LOCUS       sequence-163023-       10630 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  mammalian expession of mCherry-LacR-PALB2.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10630)
  AUTHORS   Orthwein A, Noordermeer SM, Wilson MD, Landry S, Enchev RI, Sherker 
            A, Munro M, Pinder J, Salsman J, Dellaire G, Xia B, Peter M, 
            Durocher D
  TITLE     A mechanism for the suppression of homologous recombination in G1 
            cells.
  JOURNAL   Nature. 2015 Dec 9. doi: 10.1038/nature16142.
  PUBMED    26649820
REFERENCE   2  (bases 1 to 10630)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nature. 
            2015 Dec 9. doi: 10.1038/nature16142."
COMMENT     Sequence Label: pDEST-mCherry-LacR-PALB2
FEATURES             Location/Qualifiers
     source          1..10630
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(44..63)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        235..614
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        615..818
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     769..789
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     protein_bind    820..838
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    841..859
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     869..893
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             984..1691
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(1129..1147)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     1397..1416
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     complement(1735..1754)
                     /label=LacI-R
                     /note="LacI, reverse primer"
     CDS             2796..2816
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     protein_bind    2826..2850
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(6416..6540)
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(6582..6599)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    6588..6812
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(6945..6964)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     protein_bind    7096..7143
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     CDS             7151..8173
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="KKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRGY
                     VLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGDFSESLTYCISRRAQGVTLQDLPE
                     TELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQT
                     VMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMFG
                     DSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDGN
                     FDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAKE
                     "
     polyA_signal    8303..8424
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8340..8359)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8394..8413
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(8473..8489)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8473..8489)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(8486..8508)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    8497..8513
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(8521..8527,8528..8545,8546..8551))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8566..8587
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(8704..8721)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8875..9463)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8955..8974)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9634..10425,10426..10494))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     10257..10276
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(10495..10599)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
      421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
      781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ccctatcagt gatagagatc
      841 tccctatcag tgatagagat cgtcgacgag ctcgtttagt gaaccgtcag atcgcctgga
