LOCUS       sequence-162961-         843 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Inducible expression of redox sensitive GFP in bacteria.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 843)
  AUTHORS   Cannon MB, Remington SJ
  TITLE     Re-engineering redox-sensitive green fluorescent protein for 
            improved response rate.
  JOURNAL   Protein Sci. 2006 Jan;15(1):45-57. Epub 2005 Dec 1.
  PUBMED    16322566
REFERENCE   2  (bases 1 to 843)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Protein 
            Sci."; date: "2006-01"; volume: "15(1)"; pages: "45-57. Epub 2005 
            Dec 1"
COMMENT     Sequence Label: pRSETB-roGFP1-R7
FEATURES             Location/Qualifiers
     source          1..843
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(71..784)
                     /codon_start=1
                     /product="S65T variant of Aequorea victoria green 
                     fluorescent protein (Heim et al., 1995)"
                     /label=GFP (S65T)
                     /note="excitable with blue light"
                     /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FISTTGKLPVPWPTLVTTFSYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNCHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLKTCSALSKDPNEKRDHMVLLE
                     RVTAAGITHGMDELYK"
     primer_bind     726..754
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
     CDS             complement(join(803..817,818..826))
                     /codon_start=1
                     /product="Xpress(TM) epitope tag, including an enterokinase
                     recognition and cleavage site"
                     /label=Xpress(TM) tag
                     /translation="DLYDDDDK"
ORIGIN
        1 ttgttagcag ccggatcaag cttcgaattc catggtacca gctgcagatc tcgagctcgg
       61 atccttatta tttgtatagt tcatccatgc catgtgtaat cccagcagct gttacgcgct
      121 caagaaggac catgtggtct ctcttttcgt tgggatcttt cgaaagggca gagcatgtct
      181 tcaggtaatg gttgtctggt aaaaggacag ggccatcgcc aattggagta ttttgttgat
      241 aatggtctgc tagttgaacg cttccatctt caatgttgtg tctaattttg aagttaactt
      301 tgattccatt cttttgtttg tctgccatga tgtatacatt gtggcagtta tagttgtatt
      361 ccaatttgtg tccaagaatg tttccatctt ctttaaaatc aatacctttt aactcgattc
      421 tattaacaag ggtatcacct tcaaacttga cttcagcacg tgtcttgtag ttcccgtcat
      481 ctttgaaaaa tatagttctt tcctgtacat aaccttcggg catggcactc ttgaaaaagt
      541 catgccgttt catatgatct gggtatcttg aaaagcattg aacaccataa ctgaaagtag
      601 tgacaagtgt tggccatgga acaggtagtt ttccagtagt ggaaataaat ttaagggtaa
      661 gttttccgta tgttgcatca ccttcaccct ctccactgac agaaaatttg tgcccattaa
      721 catcaccatc taattcaaca agaattggga caactccagt gaaaagttct tctcctttac
      781 tcatgaattc agcgggggga tccttatcgt catcgtcgta cagatcccga cccatttgct
      841 gtc
//
