LOCUS       sequence-163043-         949 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  This plasmid can be used as a donor for subcloning into Gateway 
            destination vectors..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 949)
  AUTHORS   Chymkowitch P, Nguea AP, Aanes H, Koehler CJ, Thiede B, Lorenz S, 
            Meza-Zepeda LA, Klungland A, Enserink JM
  TITLE     Sumoylation of Rap1 mediates the recruitment of TFIID to promote 
            transcription of ribosomal protein genes.
  JOURNAL   Genome Res. 2015 Jun;25(6):897-906. doi: 10.1101/gr.185793.114. Epub
            2015 Mar 23.
  PUBMED    25800674
REFERENCE   2  (bases 1 to 949)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1101/gr.185793.114"; journalName: "Genome Res"; date: "2015-06";
            volume: "25"; issue: "6"; pages: "897-906"
COMMENT     Sequence Label: pDONR221-rap1-K43-117-118R
FEATURES             Location/Qualifiers
     source          1..949
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    9..108
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
ORIGIN
        1 ctggatggca aataatgatt ttattttgac tgatagtgac ctgttcgttg caacaaattg
       61 ataagcaatg ctttcttata atgccaactt tgtacaagaa agctgggttc ataacaggtc
      121 cttctcaaaa aatcttttcc tcatttccat tctaccagtt ccatgctttt taaccagttt
      181 ccttatttgt tcttgatcat tgctttttag cgattcgtcg tcatcatgtg tccaaatacc
      241 aggaacgttg ggaggaggat taacattgtc cttaaacatg ttcaagaaat agcgaggaaa
      301 gaccatcaga tccccggata aacacgtcaa tatgctcgta ctgaaatttt tacgtatacc
      361 agtttcatcg caaagatcct gtaccagttt ttcagcctgt gatggctcat aatctcctga
      421 aataacttca tgaagtttat ccataaattg tctcgtactg ccaaaatgaa agaacaatgg
      481 caggaaaaga tctgttgttg caatttttgg tggaaagctt atggtatcag gatcaatatt
      541 atcatatata tcttcattcg aaaaatcgct tcttatagct tccgcaatct cgtgtggata
      601 ctcaaagggc aaactatttg aaatattgga tagatcattc tttaaactgg aaatgaaatt
      661 catggtatct tcgtccagta attcgttttc tggtatagcg taagagttgg aagcagcggc
      721 agcagcagcg gcagcagcat tagcggacgc tgcattggca gtaggctgaa catttctttg
      781 agaactataa tttcttgccc tcttggcggc agagttataa ttgccaggag tgggaacgcc
      841 aggcctcttg ggtctattgg tgaggttctt catcggttcc ggctctctat tctgagcttt
      901 ttcagcttca taataactaa tataatcatc aatgccgtaa gcgagaaga
//
