LOCUS       sequence-162948-         962 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  This plasmid can be used as a donor for subcloning into Gateway 
            destination vectors..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 962)
  AUTHORS   Chymkowitch P, Nguea AP, Aanes H, Koehler CJ, Thiede B, Lorenz S, 
            Meza-Zepeda LA, Klungland A, Enserink JM
  TITLE     Sumoylation of Rap1 mediates the recruitment of TFIID to promote 
            transcription of ribosomal protein genes.
  JOURNAL   Genome Res. 2015 Jun;25(6):897-906. doi: 10.1101/gr.185793.114. Epub
            2015 Mar 23.
  PUBMED    25800674
REFERENCE   2  (bases 1 to 962)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1101/gr.185793.114"; journalName: "Genome Res"; date: "2015-06";
            volume: "25"; issue: "6"; pages: "897-906"
COMMENT     Sequence Label: pDONR221-rap1-K651R
FEATURES             Location/Qualifiers
     source          1..962
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    65..89
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 tttattttga ctgatagtga cctgttcgtt gcaacaaatt gatgagcaat gcttttttat
       61 aatgccaact ttgtacaaga aagctggcta caaaatgtct agtccagatg attttgaaac
      121 tgcaccagca gaatatgttg atgcattgga tccgagtatg gtcgttgttg acagtggttc
      181 tgctgcagtc acagcgccat cggattcagc cgctgaggta aaagctaatc aaaacgaaga
      241 aaacactggt gctactgctg ctgaaactag tgagaaagtg gatcagacgg aggtcgaaaa
      301 gaaagatgat gatgatacca ctgaagtcgg tgtaacaact accacgcctt ccattgctga
      361 tactgctgct accgctaaca ttgcttcgac atcaggtgca tctgttactg aacctactac
      421 tgatgacacc gctgctgatg agaaaaagga acaagtaagt ggtcctcctc tgtcaaatat
      481 gaaattctat cttaatcgcg acgcggatgc gcatgactct ttaaatgata ttgatcaatt
      541 agcgcgtctg attagagcaa acggtggtga agtgctagac tccaagccaa gagaatcaaa
      601 agagaatgtt ttcatcgtgt caccctataa tcataccaat ttaccaacgg taactccgac
      661 ttatattaag gcatgttgtc aaagcaactc gctactgaac atggaaaatt atttagtacc
      721 atacgataac tttagagaag ttgtcgatag taggttgcag gaggaatcgc attctaatgg
      781 tgtagataat agcaatagca atagcgataa caaggattct atcaggccca aaacagaaat
      841 aatctctacg aatactaatg gcgctacgga agactcaacc agcgaaaaag ttatggtaga
      901 cgctgaacaa caagctagat tacaagagca agctcagctc cttcgtcaac atgtaagcag
      961 ca
//
