LOCUS       sequence-163000-        5586 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway Donor vector containing RBM10, part of the Target 
            Accelerator Plasmid Collection..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5586)
  AUTHORS   Berger AH, Brooks AN, Wu X, Shrestha Y, Chouinard C, Piccioni F, 
            Bagul M, Kamburov A, Imielinski M, Hogstrom L, Zhu C, Yang X, Pantel
            S, Sakai R, Watson J, Kaplan N, Campbell JD, Singh S, Root DE, 
            Narayan R, Natoli T, Lahr DL, Tirosh I, Tamayo P, Getz G, Wong B, 
            Doench J, Subramanian A, Golub TR, Meyerson M, Boehm JS
  TITLE     High-throughput Phenotyping of Lung Cancer Somatic Mutations.
  JOURNAL   Cancer Cell. 2016 Aug 8;30(2):214-28. doi: 
            10.1016/j.ccell.2016.06.022. Epub 2016 Jul 28.
  PUBMED    27478040
REFERENCE   2  (bases 1 to 5586)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.ccell.2016.06.022"; journalName: "Cancer Cell"; date: 
            "2016-08-8- 8"; volume: "30"; issue: "2"; pages: "214-28"
COMMENT     Sequence Label: pDONR223_RBM10_p.E4K
FEATURES             Location/Qualifiers
     source          1..5586
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     43..65
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     57..74
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     58..74
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    90..189
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             complement(929..946)
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     CDS             complement(1545..1556)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     protein_bind    complement(2986..3085)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(3102..3121)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(3103..3121)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(3126..3142)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3126..3142)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             3573..4364
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      4457..5045
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4946..4965
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     5199..5216
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(5375..5402)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(5494..5580)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
ORIGIN
        1 ggcagttccc tactctcgcg ttaacgctag catggatgtt ttcccagtca cgacgttgta
       61 aaacgacggc cagtcttaag ctcgggcccc aaataatgat tttattttga ctgatagtga
      121 cctgttcgtt gcaacaaatt gatgagcaat gcttttttat aatgccaact ttgtacaaaa
      181 aagttggcac catggagtat aaaagacgtg gtggtcgtgg tgacaggact ggccgctatg
      241 gagccactga ccgctcgcag gatgatggtg gggagaaccg cagccgagac cacgactacc
      301 gggacatgga ctaccgttca tatcctcgcg agtatggcag ccaggagggc aagcatgact
      361 atgacgactc atctgaggag cagagtgcgg aggattccta cgaggcctcc ccgggctccg
      421 agactcagcg taggcggcgg cggcggcaca ggcacagccc caccggcccg ccaggcttcc
      481 cccgagacgg cgactatcgg gaccaggact atcggaccga gcaaggggag gaggaggagg
      541 aggaggagga tgaggaggag gaggagaagg ccagtaacat cgtcatgctg aggatgctgc
      601 cacaggcagc cactgaggat gacatccgtg gccagctgca gtcgcacggc gtgcaagcac
      661 gggaggttcg gctgatgcgg aacaaatctt caggtcagag ccggggcttc gccttcgtcg
      721 agtttagtca cttgcaggac gctacacgat ggatggaagc caatcagcac tccctcaaca
      781 tcctgggcca gaaggtgtcg atgcactaca gtgaccccaa gcccaagatc aatgaggact
      841 ggctgtgcaa taagtgtggc gtccagaact tcaaacgccg agagaagtgc ttcaaatgtg
      901 gcgtgcccaa gtcagaggca gagcagaagc tgcccctcgg cacgaggctg gatcagcaga
      961 cactgccact gggtggccgg gagctgagcc agggcctgct tcccctgccg cagccctacc
     1021 aggcccaggg agtcctggcc tcccaagccc tgtcacaggg ctcggagcca agctcagaga
     1081 acgccaatga caccatcatt ttgcgcaacc tgaacccaca cagcaccatg gattccatcc
     1141 tgggggccct ggcaccctac gcggtgctgt cctcctccaa cgtgcgcgtc ataaaggaca
     1201 agcagaccca actgaaccgc ggctttgcct tcatccagct ctccaccatc gtggaggcag
     1261 cccagctgct gcagatcctg caggccctgc acccaccact cactatcgac ggcaagacca
     1321 tcaatgttga gtttgccaag ggttctaaga gggacatggc ctccaatgaa ggcagtcgca
     1381 tcagtgctgc ctctgtggcc agcactgcca ttgctgcggc ccagtgggcc atctcacagg
     1441 cctcccaagg tggggagggt acctgggcca cctccgagga gccgccggtc gactacagct
     1501 actaccaaca ggatgagggc tatggcaaca gccagggcac agagtcttcc ctctatgccc
     1561 atggctacct caagggcacc aagggccctg gcatcactgg aaccaaaggg gatcccactg
     1621 gagcaggtcc cgaggcctcc ctagagcctg gggccgactc tgtgtcgatg caggctttct
     1681 ctcgcgccca gcctggtgct gctcctggca tctaccaaca atcagccgag gcgagcagta
     1741 gccagggcac tgctgccaac agccagtcgt ataccatcat gtcacccgct gtgctcaaat
