LOCUS       sequence-162951-         997 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  This plasmid can be used as a donor for subcloning into Gateway 
            destination vectors..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 997)
  AUTHORS   Chymkowitch P, Nguea AP, Aanes H, Koehler CJ, Thiede B, Lorenz S, 
            Meza-Zepeda LA, Klungland A, Enserink JM
  TITLE     Sumoylation of Rap1 mediates the recruitment of TFIID to promote 
            transcription of ribosomal protein genes.
  JOURNAL   Genome Res. 2015 Jun;25(6):897-906. doi: 10.1101/gr.185793.114. Epub
            2015 Mar 23.
  PUBMED    25800674
REFERENCE   2  (bases 1 to 997)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1101/gr.185793.114"; journalName: "Genome Res"; date: "2015-06";
            volume: "25"; issue: "6"; pages: "897-906"
COMMENT     Sequence Label: pDONR221-RAP1
FEATURES             Location/Qualifiers
     source          1..997
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    12..111
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
ORIGIN
        1 cagctggatg gcaaataatg attttatttt gactgatagt gacctgttcg ttgcaacaaa
       61 ttgataagca atgctttctt ataatgccaa ctttgtacaa gaaagctggg tttaacaggt
      121 ccttctcaaa aaatcttttc ctcatttcca ttctaccagt tccatgcttt ttaaccagtt
      181 tccttatttg ttcttgatca ttgcttttta gcgattcgtc gtcatcatgt gtccaaatac
      241 caggaacgtt gggaggagga ttaacattgt ccttaaacat gttcaagaaa tagcgaggaa
      301 agaccatcag atccccggat aaacacgtca atatgctcgt actgaaattt ttacgtatac
      361 cagtttcatc gcaaagatcc tgtaccagtt tttcagcctg tgatggctca taatctcctg
      421 aaataacttc atgaagttta tccataaatt gtctcgtact gccaaaatga aagaacaatg
      481 gcaggaaaag atctgttgtt gcaatttttg gtggaaagct tatggtatca ggatcaatat
      541 tatcatatat atcttcattc gaaaaatcgc ttcttatagc ttccgcaatc tcgtgtggat
      601 actcaaaggg caaactattt gaaatattgg atagatcatt ctttaaactg gaaatgaaat
      661 tcatggtatc ttcgtccagt aattcgtttt ctggtatagc gtaagagttg gaagcagcgg
      721 cagcagcagc ggcagcagca ttagcggacg ctgcattggc agtaggctga acatttcttt
      781 gagaactata atttcttgcc ctcttggcgg cagagttata attgccagga gtgggaacgc
      841 caggcctctt gggtctattg gtgaggttct tcatcggttc cggctctcta ttctgagctt
      901 tttcagcttc ataataacta atataatcat caatgccgta agcgagaaga aacttcctaa
      961 acctatctct ccaagcattc tcagtatgtg ctgcatg
//
