LOCUS       sequence-162952-        5277 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  This plasmid can be used as a donor for subcloning into Gateway 
            destination vectors..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5277)
  AUTHORS   Chymkowitch P, Nguea AP, Aanes H, Koehler CJ, Thiede B, Lorenz S, 
            Meza-Zepeda LA, Klungland A, Enserink JM
  TITLE     Sumoylation of Rap1 mediates the recruitment of TFIID to promote 
            transcription of ribosomal protein genes.
  JOURNAL   Genome Res. 2015 Jun;25(6):897-906. doi: 10.1101/gr.185793.114. Epub
            2015 Mar 23.
  PUBMED    25800674
REFERENCE   2  (bases 1 to 5277)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1101/gr.185793.114"; journalName: "Genome Res"; date: "2015-06";
            volume: "25"; issue: "6"; pages: "897-906"
COMMENT     Sequence Label: pDONR221-RAP1
FEATURES             Location/Qualifiers
     source          1..5277
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     43..65
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     57..74
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     58..74
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    90..189
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(2677..2776)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(2793..2812)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(2794..2812)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2817..2833)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2817..2833)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             3264..4055
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      4148..4736
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4637..4656
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     4890..4907
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(5066..5093)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(5185..5271)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
ORIGIN
        1 ggcagttccc tactctcgcg ttaacgctag catggatgtt ttcccagtca cgacgttgta
       61 aaacgacggc cagtcttaag ctcgggcccc aaataatgat tttattttga ctgatagtga
      121 cctgttcgtt gcaacaaatt gatgagcaat gcttttttat aatgccaact ttgtacaaaa
      181 aagcaggcta caaaatgtct agtccagatg attttgaaac tgcaccagca gaatatgttg
      241 atgcattgga tccgagtatg gtcgttgttg acagtggttc tgctgcagtc acagcgccat
      301 cggattcagc cgctgaggta aaagctaatc aaaacgaaga aaacactggt gctactgctg
      361 ctgaaactag tgagaaagtg gatcagacgg aggtcgaaaa gaaagatgat gatgatacca
      421 ctgaagtcgg tgtaacaact accacgcctt ccattgctga tactgctgct accgctaaca
      481 ttgcttcgac atcaggtgca tctgttactg aacctactac tgatgacacc gctgctgatg
      541 agaaaaagga acaagtaagt ggtcctcctc tgtcaaatat gaaattctat cttaatcgcg
      601 acgcggatgc gcatgactct ttaaatgata ttgatcaatt agcgcgtctg attagagcaa
      661 acggtggtga agtgctagac tccaagccaa gagaatcaaa agagaatgtt ttcatcgtgt
      721 caccctataa tcataccaat ttaccaacgg taactccgac ttatattaag gcatgttgtc
      781 aaagcaactc gctactgaac atggaaaatt atttagtacc atacgataac tttagagaag
      841 ttgtcgatag taggttgcag gaggaatcgc attctaatgg tgtagataat agcaatagca
      901 atagcgataa caaggattct atcaggccca aaacagaaat aatctctacg aatactaatg
      961 gcgctacgga agactcaacc agcgaaaaag ttatggtaga cgctgaacaa caagctagat
     1021 tacaagagca agctcagctc cttcgtcaac atgtaagcag caccgcatca atcacaagcg
     1081 gagggcacaa tgacttggtc cagatcgaac aacctcaaaa agatacctcc aataataata
     1141 atagcaacgt caacgacgaa gacaatgatc ttttgacaca ggacaacaac cctcaaacag
     1201 cagatgaggg gaatgcatct tttcaagcac aaaggtccat gatttcgaga ggcgctttgc
     1261 cctcccacaa taaagcttct tttacagatg aggaagatga gtttattttg gatgttgtga
     1321 gaaaaaatcc aaccaggcgt acaacacata ctctttacga tgaaatatcc cattatgtgc
     1381 ctaaccacac gggtaattct attaggcacc gatttagagt ctatctttcc aaaagactag
     1441 agtacgttta tgaggttgac aagtttggta aattggttag agacgacgat ggaaatctga
     1501 taaagactaa agttttgcca ccatcaatta aaaggaaatt ttcagcagat gaagattaca
     1561 ctttggcaat cgcggtcaag aagcagtttt atcgtgattt gtttcaaatc gaccctgata
     1621 ctggaagatc acttatcaca gatgaggata cacccactgc tatagcgagg aggaatatga
     1681 caatggatcc aaatcatgtg ccagggagtg aacccaactt tgcagcttac agaacccaaa
     1741 gccgtagggg accgattgca cgagaatttt tcaagcattt tgccgaagag catgcagcac
