggtggcccaa ggctatagcc cctccttcaa tattgacctt ctctgggttt
     1141 aatccaagtt ctttaactat tgcagcagag acagctgcaa aggcttcatt gatttcaaat
     1201 atgtcaacat cttccagtga ccaacctgct tttgtaacag cttgctttat ggctggaatt
     1261 ggtcctattc ccataatgga aggctccaca cccacttggg accaggaaac tatccgtgct
     1321 aaaggtgtaa gtccacgttt atcagcttct gacttcttca taagaacgac agctgcagca
     1381 ccatcattta ttcctgaagc attggctggg gtgactgttc ccgttccatc agtaagaaag
     1441 taaggcttta gcttggacat ggcttctatg ttgctcccat ggcgaggaaa ctcatctgtt
     1501 ttaacttcaa taagacctct tctagttgac accaaaactg gtacaatctc tttgtcaaaa
     1561 tggccagctt tctgtgcatt ctctgtcctg ttctgggaca gaactgcaac cttgtcctga
     1621 tcttctctac tcacttgcca ttttttggct acattttcag ctgtaatacc catatgacag
     1681 ttgtgaaatg catctgtaag accatcacag agtatactgt cagtcagtgg catctcacct
     1741 atctttactc ctgttctcaa gtaagccaag tgaggagcct tgctcatatt ttccatgcct
     1801 cctgcaacca caatgctgga gtctcctatc cctattgact ggactgcaag gcacacagct
     1861 tttaggcctg acccacagat catctggcag ctccatgctg gaacagagta gggaattcct
     1921 gcacccacac tggcttgtct aacaggattc tgcccacagc ctgctgccaa gacatgtcca
     1981 aagatgacct cagacacatc ttccggagcc acagtggccc tcttcaagac ttctttgatg
     2041 acagtggagc ccaggtcctg gacaggaaca gcagctaagg caccattgaa ggaacctatg
     2101 atggtccgcg ccgccgagac gatgaccaca ggatctgagc ctgcattcat gccaactttt
     2161 ttgtacaaag ttggcattat aaaaaagcat tgctcatcaa tttgttgcaa cgaacaggtc
     2221 actatcagtc aaaataaaat cattatttgg ggcccgagct taagactggc cgtcgtttta
     2281 caacgtcgtg actgggaaaa catccatgct agcgttaacg cgagagtagg gaactgccag
     2341 gcatcaaata aaacgaaagg ctcagtcgga agactgggcc tttcgtttta tctgttgttt
     2401 gtcggtgaac gctctcctga gtaggacaaa tccgccggga gcggatttga acgttgtgaa
     2461 gcaacggccc ggagggtggc gggcaggacg cccgccataa actgccaggc atcaaactaa
     2521 gcagaaggcc atcctgacgg atggcctttt tgcgtttcta caaactcttc ctggctagcg
     2581 gtacgcgtat taattgcgtt gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc
     2641 cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat tgggcgctct
     2701 tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca
     2761 gctcactcaa aggcggtaat acggttatcc acagaatcag gggataacgc aggaaagaac
     2821 atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt
     2881 ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg
     2941 cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc
     3001 tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc
     3061 gtggcgcttt ctcatagctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc
     3121 aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac
     3181 tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt
     3241 aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct
     3301 aactacggct acactagaag aacagtattt ggtatctgcg ctctgctgaa gccagttacc
     3361 ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt
     3421 ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg
     3481 atcttttcta cggggtctga cgctcagtgg aacgacgcgt aactcacgtt aagggatttt
     3541 ggtcatgggt ggctcgaggg ttatttgccg actaccttgg tgatctcgcc tttcacgtag
     3601 tggacaaatt cttccaactg atctgcgcgc gaggccaagc gatcttcttc ttgtccaaga
     3661 taagcctgtc tagcttcaag tatgacgggc tgatactggg ccggcaggcg ctccattgcc
     3721 cagtcggcag cgacatcctt cggcgcgatt ttgccggtta ctgcgctgta ccaaatgcgg
     3781 gacaacgtaa gcactacatt tcgctcatcg ccagcccagt cgggcggcga gttccatagc
     3841 gttaaggttt catttagcgc ctcaaataga tcctgttcag gaaccggatc aaagagttcc
     3901 tccgccgctg gacctaccaa ggcaacgcta tgttctcttg cttttgtcag caagatagcc
     3961 agatcaatgt cgatcgtggc t
//
