LOCUS       sequence-162976-         905 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses nadV from Shewanella oneidensis in Escherichia coli.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 905)
  AUTHORS   Marinescu GC, Popescu RG, Stoian G, Dinischiotu A
  TITLE     beta-nicotinamide mononucleotide (NMN) production in Escherichia 
            coli.
  JOURNAL   Sci Rep. 2018 Aug 16;8(1):12278. doi: 10.1038/s41598-018-30792-0.
  PUBMED    30115969
REFERENCE   2  (bases 1 to 905)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Rep."; 
            date: "2018-08-16"; volume: "8(1):12278. doi"; pages: " 
            10.1038/s41598-018-30792-0"
COMMENT     Sequence Label: pET28a-soNadV
FEATURES             Location/Qualifiers
     source          1..905
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     terminator      complement(49..96)
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     primer_bind     92..110
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     CDS             complement(163..180)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 tacagggcgc gtcccattcg ccaatccgga tatagttcct cctttcagca aaaaacccct
       61 caagacccgt ttagaggccc caaggggtta tgctagttat tgctcagcgg tggcagcagc
      121 caactcagct tcctttcggg ctttgttagc agccggatct cagtggtggt ggtggtggtg
      181 ctcgagttag cgacgggatg cggccaggcg ggcacggatg tcagccaggg agtattcggt
      241 cagcagttca ccatcagcga agaccggaac aaattcgcaa ttgttcacgt cttgccacga
      301 tgcctgatct ttcagataga attggccctg ttcatcttta aacacggcga ccagaccttt
      361 cgctgatttt ttggtgccgt catccgtttt cggatctttg aaaatttcgc ggtctttacc
      421 atcaactttc gcaaaggtag ctttcactgc gtagccgtcg gtatcacgcg tcacgtgctg
      481 ataggtgaac gaaccgatgc caaagacaat gttcgttgaa gcaaaacctt ttgctttcag
      541 gccggcacag atagcttctg cgcgttcgat ggtaatggag tcaccgtaaa tcaggccaac
      601 atgactatcc agcagtttat aacctttcgc cgtggtagaa ccgccaaaga catcccacag
      661 gcattcgatg gcacctttgt attccgggct accaatcggg gcctgcgggt caccacagat
      721 aattttaacc gggcagccgg tatccggacg aaaaaccact ttaccgtcgc gagccaggat
      781 acgatctttc agtgccaggg tgaattccgt catcacctgc caaaagtccc aagaatcgct
      841 aacgatactc acaatgccgg tcggatagat atcttcaatc aggcgacgaa acgtttccag
      901 ttcat
//
