LOCUS       sequence-163055-       12366 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral vector for expressing HRP-MDGA1 in neurons.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12366)
  AUTHORS   Loh KH, Stawski PS, Draycott AS, Udeshi ND, Lehrman EK, Wilton DK, 
            Svinkina T, Deerinck TJ, Ellisman MH, Stevens B, Carr SA, Ting AY
  TITLE     Proteomic Analysis of Unbounded Cellular Compartments: Synaptic 
            Clefts.
  JOURNAL   Cell. 2016 Aug 25;166(5):1295-1307.e21. doi: 
            10.1016/j.cell.2016.07.041.
  PUBMED    27565350
REFERENCE   2  (bases 1 to 12366)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2016.07"; journalName: "Cell"; date: "2016-08-25- 
            25"; volume: "166"; issue: "5"; pages: "1295-1307.e21"
COMMENT     Sequence Label: FSW-HRP-V5-MDGA1
FEATURES             Location/Qualifiers
     source          1..12366
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(47..66)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        238..617
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        619..817
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     772..792
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             835..1015
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    1062..1187
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1680..1913
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2098..2142
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2440..2557
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2636..3083
                     /label=hSyn promoter
                     /note="human synapsin I promoter; confers neuron-specific 
                     expression (Kugler et al., 2003)"
     regulatory      3091..3100
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             4144..4185
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     misc_feature    7058..7646
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(7111..7131)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(7529..7540)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(7649..7665)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7650..7666)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             8171..8351
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(8377..8394)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    8383..8607
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      8653..9081
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8740..8759)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     8950..8971
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(9090..9110)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        9095..9424
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      9275..9410
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     9337..9356
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        9472..9519
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             9538..9912
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    10042..10163
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(10079..10098)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     10133..10152
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(10212..10228)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(10212..10228)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(10225..10247)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    10236..10252
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(10260..10266,10267..10284,10285..10290))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    10305..10326
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(10443..10460)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(10614..11202)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(10694..10713)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(11373..12164,12165..12233))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     11996..12015