     1801 atactgagaa tgcttggaga gataggttta ggaagtttct tctcgcttac ggcattgatg
     1861 attatattag ttattatgaa gctgaaaaag ctcagaatag agagccggaa ccgatgaaga
     1921 acctcaccaa tagacccaag aggcctggcg ttcccactcc tggcaattat aactctgccg
     1981 ccaagagggc aagaaattat agttctcaaa gaaatgttca gcctactgcc aatgcagcgt
     2041 ccgctaatgc tgctgccgct gctgctgccg ctgcttccaa ctcttacgct ataccagaaa
     2101 acgaattact ggacgaagat accatgaatt tcatttccag tttaaagaat gatctatcca
     2161 atatttcaaa tagtttgccc tttgagtatc cacacgagat tgcggaagct ataagaagcg
     2221 atttttcgaa tgaagatata tatgataata ttgatcctga taccataagc tttccaccaa
     2281 aaattgcaac aacagatctt ttcctgccat tgttctttca ttttggcagt acgagacaat
     2341 ttatggataa acttcatgaa gttatttcag gagattatga gccatcacag gctgaaaaac
     2401 tggtacagga tctttgcgat gaaactggta tacgtaaaaa tttcagtacg agcatattga
     2461 cgtgtttatc cggggatctg atggtctttc ctcgctattt cttgaacatg tttaaggaca
     2521 atgttaatcc tcctcccaac gttcctggta tttggacaca tgatgacgac gaatcgctaa
     2581 aaagcaatga tcaagaacaa ataaggaaac tggttaaaaa gcatggaact ggtagaatgg
     2641 aaatgaggaa aagatttttt gagaaggacc tgttaaaccc agctttcttg tacaaagttg
     2701 gcattataag aaagcattgc ttatcaattt gttgcaacga acaggtcact atcagtcaaa
     2761 ataaaatcat tatttgccat ccagctgata tcccctatag tgagtcgtat tacatggtca
     2821 tagctgtttc ctggcagctc tggcccgtgt ctcaaaatct ctgatgttac attgcacaag
     2881 ataaaaatat atcatcatgc ctcctctaga ccagccagga cagaaatgcc tcgacttcgc
     2941 tgctgcccaa ggttgccggg tgacgcacac cgtggaaacg gatgaaggca cgaacccagt
     3001 ggacataagc ctgttcggtt cgtaagctgt aatgcaagta gcgtatgcgc tcacgcaact
     3061 ggtccagaac cttgaccgaa cgcagcggtg gtaacggcgc agtggcggtt ttcatggctt
     3121 gttatgactg tttttttggg gtacagtcta tgcctcgggc atccaagcag caagcgcgtt
     3181 acgccgtggg tcgatgtttg atgttatgga gcagcaacga tgttacgcag cagggcagtc
     3241 gccctaaaac aaagttaaac atcatgaggg aagcggtgat cgccgaagta tcgactcaac
     3301 tatcagaggt agttggcgtc atcgagcgcc atctcgaacc gacgttgctg gccgtacatt
     3361 tgtacggctc cgcagtggat ggcggcctga agccacacag tgatattgat ttgctggtta
     3421 cggtgaccgt aaggcttgat gaaacaacgc ggcgagcttt gatcaacgac cttttggaaa
     3481 cttcggcttc ccctggagag agcgagattc tccgcgctgt agaagtcacc attgttgtgc
     3541 acgacgacat cattccgtgg cgttatccag ctaagcgcga actgcaattt ggagaatggc
     3601 agcgcaatga cattcttgca ggtatcttcg agccagccac gatcgacatt gatctggcta
     3661 tcttgctgac aaaagcaaga gaacatagcg ttgccttggt aggtccagcg gcggaggaac
     3721 tctttgatcc ggttcctgaa caggatctat ttgaggcgct aaatgaaacc ttaacgctat
     3781 ggaactcgcc gcccgactgg gctggcgatg agcgaaatgt agtgcttacg ttgtcccgca
     3841 tttggtacag cgcagtaacc ggcaaaatcg cgccgaagga tgtcgctgcc gactgggcaa
     3901 tggagcgcct gccggcccag tatcagcccg tcatacttga agctagacag gcttatcttg
     3961 gacaagaaga agatcgcttg gcctcgcgcg cagatcagtt ggaagaattt gtccactacg
     4021 tgaaaggcga gatcaccaag gtagtcggca aataaccctc gagccaccca tgaccaaaat
     4081 cccttaacgt gagttacgcg tcgttccact gagcgtcaga ccccgtagaa aagatcaaag
     4141 gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac
     4201 cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa
     4261 ctggcttcag cagagcgcag ataccaaata ctgtccttct agtgtagccg tagttaggcc
     4321 accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag
     4381 tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac
     4441 cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc
     4501 gaacgaccta caccgaactg agatacctac agcgtgagca ttgagaaagc gccacgcttc
     4561 ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca
     4621 cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc
     4681 tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg
     4741 ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct
     4801 ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata
     4861 ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaagagc
     4921 gcccaatacg caaaccgcct ctccccgcgc gttggccgat tcattaatgc agctggcacg
     4981 acaggtttcc cgactggaaa gcgggcagtg agcgcaacgc aattaatacg cgtaccgcta
     5041 gccaggaaga gtttgtagaa acgcaaaaag gccatccgtc aggatggcct tctgcttagt
     5101 ttgatgcctg gcagtttatg gcgggcgtcc tgcccgccac cctccgggcc gttgcttcac
     5161 aacgttcaaa tccgctcccg gcggatttgt cctactcagg agagcgttca ccgacaaaca
     5221 acagataaaa cgaaaggccc agtcttccga ctgagccttt cgttttattt gatgcct
//