     1801 ctgagctcca gagccctacc catcctagtt ctgctctccc accggctacc agccccactg
     1861 cccaggaatc ctacagccag taccctgttc ccgacgtctc tacctaccag tacgatgaga
     1921 cctccggcta ctactatgac ccccagaccg gcctctacta tgaccccaac tcccagtatt
     1981 actacaatgc tcagagccag cagtacctgt actgggatgg ggagaggcgg acctatgttc
     2041 ccgccctgga gcagtcggcc gacggacata aggagacagg ggcaccctcg aaggagggca
     2101 aagagaagaa ggagaagcac aagaccaaga cagctcaaca gattgccaag gacatggaac
     2161 gctgggcccg cagtctcaac aaacaaaaag aaaacttcaa aaatagcttc cagcctatca
     2221 gctccctgcg agatgacgag aggcgggagt cagccactgc agatgctggc tatgccatcc
     2281 tcgagaagaa gggagcacta gccgagagac agcacaccag catggatctc ccgaaattgg
     2341 ccagtgacga ccgcccaagc cctccgcgag gactggtggc agcctacagc ggggagagtg
     2401 acagtgagga ggagcaggag cgtgggggcc ctgagcggga ggagaagctc accgactggc
     2461 agaagctggc ctgtctgctc tgccgacgcc agttccccag caaagaggcg ctcatccggc
     2521 accagcagct ctcagggctc cacaagcaaa accttgagat tcaccggcga gcccacttgt
     2581 cagaaaacga gctagaagca ctagagaaga atgacatgga gcaaatgaag taccgggacc
     2641 gtgcagctga acgcagagaa aagtatggca tccccgagcc gccagagccc aagaggagga
     2701 agtacggcgg catatccaca gcctctgtag acttcgatca gcctactcgg gacgggctgg
     2761 gcagtgacaa cattggcagt cggatgctgc aggccatggg ctggaaagag ggcagcggcc
     2821 tgggccgcaa gaagcagggc attgtaacgc ctatcgaggc ccaaacacgg gtgcggggct
     2881 ccggcctggg tgcacggggc agctcctacg gggtcacctc aaccgagtcc tacaaggaga
     2941 cactgcacaa gacaatggtg acccgcttca acgaggccca gtgattgcca actttcttgt
     3001 acaaagttgg cattataaga aagcattgct tatcaatttg ttgcaacgaa caggtcacta
     3061 tcagtcaaaa taaaatcatt atttgccatc cagctgatat cccctatagt gagtcgtatt
     3121 acatggtcat agctgtttcc tggcagctct ggcccgtgtc tcaaaatctc tgatgttaca
     3181 ttgcacaaga taaaaatata tcatcatgcc tcctctagac cagccaggac agaaatgcct
     3241 cgacttcgct gctacccaag gttgccgggt gacgcacacc gtggaaacgg atgaaggcac
     3301 gaacccagtg gacataagcc tgttcggttc gtaagctgta atgcaagtag cgtatgcgct
     3361 cacgcaactg gtccagaacc ttgaccgaac gcagcggtgg taacggcgca gtggcggttt
     3421 tcatggcttg ttatgactgt ttttttgggg tacagtctat gcctcgggca tccaagcagc
     3481 aagcgcgtta cgccgtgggt cgatgtttga tgttatggag cagcaacgat gttacgcagc
     3541 agggcagtcg ccctaaaaca aagttaaaca ttatgaggga agcggtgatc gccgaagtat
     3601 cgactcaact atcagaggta gttggcgtca tcgagcgcca tctcgaaccg acgttgctgg
     3661 ccgtacattt gtacggctcc gcagtggatg gcggcctgaa gccacacagt gatattgatt
     3721 tgctggttac ggtgaccgta aggcttgatg aaacaacgcg gcgagctttg atcaacgacc
     3781 ttttggaaac ttcggcttcc cctggagaga gcgagattct ccgcgctgta gaagtcacca
     3841 ttgttgtgca cgacgacatc attccgtggc gttatccagc taagcgcgaa ctgcaatttg
     3901 gagaatggca gcgcaatgac attcttgcag gtatcttcga gccagccacg atcgacattg
     3961 atctggctat cttgctgaca aaagcaagag aacatagcgt tgccttggta ggtccagcgg
     4021 cggaggaact ctttgatccg gttcctgaac aggatctatt tgaggcgcta aatgaaacct
     4081 taacgctatg gaactcgccg cccgactggg ctggcgatga gcgaaatgta gtgcttacgt
     4141 tgtcccgcat ttggtacagc gcagtaaccg gcaaaatcgc gccgaaggat gtcgctgccg
     4201 actgggcaat ggagcgcctg ccggcccagt atcagcccgt catacttgaa gctagacagg
     4261 cttatcttgg acaagaagaa gatcgcttgg cctcgcgcgc agatcagttg gaagaatttg
     4321 tccactacgt gaaaggcgag atcaccaagg tagtcggcaa ataaccctcg agccacccat
     4381 gaccaaaatc ccttaacgtg agttacgcgt cgttccactg agcgtcagac cccgtagaaa
     4441 agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa
     4501 aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc
     4561 cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgttcttcta gtgtagccgt
     4621 agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc
     4681 tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac
     4741 gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca
     4801 gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg
     4861 ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag
     4921 gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt
     4981 ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat
     5041 ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc
     5101 acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt
     5161 gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag
     5221 cggaagagcg cccaatacgc aaaccgcctc tccccgcgcg ttggccgatt cattaatgca
     5281 gctggcacga caggtttccc gactggaaag cgggcagtga gcgcaacgca attaatacgc
     5341 gtaccgctag ccaggaagag tttgtagaaa cgcaaaaagg ccatccgtca ggatggcctt
     5401 ctgcttagtt tgatgcctgg cagtttatgg cgggcgtcct gcccgccacc ctccgggccg
     5461 ttgcttcaca acgttcaaat ccgctcccgg cggatttgtc ctactcagga gagcgttcac
     5521 cgacaaacaa cagataaaac gaaaggccca gtcttccgac tgagcctttc gttttatttg
     5581 atgcct
//
