LOCUS       sequence-162946-        4065 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway Donor vector containing RBM34, used as an over expression 
            control for gene expression analysis. Part of the Target Accelerator
            Plasmid Collection..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4065)
  AUTHORS   Berger AH, Brooks AN, Wu X, Shrestha Y, Chouinard C, Piccioni F, 
            Bagul M, Kamburov A, Imielinski M, Hogstrom L, Zhu C, Yang X, Pantel
            S, Sakai R, Watson J, Kaplan N, Campbell JD, Singh S, Root DE, 
            Narayan R, Natoli T, Lahr DL, Tirosh I, Tamayo P, Getz G, Wong B, 
            Doench J, Subramanian A, Golub TR, Meyerson M, Boehm JS
  TITLE     High-throughput Phenotyping of Lung Cancer Somatic Mutations.
  JOURNAL   Cancer Cell. 2016 Aug 8;30(2):214-28. doi: 
            10.1016/j.ccell.2016.06.022. Epub 2016 Jul 28.
  PUBMED    27478040
REFERENCE   2  (bases 1 to 4065)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.ccell.2016.06.022"; journalName: "Cancer Cell"; date: 
            "2016-08-8- 8"; volume: "30"; issue: "2"; pages: "214-28"
COMMENT     Sequence Label: pDONR223_RBM34_WT_V5
FEATURES             Location/Qualifiers
     source          1..4065
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      50..77
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     primer_bind     complement(236..253)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(407..995)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(487..506)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(1088..1879)
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     primer_bind     2310..2326
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     2310..2326
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     2331..2350
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        2331..2349
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    2367..2466
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(3742..3841)
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(3857..3874)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(3857..3873)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3866..3888)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     terminator      3937..4023
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
ORIGIN
        1 gggtggcggg caggacgccc gccataaact gccaggcatc aaactaagca gaaggccatc
       61 ctgacggatg gcctttttgc gtttctacaa actcttcctg gctagcggta cgcgtattaa
      121 ttgcgttgcg ctcactgccc gctttccagt cgggaaacct gtcgtgccag ctgcattaat
      181 gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc
      241 tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg
      301 cggtaatacg gttatccaca gaatcagggg ataacgcagg aaagaacatg tgagcaaaag
      361 gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc
      421 gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag
      481 gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga
      541 ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc
      601 atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg
      661 tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt
      721 ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca
      781 gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca
      841 ctagaagaac agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag
      901 ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca
      961 agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg
     1021 ggtctgacgc tcagtggaac gacgcgtaac tcacgttaag ggattttggt catgggtggc
     1081 tcgagggtta tttgccgact accttggtga tctcgccttt cacgtagtgg acaaattctt
