LOCUS       sequence-162949-         972 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  This plasmid can be used as a donor for subcloning into Gateway 
            destination vectors..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 972)
  AUTHORS   Chymkowitch P, Nguea AP, Aanes H, Koehler CJ, Thiede B, Lorenz S, 
            Meza-Zepeda LA, Klungland A, Enserink JM
  TITLE     Sumoylation of Rap1 mediates the recruitment of TFIID to promote 
            transcription of ribosomal protein genes.
  JOURNAL   Genome Res. 2015 Jun;25(6):897-906. doi: 10.1101/gr.185793.114. Epub
            2015 Mar 23.
  PUBMED    25800674
REFERENCE   2  (bases 1 to 972)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1101/gr.185793.114"; journalName: "Genome Res"; date: "2015-06";
            volume: "25"; issue: "6"; pages: "897-906"
COMMENT     Sequence Label: pDONR221-rap1-K651R
FEATURES             Location/Qualifiers
     source          1..972
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    12..111
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
ORIGIN
        1 cagctggatg gcaaataatg attttatttt gactgatagt gacctgttcg ttgcaacaaa
       61 ttgataagca atgctttctt ataatgccaa ctttgtacaa gaaagctggg ttcataacag
      121 gtccttctca aaaaatcttt tcctcatttc cattctacca gttccatgct ttttaaccag
      181 tttccttatt tgttcttgat cattgctttt tagcgattcg tcgtcatcat gtgtccaaat
      241 accaggaacg ttgggaggag gattaacatt gtccttaaac atgttcaaga aatagcgagg
      301 aaagaccatc agatccccgg ataaacacgt caatatgctc gtactgaaat ttttacgtat
      361 accagtttca tcgcaaagat cctgtaccag tttttcagcc tgtgatggct cataatctcc
      421 tgaaataact tcatgaagtt tatccataaa ttgtctcgta ctgccaaaat gaaagaacaa
      481 tggcaggaaa agatctgttg ttgcaatttt tggtggaaag cttatggtat caggatcaat
      541 attatcatat atatcttcat tcgaaaaatc gcttcttata gcttccgcaa tctcgtgtgg
      601 atactcaaag ggcaaactat ttgaaatatt ggatagatca tttcttaaac tggaaatgaa
      661 attcatggta tcttcgtcca gtaattcgtt ttctggtata gcgtaagagt tggaagcagc
      721 ggcagcagca gcggcagcag cattagcgga cgctgcattg gcagtaggct gaacatttct
      781 ttgagaacta taatttcttg ccctcttggc ggcagagtta taattgccag gagtgggaac
      841 gccaggcctc ttgggtctat tggtgaggtt cttcatcggt tccggctctc tattctgagc
      901 tttttcagct tcataataac taatataatc atcaatgccg taagcgagaa gaaacttcct
      961 aaacctatct ct
//
