LOCUS       sequence-162973-         986 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses nadV from Haemophilus ducreyi in Escherichia coli.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 986)
  AUTHORS   Marinescu GC, Popescu RG, Dinischiotu A
  TITLE     Size Exclusion Chromatography Method for Purification of 
            Nicotinamide Mononucleotide (NMN) from Bacterial Cells.
  JOURNAL   Sci Rep. 2018 Mar 13;8(1):4433. doi: 10.1038/s41598-018-22806-8.
  PUBMED    29535407
REFERENCE   2  (bases 1 to 986)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Rep."; 
            date: "2018-03-13"; volume: "8(1):4433. doi"; pages: " 
            10.1038/s41598-018-22806-8"
COMMENT     Sequence Label: pET28a-hdNadV
FEATURES             Location/Qualifiers
     source          1..986
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     65..84
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        65..83
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    84..108
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             123..145
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
ORIGIN
        1 ggcgccggtg atgccggcca cgatgcgtcc ggcgtagagg atcgagatct cgatcccgcg
       61 aaattaatac gactcactat aggggaattg tgagcggata acaattcccc tctagaaata
      121 attttgttta actttaagaa ggagatatac catggacaat ctgctgaact actcctcacg
      181 cgcctcggca atcccgtccc tgctgtgcga cttctacaaa acctctcatc gtattatgta
      241 tccggaatgc tctcagatta tctatagtac ctttacgccg cgttcgaacg aacaggcacc
      301 gtacctgacc caagtggtta gcttcggctt ccaagctttc atcatcaaat acctgatcca
      361 ttacttcaac gataactttt tcagccgtga taaacacgac gtcgtgaccg aatatagtgc
      421 gtttattgaa aaaaccctgc agctggaaga cacgggcgaa catatcgcca aactgcacga
      481 actgggttac ctgccgattc gtatcaaagc aattccggaa ggtaaaaccg ttgctattaa
      541 agttccggtc atgaccatcg aaaatacgca ttctgatttc ttttggctga ccaactatct
      601 ggaaacgctg atcaatgtca gtctgtggca gccgatgacc agcgcgtcaa ttgcgtttgc
      661 ctaccgtacc gcactgatca aattcgctaa cgaaacgtgt gataatcagg aacatgtgcc
      721 gtttcaatcc cacgacttct caatgcgcgg catgagctct ctggaatcgg cggaaaccag
      781 cggcgccggt catctgacgt cctttctggg taccgatacg attccggcgc tgtcattcgt
      841 tgaagcctat tacggcagtt cctcactgat tggtaccagc atcccggcat cggaacatag
      901 cgttatgtcg agccacggcg tcgatgaact gagcacgttt cgctatctga tggctaaatt
      961 cccgcataac atgctgtcta ttgtca
//