     1141 ccaactgatc tgcgcgcgag gccaagcgat cttcttcttg tccaagataa gcctgtctag
     1201 cttcaagtat gacgggctga tactgggccg gcaggcgctc cattgcccag tcggcagcga
     1261 catccttcgg cgcgattttg ccggttactg cgctgtacca aatgcgggac aacgtaagca
     1321 ctacatttcg ctcatcgcca gcccagtcgg gcggcgagtt ccatagcgtt aaggtttcat
     1381 ttagcgcctc aaatagatcc tgttcaggaa ccggatcaaa gagttcctcc gccgctggac
     1441 ctaccaaggc aacgctatgt tctcttgctt ttgtcagcaa gatagccaga tcaatgtcga
     1501 tcgtggctgg ctcgaagata cctgcaagaa tgtcattgcg ctgccattct ccaaattgca
     1561 gttcgcgctt agctggataa cgccacggaa tgatgtcgtc gtgcacaaca atggtgactt
     1621 ctacagcgcg gagaatctcg ctctctccag gggaagccga agtttccaaa aggtcgttga
     1681 tcaaagctcg ccgcgttgtt tcatcaagcc ttacggtcac cgtaaccagc aaatcaatat
     1741 cactgtgtgg cttcaggccg ccatccactg cggagccgta caaatgtacg gccagcaacg
     1801 tcggttcgag atggcgctcg atgacgccaa ctacctctga tagttgagtc gatacttcgg
     1861 cgatcaccgc ttccctcata atgtttaact ttgttttagg gcgactgccc tgctgcgtaa
     1921 catcgttgct gctccataac atcaaacatc gacccacggc gtaacgcgct tgctgcttgg
     1981 atgcccgagg catagactgt accccaaaaa aacagtcata acaagccatg aaaaccgcca
     2041 ctgcgccgtt accaccgctg cgttcggtca aggttctgga ccagttgcgt gagcgcatac
     2101 gctacttgca ttacagctta cgaaccgaac aggcttatgt ccactgggtt cgtgccttca
     2161 tccgtttcca cggtgtgcgt cacccggcaa ccttgggtag cagcgaagtc gaggcatttc
     2221 tgtcctggct ggtctagagg aggcatgatg atatattttt atcttgtgca atgtaacatc
     2281 agagattttg agacacgggc cagagctgcc aggaaacagc tatgaccatg taatacgact
     2341 cactataggg gatatcagct ggatggcaaa taatgatttt attttgactg atagtgacct
     2401 gttcgttgca acaaattgat aagcaatgct ttcttataat gccaactttg tacaagaaag
     2461 ttgggtattt ctgttttctc tgtttcttag ggcgtccact tttcttctgt cctttcttct
     2521 tcgttttaag gagaacagct ttttctccaa taaataagct tttaggatgt ccttctgcag
     2581 ttttggaagt aaaattaagt ccctgcttag gtttactgac attcttcaat cgtggatttg
     2641 aattttgttg tttaaatttt tctttattaa cagaacgcat gactctgagt tttctcccca
     2701 tgagttcaga attatttaat ttcagagcaa gatgaacaga atctgtattc tcaaagagca
     2761 catagccaaa ccctttgccg atgcctgtca ttttgtctct cacaatcctc acggccatga
     2821 tacttccaca gtccagaaag tgcttctcaa tggcagattc ttcaacttta taagggagat
     2881 tccccacaaa aaccgatctc ttgtctctag atgaggtctc agatgcgaga tcaactctaa
     2941 tacgaaatcc atctgcaatc tgggccccat ttcttttcaa tgcttgcgtg gcagcactct
     3001 cctccttaaa cacaacatag gcattaatat ttttctgatc aggatgaatt ttacgtttta
     3061 ttgctgccaa ctttttggat agcgttccct ctgctggaat cagagaacga aatcgtacag
     3121 attctatttg tccatactct ttaaaaaacg acttcagctt cttcttatta catgtaacag
     3181 gcaaattccc aacaaacaca gttctctcat tctttaatct ctcttcttct tggttgattt
     3241 gaattttctt tctttgactg acaactgtgt cttctgtgtc atcaagtatt tttctatctg
     3301 ctactttaac accaggttga gaatttttcc ttttctgccc ttgtttctgg tgaatttctt
     3361 cttctaaatc agcactcgct agagcgcttt ccctgtctgc caactttttt tctgcgttag
     3421 tgtgtttctt cttcgctttc acttttttgg caggttcttg cgaaagtggt ctttcaatct
     3481 gggatgtact ttcttcctcc tcattccgtt tcgttttttt gatggtttgt ttaggcacag
     3541 gcacgtacac gggttgaatc tggggctcca gagaactgaa gagggacgcc agccgaccgg
     3601 tgccacctct ggaatggtgt tcgccgcgaa ataagctact ggcgacctgt ccaagcctgt
     3661 agtcttccgg cggactcccg cgaacgccgt cgtcaggatt ctctccctcc tggacacttc
     3721 tctttctctt ccgtttgctc atgccaactt ttttgtacaa agttggcatt ataaaaaagc
     3781 attgctcatc aatttgttgc aacgaacagg tcactatcag tcaaaataaa atcattattt
     3841 ggggcccgag cttaagactg gccgtcgttt tacaacgtcg tgactgggaa aacatccatg
     3901 ctagcgttaa cgcgagagta gggaactgcc aggcatcaaa taaaacgaaa ggctcagtcg
     3961 gaagactggg cctttcgttt tatctgttgt ttgtcggtga acgctctcct gagtaggaca
     4021 aatccgccgg gagcggattt gaacgttgtg aagcaacggc ccgga
//
