LOCUS       sequence-162982-        1004 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  GCaMP6f expression in forebrain GABA-ergic interneurons under the 
            control of the mDlx enhancer element.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1004)
  AUTHORS   Dimidschstein J, Chen Q, Tremblay R, Rogers SL, Saldi GA, Guo L, Xu 
            Q, Liu R, Lu C, Chu J, Avery MC, Rashid MS, Baek M, Jacob AL, Smith 
            GB, Wilson DE, Kosche G, Kruglikov I, Rusielewicz T, Kotak VC, 
            Mowery TM, Anderson SA, Callaway EM, Dasen JS, Fitzpatrick D, 
            Fossati V, Long MA, Noggle S, Reynolds JH, Sanes DH, Rudy B, Feng G,
            Fishell G
  TITLE     A viral strategy for targeting and manipulating interneurons across 
            vertebrate species.
  JOURNAL   Nat Neurosci. 2016 Oct 31. doi: 10.1038/nn.4430.
  PUBMED    27798629
REFERENCE   2  (bases 1 to 1004)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Neurosci. 2016 Oct 31. doi: 10.1038/nn.4430."
COMMENT     Sequence Label: pAAV-mDlx-GCaMP6f-Fishell-2
FEATURES             Location/Qualifiers
     source          1..1004
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(195..251)
                     /codon_start=1
                     /product="calmodulin-binding peptide from avian smooth 
                     muscle myosin light chain kinase"
                     /label=calmodulin-binding peptide
                     /translation="RRKWNKTGHAVRAIGRLSS"
     regulatory      263..272
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             complement(join(279..293,294..302))
                     /codon_start=1
                     /product="Xpress(TM) epitope tag, including an enterokinase
                     recognition and cleavage site"
                     /label=Xpress(TM) tag
                     /translation="DLYDDDDK"
     CDS             complement(306..338)
                     /codon_start=1
                     /product="leader peptide from bacteriophage T7 gene 10"
                     /label=T7 tag (gene 10 leader)
                     /note="promotes efficient translation in E. coli"
                     /translation="MASMTGGQQMG"
     CDS             complement(342..359)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     regulatory      365..374
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(418..437)
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     intron          420..552
                     /label=chimeric intron
                     /note="chimera between introns from human beta-globin and 
                     immunoglobulin heavy chain genes"
ORIGIN
        1 gggtctttcg aaagtttgga ctgcacgctc aggtagtggt tgtcgggcag cagcacgggg
       61 ccgtcgccga tgggggtgtt ctgctggtag tggtaggcga gctgcacgcc gccgtcctcg
      121 atgttgtggc ggatcttgaa gttcgccttg atgccgttct tctgcttgtc ggccttgata
      181 tagacgttct cgagtgagct cagccgacct atagctctga ctgcgtgacc tgtcttattc
      241 cacttacgac gtgatgagtc gaccatggtg gcgagatcct tatcgtcatc gtcgtacaga
      301 tcccgaccca tttgctgtcc accagtcatg ctagccatac catgatgatg atgatgatga
      361 gaacccatgg tggcaccggt actagtctta agagctgtaa ttgaactggg agtggacacc
      421 tgtggagaga aaggcaaagt ggatgtcagt aagaccaata ggtgcctatc agaaacgcaa
      481 gagtcttctc tgtctcgaca agcccagttt ctattggtct ccttaaacct gtcttgtaac
      541 cttgatactt acctgcccag tgcctcacga ccaacttctg cagcttaagg cgccgcgctc
      601 tgcttctgga aggctgcgct cctcgacctg caggctagaa gcaaatgtaa gcaatagatg
      661 gctctgccct gacttttatg cccagcccgg gctgcaggaa ttcgatatca agcttcctac
      721 tgtgaaactt tgggttcgta gctccccagg atcaattctg aacaaagcct ccagctgcag
      781 tgccatccaa tttgaagcag acattgggga caatttaagg tttttatcca caagaaggtt
      841 tttttccatt ctcttaaatg cagccataat tagagtaatt tttcatgtag cccgctgatt
      901 acagcgtttt taccgtcaaa gataattacc tgtaattttc ttccactttt aatactaaaa
      961 agccatcttt atttagattc aggaacagga aaggcgaaac aaaa
//