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(12234..12338)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gtcgacggat cgggagatct cccgatcccc tatggtgcac tctcagtaca atctgctctg
       61 atgccgcata gttaagccag tatctgctcc ctgcttgtgt gttggaggtc gctgagtagt
      121 gcgcgagcaa aatttaagct acaacaaggc aaggcttgac cgacaattgc atgaagaatc
      181 tgcttagggt taggcgtttt gcgctgcttc gcgatgtacg ggccagatat acgcgttgac
      241 attgattatt gactagttat taatagtaat caattacggg gtcattagtt catagcccat
      301 atatggagtt ccgcgttaca taacttacgg taaatggccc gcctggctga ccgcccaacg
      361 acccccgccc attgacgtca ataatgacgt atgttcccat agtaacgcca atagggactt
      421 tccattgacg tcaatgggtg gagtatttac ggtaaactgc ccacttggca gtacatcaag
      481 tgtatcatat gccaagcacg ccccctattg acgtcaatga cggtaaatgg cccgcctggc
      541 attatgccca gtacatgacc ttatgggact ttcctacttg gcagtacatc tacgtattag
      601 tcatcgctat taccatggtg atgcggtttt ggcagtacat caatgggcgt ggatagcggt
      661 ttgactcacg gggatttcca agtctccacc ccattgacgt caatgggagt ttgttttggc
      721 accaaaatca acgggacttt ccaaaatgtc gtaacaactc cgccccattg acgcaaatgg
      781 gcggtaggcg tgtacggtgg gaggtctata taagcagcgc gttttgcctg tactgggtct
      841 ctctggttag accagatctg agcctgggag ctctctggct aactagggaa cccactgctt
      901 aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac
      961 tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtggc
     1021 gcccgaacag ggacttgaaa gcgaaaggga aaccagagga gctctctcga cgcaggactc
     1081 ggcttgctga agcgcgcacg gcaagaggcg aggggcggcg actggtgagt acgccaaaaa
     1141 ttttgactag cggaggctag aaggagagag atgggtgcga gagcgtcagt attaagcggg
     1201 ggagaattag atcgcgatgg gaaaaaattc ggttaaggcc agggggaaag aaaaaatata
     1261 aattaaaaca tatagtatgg gcaagcaggg agctagaacg attcgcagtt aatcctggcc
     1321 tgttagaaac atcagaaggc tgtagacaaa tactgggaca gctacaacca tcccttcaga
     1381 caggatcaga agaacttaga tcattatata atacagtagc aaccctctat tgtgtgcatc
     1441 aaaggataga gataaaagac accaaggaag ctttagacaa gatagaggaa gagcaaaaca
     1501 aaagtaagac caccgcacag caagcggccg ctgatcttca gacctggagg aggagatatg
     1561 agggacaatt ggagaagtga attatataaa tataaagtag taaaaattga accattagga
     1621 gtagcaccca ccaaggcaaa gagaagagtg gtgcagagag aaaaaagagc agtgggaata
     1681 ggagctttgt tccttgggtt cttgggagca gcaggaagca ctatgggcgc agcgtcaatg
     1741 acgctgacgg tacaggccag acaattattg tctggtatag tgcagcagca gaacaatttg
     1801 ctgagggcta ttgaggcgca acagcatctg ttgcaactca cagtctgggg catcaagcag
     1861 ctccaggcaa gaatcctggc tgtggaaaga tacctaaagg atcaacagct cctggggatt
     1921 tggggttgct ctggaaaact catttgcacc actgctgtgc cttggaatgc tagttggagt
     1981 aataaatctc tggaacagat ttggaatcac acgacctgga tggagtggga cagagaaatt
     2041 aacaattaca caagcttaat acactcctta attgaagaat cgcaaaacca gcaagaaaag
     2101 aatgaacaag aattattgga attagataaa tgggcaagtt tgtggaattg gtttaacata
     2161 acaaattggc tgtggtatat aaaattattc ataatgatag taggaggctt ggtaggttta
     2221 agaatagttt ttgctgtact ttctatagtg aatagagtta ggcagggata ttcaccatta
     2281 tcgtttcaga cccacctccc aaccccgagg ggacccgaca ggcccgaagg aatagaagaa
     2341 gaaggtggag agagagacag agacagatcc attcgattag tgaacggatc ggcactgcgt
     2401 gcgccaattc tgcagacaaa tggcagtatt catccacaat tttaaaagaa aaggggggat
     2461 tggggggtac agtgcagggg aaagaatagt agacataata gcaacagaca tacaaactaa
     2521 agaattacaa aaacaaatta caaaaattca aaattttcgg gtttattaca gggacagcag
     2581 agatccagtt tggttaatta ataccgccat gcatctgcag agggccctgc gtatgagtgc
     2641 aagtgggttt taggaccagg atgaggcggg gtgggggtgc ctacctgacg accgaccccg
     2701 acccactgga caagcaccca acccccattc cccaaattgc gcatccccta tcagagaggg
     2761 ggaggggaaa caggatgcgg cgaggcgcgt gcgcactgcc agcttcagca ccgcggacag
     2821 tgccttcgcc cccgcctggc ggcgcgcgcc accgccgcct cagcactgaa ggcgcgctga
     2881 cgtcactcgc cggtcccccg caaactcccc ttcccggcca ccttggtcgc gtccgcgccg