      901 gacgccatcc acgctgtttt gacctccata gaagacaccg ggaccgatcc agcctccgga
      961 ctctagcgtt taaacttaag cttatggtga gcaagggcga ggaggataac atggccatca
     1021 tcaaggagtt catgcgcttc aaggtgcaca tggagggctc cgtgaacggc cacgagttcg
     1081 agatcgaggg cgagggcgag ggccgcccct acgagggcac ccagaccgcc aagctgaagg
     1141 tgaccaaggg tggccccctg cccttcgcct gggacatcct gtcccctcag ttcatgtacg
     1201 gctccaaggc ctacgtgaag caccccgccg acatccccga ctacttgaag ctgtccttcc
     1261 ccgagggctt caagtgggag cgcgtgatga acttcgagga cggcggcgtg gtgaccgtga
     1321 cccaggactc ctccctgcag gacggcgagt tcatctacaa ggtgaagctg cgcggcacca
     1381 acttcccctc cgacggcccc gtaatgcaga agaagaccat gggctgggag gcctcctccg
     1441 agcggatgta ccccgaggac ggcgccctga agggcgagat caagcagagg ctgaagctga
     1501 aggacggcgg ccactacgac gctgaggtca agaccaccta caaggccaag aagcccgtgc
     1561 agctgcccgg cgcctacaac gtcaacatca agttggacat cacctcccac aacgaggact
     1621 acaccatcgt ggaacagtac gaacgcgccg agggccgcca ctccaccggc ggcatggacg
     1681 agctgtacaa gtccggactc agatctcgag ggatggtgaa accagtaacg ttatacgatg
     1741 tcgcagagta tgccggtgtc tcttatcaga ccgtttcccg cgtggtgaac caggccagcc
     1801 acgtttctgc gaaaacgcgg gaaaaagtgg aagcggcgat ggcggagctg aattacattc
     1861 ccaaccgcgt ggcacaacaa ctggcgggca aacagtcgtt gctgattggc gttgccacct
     1921 ccagtctggc cctgcacgcg ccgtcgcaaa ttgtcgcggc gattaaatct cgcgccgatc
     1981 aactgggtgc cagcgtggtg gtgtcgatgg tagaacgaag cggcgtcgaa gcctgtaaag
     2041 cggcggtgca caatcttctc gcgcaacgcg tcagtgggct gatcattaac tatccgctgg
     2101 atgaccagga tgccattgct gtggaagctg cctgcactaa tgttccggcg ttatttcttg
     2161 atgtctctga ccagacaccc atcaacagta ttattttctc ccatgaagac ggtacgcgac
     2221 tgggcgtgga gcatctggtc gcattgggtc accagcaaat cgcgctgtta gcgggcccat
     2281 taagttctgt ctcggcgcgt ctgcgtctgg ctggctggca taaatatctc actcgcaatc
     2341 aaattcagcc gatagcggaa cgggaaggcg actggagtgc catgtccggt tttcaacaaa
     2401 ccatgcaaat gctgaatgag ggcatcgttc ccactgcgat gctggttgcc aacgatcaga
     2461 tggcgctggg cgcaatgcgc gccattaccg agtccgggct gcgcgttggt gcggatatct
     2521 cggtagtggg atacgacgat accgaagaca gctcatgtta tatcccgccg ttaaccacca
     2581 tcaaacagga ttttcgcctg ctggggcaaa ccagcgtgga ccgcttgctg caactctctc
     2641 agggccaggc ggtgaagggc aatcagctgt tgcccgtctc actggtgaaa agaaaaacca
     2701 ccctggcgcc caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc
     2761 tggcacgaca ggtttcccga agcagcctga ggcctcctaa gaagaagagg aaggttggcg
     2821 cgcccacaag tttgtacaaa aaagcaggct ccaccatgga cgagcctccc gggaagcccc
     2881 tcagctgtga ggagaaggaa aagttaaagg agaaattagc attcttgaaa agggaataca
     2941 gcaagacact agcccgcctt cagcgtgccc aaagagctga aaagattaag cattctatta
     3001 agaaaacagt agaagaacaa gattgtttgt ctcagcagga tctctcaccg cagctaaaac
     3061 actcagaacc taaaaataaa atatgtgttt atgacaagtt acacatcaaa acccatcttg
     3121 atgaagaaac tggagaaaag acatctatca cacttgatgt tgggcctgag tcctttaacc
     3181 ctggagatgg cccaggagga ttacctatac aaagaacaga tgacacccaa gaacattttc
     3241 cccacagggt cagtgaccct agtggtgagc aaaagcagaa gctgccaagc agaagaaaga
     3301 agcagcagaa gaggacattt atttcacagg agagagactg tgtctttggc actgattcac
     3361 tcagattgtc tgggaaaaga ctaaaggaac aggaagaaat cagtagcaaa aatcctgcta
     3421 gatcaccagt aactgaaata agaactcacc ttttaagtct taaatctgaa cttccagatt
     3481 ctccagaacc agttacagaa attaatgaag acagtgtatt aattccacca actgcccaac
     3541 cagaaaaagg tgttgataca ttcctaagaa gacctaattt caccagggcg actacagttc
     3601 ctttacagac tctatcagat agcggtagta gtcagcacct tgaacacatt cctcctaaag
     3661 gtagcagtga acttactact cacgacctaa aaaacattag atttacttca cctgtaagtt
     3721 tggaggcaca aggcaaaaaa atgactgtct ctacagataa cctccttgta aataaagcta
     3781 taagtaaaag tggccaactg cccacaagtt ctaatttaga ggcaaatatt tcatgttctc
     3841 taaatgaact cacctacaat aacttaccag caaatgaaaa ccaaaactta aaagaacaaa
     3901 atcaaacaga gaaatcttta aaatctccca gtgacactct tgatggcagg aatgaaaatc
     3961 ttcaggaaag tgagattcta agtcaaccta agagtcttag cctggaagca acctctcctc
     4021 tttctgcaga aaaacattct tgcacagtgc ctgaaggcct tctgtttcct gcagaatatt
     4081 atgttagaac aacacgaagc atgtccaatt gccagaggaa agtagccgtg gaggctgtca
     4141 ttcagagtca tttggatgtc aagaaaaaag ggtttaaaaa taaaaataag gatgcaagta
     4201 aaaatttaaa cctttccaat gaggaaactg accaaagtga aattaggatg tctggcacat
     4261 gcacaggaca accaagttca agaacctctc agaaacttct ctcattaact aaagtcagct
     4321 ctcccgctgg gcccactgaa gataatgact tgtctaggaa ggcagttgcc caagcacctg
     4381 gtagaagata cacaggaaaa agaaaatcag cctgcacccc agcatcagat cattgtgaac
     4441 cacttttgcc aacttctagc ctgtcgattg ttaacaggtc caaggaagaa gtcacctcac
     4501 acaaatatca gcacgaaaaa ttatttattc aagtgaaagg gaagaaaagt cgtcatcaaa
     4561 aagaggattc cctttcttgg agtaatagtg cttatttatc cttggatgat gatgctttca
     4621 cggctccatt tcatagggat ggaatgctga gtttaaagca actactgtct tttctcagta
     4681 tcacagactt tcagttacct gatgaagact ttggacctct taagcttgaa aaagtgaagt
     4741 cctgctcaga aaaaccagtg gagccctttg agtcaaaaat gtttggagag agacatctta
     4801 aagagggaag ctgtattttt ccagaggaac tgagtcctaa acgcatggat acagaaatgg
     4861 aggacttaga agaggacctt attgttctac caggaaaatc acatcccaaa aggccaaact
     4921 cgcaaagcca gcatacaaag acgggccttt cttcatccat attactttat actcctttaa
     4981 atacggttgc gcctgatgat aatgacaggc ctaccacaga catgtgttca cctgctttcc
     5041 ccatcttagg tactactcca gcctttggcc ctcaaggctc ctatgaaaaa gcatctacag
     5101 aagttgctgg acgaacttgc tgcacacccc aacttgctca tttgaaagac tcagtctgtc
     5161 ttgccagtga tactaaacaa ttcgacagtt caggcagccc agcaaaacca cataccaccc
     5221 tgcaagtgtc aggcaggcaa ggacaaccta cctgtgactg tgactctgtc ccgccaggaa
     5281 cacctccacc cattgagtca ttcactttta aagaaaatca gctctgtaga aacacatgcc
     5341 aggagctgca taaacattcc gtcgaacaga ctgaaacagc agagcttcct gcttctgata
     5401 gcataaaccc aggcaaccta caattggttt cagagttaaa gaatccttca ggttcctgtt
     5461 ccgtagatgt gagtgccatg ttttgggaaa gagccggttg taaagagcca tgtatcataa
     5521 ctgcttgcga agatgtagtt tctctttgga aagctctgga tgcttggcag tgggaaaaac
     5581 tttatacctg gcacttcgca gaggttccag tattacagat agttccagtg cctgatgtgt
     5641 ataatctcgt gtgtgtagct ttgggaaatt tggaaatcag agagatcagg gcattgtttt
     5701 gttcctctga tgatgaaagt gaaaagcaag tactactgaa gtctggaaat ataaaagctg
     5761 tgcttggcct gacaaagagg aggctagtta gtagcagtgg gaccctttct gatcaacaag
     5821 tagaagtcat gacgtttgca gaagatggag gaggcaaaga aaaccaattt ttgatgcccc
     5881 ctgaggagac tatactaact tttgctgagg tccaagggat gcaagaagct ctgcttggta
     5941 ctactattat gaacaacatt gttatttgga atttaaaaac tggtcaactc ctgaaaaaga
     6001 tgcacattga tgattcttac caagcttcag tctgtcacaa agcctattct gaaatggggc
     6061 ttctctttat tgtcctgagt catccctgtg ccaaagagag tgagtcgttg cgaagccctg
     6121 tgtttcagct cattgtgatt aaccctaaga cgactctcag cgtgggtgtg atgctgtact
     6181 gtcttcctcc agggcaggct ggcaggttcc tggaaggtga cgtgaaagat cactgtgcag
     6241 cagcaatctt gacttctgga acaattgcca tttgggactt acttctcggt cagtgtactg
     6301 ccctcctccc acctgtctct gaccaacatt ggtcttttgt gaaatggtcg ggtacagact
     6361 ctcatttgct ggctggacaa aaagatggaa atatatttgt ataccactat tcataaatag
     6421 tgactggata tgttgtgttt tacagtatta tgtagtctgt tttttatgca aaatctaatt
     6481 taatatattg atatttatat cattttacgt ttctcgttca gctttcttgt acaaagtggt
     6541 gtgactcgag tctagagggc ccgtttaaac ccgctgatca gcctcgactg tgccttctag
     6601 ttgccagcca tctgttgttt gcccctcccc cgtgccttcc ttgaccctgg aaggtgccac
     6661 tcccactgtc ctttcctaat aaaatgagga aattgcatcg cattgtctga gtaggtgtca
     6721 ttctattctg gggggtgggg tggggcagga cagcaagggg gaggattggg aagacaatag
     6781 caggcatgct ggggatgcgg tgggctctat ggcttctgag gcggaaagaa ccagctgggg
     6841 ctctaggggg tatccccacg cgccctgtag cggcgcatta agcgcggcgg gtgtggtggt
     6901 tacgcgcagc gtgaccgcta cacttgccag cgccctagcg cccgctcctt tcgctttctt
     6961 cccttccttt ctcgccacgt tcgccggctt tccccgtcaa gctctaaatc gggggctccc
     7021 tttagggttc cgatttagtg ctttacggca cctcgacccc aaaaaacttg attagggtga
     7081 tggttcacgt acctagaagt tcctattccg aagttcctat tctctagaaa gtataggaac
     7141 ttccttggcc aaaaagcctg aactcaccgc gacgtctgtc gagaagtttc tgatcgaaaa
     7201 gttcgacagc gtctccgacc tgatgcagct ctcggagggc gaagaatctc gtgctttcag
     7261 cttcgatgta ggagggcgtg gatatgtcct gcgggtaaat agctgcgccg atggtttcta
     7321 caaagatcgt tatgtttatc ggcactttgc atcggccgcg ctcccgattc cggaagtgct
     7381 tgacattggg gatttcagcg agagcctgac ctattgcatc tcccgccgtg cacagggtgt
     7441 cacgttgcaa gacctgcctg aaaccgaact gcccgctgtt ctgcagccgg tcgcggaggc
     7501 catggatgcg atcgctgcgg ccgatcttag ccagacgagc gggttcggcc cattcggacc
     7561 gcaaggaatc ggtcaataca ctacatggcg tgatttcata tgcgcgattg ctgatcccca
     7621 tgtgtatcac tggcaaactg tgatggacga caccgtcagt gcgtccgtcg cgcaggctct
     7681 cgatgagctg atgctttggg ccgaggactg ccccgaagtc cggcacctcg tgcacgcgga
     7741 tttcggctcc aacaatgtcc tgacggacaa tggccgcata acagcggtca ttgactggag
     7801 cgaggcgatg ttcggggatt cccaatacga ggtcgccaac atcttcttct ggaggccgtg
     7861 gttggcttgt atggagcagc agacgcgcta cttcgagcgg aggcatccgg agcttgcagg
     7921 atcgccgcgg ctccgggcgt atatgctccg cattggtctt gaccaactct atcagagctt
     7981 ggttgacggc aatttcgatg atgcagcttg ggcgcagggt cgatgcgacg caatcgtccg
     8041 atccggagcc gggactgtcg ggcgtacaca aatcgcccgc agaagcgcgg ccgtctggac
     8101 cgatggctgt gtagaagtac tcgccgatag tggaaaccga cgccccagca ctcgtccgag
     8161 ggcaaaggaa tagcacgtac tacgagattt cgattccacc gccgccttct atgaaaggtt
     8221 gggcttcgga atcgttttcc gggacgccgg ctggatgatc ctccagcgcg gggatctcat
     8281 gctggagttc ttcgcccacc ccaacttgtt tattgcagct tataatggtt acaaataaag
     8341 caatagcatc acaaatttca caaataaagc atttttttca ctgcattcta gttgtggttt
     8401 gtccaaactc atcaatgtat cttatcatgt ctgtataccg tcgacctcta gctagagctt
     8461 ggcgtaatca tggtcatagc tgtttcctgt gtgaaattgt tatccgctca caattccaca
     8521 caacatacga gccggaagca taaagtgtaa agcctggggt gcctaatgag tgagctaact
     8581 cacattaatt gcgttgcgct cactgcccgc tttccagtcg ggaaacctgt cgtgccagct
     8641 gcattaatga atcggccaac gcgcggggag aggcggtttg cgtattgggc gctcttccgc
     8701 ttcctcgctc actgactcgc tgcgctcggt cgttcggctg cggcgagcgg tatcagctca
     8761 ctcaaaggcg gtaatacggt tatccacaga atcaggggat aacgcaggaa agaacatgtg
     8821 agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca
     8881 taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa
     8941 cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc
     9001 tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc
     9061 gctttctcat agctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct
     9121 gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg
     9181 tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag
     9241 gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta
     9301 cggctacact agaagaacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg
     9361 aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt
     9421 tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt
     9481 ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgag
     9541 attatcaaaa aggatcttca cctagatcct tttaaattaa aaatgaagtt ttaaatcaat
     9601 ctaaagtata tatgagtaaa cttggtctga cagttaccaa tgcttaatca gtgaggcacc
     9661 tatctcagcg atctgtctat ttcgttcatc catagttgcc tgactccccg tcgtgtagat
     9721 aactacgata cgggagggct taccatctgg ccccagtgct gcaatgatac cgcgagaccc
     9781 acgctcaccg gctccagatt tatcagcaat aaaccagcca gccggaaggg ccgagcgcag
     9841 aagtggtcct gcaactttat ccgcctccat ccagtctatt aattgttgcc gggaagctag
     9901 agtaagtagt tcgccagtta atagtttgcg caacgttgtt gccattgcta caggcatcgt
     9961 ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc ggttcccaac gatcaaggcg
    10021 agttacatga tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt
    10081 tgtcagaagt aagttggccg cagtgttatc actcatggtt atggcagcac tgcataattc
    10141 tcttactgtc atgccatccg taagatgctt ttctgtgact ggtgagtact caaccaagtc
    10201 attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa
    10261 taccgcgcca catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg
    10321 aaaactctca aggatcttac cgctgttgag atccagttcg atgtaaccca ctcgtgcacc
    10381 caactgatct tcagcatctt ttactttcac cagcgtttct gggtgagcaa aaacaggaag
    10441 gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa tgttgaatac tcatactctt
    10501 cctttttcaa tattattgaa gcatttatca gggttattgt ctcatgagcg gatacatatt
    10561 tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc
    10621 acctgacgtc
//