     2941 ccgccggccc agccggaccg caccacgcga ggcgcgagat aggggggcac gggcgcgacc
     3001 atctgcgctg cggcgccggc gactcagcgc tgcctcagtc tgcggtgggc agcggaggag
     3061 tcgtgtcgtg cctgagagcg cagtctagac gccaccatgg atttcctgct gctcggtctc
     3121 tgtctatact ggctgctgag gaggccctcg ggggtggtct tgtgtctgct gggggcctgc
     3181 tttcagatgc tgcccgccgc tagccagctt actccaactt tctacgataa ctcttgtcct
     3241 aacgtgagca acatcgtgcg agatactatc gtgaacgagt tgcggtctga tccgaggata
     3301 gccgcttcaa ttttgcggct gcatttccac gattgttttg taaacggctg tgatgcttcc
     3361 atcctcctcg acaataccac ctctttcagg actgaaaagg atgccttcgg taacgcgaat
     3421 agtgctagag ggtttcccgt gatcgataga atgaaagccg ctgtggagag cgcctgcccc
     3481 cggacagtta gttgtgcaga ccttctgacc atagcggccc agcaatccgt gacgctggcc
     3541 ggtggcccca gctggagggt ccccctggga aggagggact ctctgcaggc ttttctggac
     3601 ttggcaaacg caaacctgcc tgctcccttc ttcacccttc cccagctgaa ggattccttc
     3661 aggaatgtcg gcctgaaccg aagtagtgat ttggtggctc tgtccggagg ccacacattc
     3721 ggcaagaatc agtgcaggtt catcatggac agactgtata acttttccaa caccggcttg
     3781 cctgacccta ccctgaacac aacatacttg cagactctca gagggctgtg tccgctcaat
     3841 ggcaacttgt ctgcactggt tgactttgac ctgcgcaccc caaccatctt cgacaataag
     3901 tattacgtca atctggagga acagaagggc ctgattcagt ccgaccagga gctgttcagt
     3961 tccccaaatg ctaccgacac tattcctctt gtgcgcagct tcgctaatag cacacaaacc
     4021 tttttcaatg ctttcgtgga agctatggat cggatgggaa atataacgcc cctgacgggt
     4081 acgcagggac agataagact gaactgtagg gtcgtgaaca gcaactctgg ctcgggctcg
     4141 ggcggtaagc ccatccccaa ccccctgctg ggcctggaca gcaccaccgg tcactgccgg
     4201 ggacaaggag tctacgctcc agcccaggcg cagatcgtgc atgcgggcca ggcatgtgtg
     4261 gtgaaagagg acaatatcag cgagcgtgtc tacaccatcc gggaggggga caccctcatg
     4321 ctgcagtgcc ttgtaacagg gcaccctcga ccccaggtac ggtggaccaa gacggcaggt
     4381 agcgcctcgg acaagttcca ggagacatcg gtgttcaacg agacgctgcg catcgagcgt
     4441 attgcacgca cgcagggcgg ccgctactac tgcaaggctg agaacggcgt gggggtgccg
     4501 gccatcaagt ccatccgcgt ggacgtgcag tacctggatg agccaatgct gacggtgcac
     4561 cagacggtga gcgatgtgcg aggcaacttc taccaggaga agacggtgtt cctgcgctgt
     4621 actgtcaact ccaacccgcc tgcccgcttc atctggaagc ggggttccga taccctatcc
     4681 cacagccagg acaatggggt tgacatctat gagcccctct acactcaggg ggagaccaag
     4741 gtcctgaagc tgaagaacct gcggccccag gactatgcca gctacacctg ccaggtgtct
     4801 gtgcgtaacg tgtgcggcat cccagacaag gccatcacct tccggctcac caacaccacg
     4861 gcaccaccag ccctgaagct gtctgtgaac gaaactctgg tggtgaaccc tggggagaat
     4921 gtgacggtgc agtgtctgct gacaggcggt gatcccctcc cccagctgca gtggtcccat
     4981 gggcctggcc cactgcccct gggtgctctg gcccagggtg gcaccctcag catcccttca
     5041 gtgcaggccc gggactctgg ctactacaac tgcacagcca ccaacaatgt gggcaaccct
     5101 gccaagaaga ctgtcaacct gctggtgcga tccatgaaga acgctacatt ccagatcact
     5161 cctgacgtga tcaaagagag tgagaacatc cagctgggcc aggacctgaa gctatcgtgc
     5221 cacgtggatg cagtgcccca ggagaaggtg acctaccagt ggttcaagaa tggcaagccg
     5281 gcacgcatgt ccaagcggct gctggtgacc cgcaatgatc ctgagctgcc cgcagtcacc
     5341 agcagcctag agctcattga cctgcacttc agtgactatg gcacctacct gtgcatggct
     5401 tctttcccag gggcacccgt gcccgacctc agcgtcgagg tcaacatctc ctctgagaca
     5461 gtgccgccca ccatcagtgt gcccaagggt agggccgtgg tgaccgtgcg cgagggatcg
     5521 cctgccgagc tgcaatgcga ggtgcggggc aagccgcggc cgccagtgct ctggtcccgc
     5581 gtggacaagg aggctgcact gctgccctcg gggctgcccc tggaggagac tccggacggg
     5641 aagctgcggc tggagcgagt gagccgagac atgagcggga cctaccgctg ccagacggcc
     5701 cgctataatg gcttcaacgt gcgcccccgt gaggcccagg tgcagctgaa cgtgcagttc
     5761 ccgccggagg tggagcccag ttcccaggac gtgcgccagg cgctgggccg gcccgtgctc
     5821 ctgcgctgct cgctgctgcg aggcagcccc cagcgcatcg cctcggctgt gtggcgtttc
     5881 aaagggcagc tgctgccgcc gccgcctgtt gttcccgccg ccgccgaggc gccggatcac
     5941 gcggagctgc gcctcgacgc cgtaactcgc gacagcagcg gcagctacga gtgcagcgtc
     6001 tccaacgatg tgggctcggc tgcctgcctc ttccaggtct ccgccaaagc ctacagcccg
     6061 gagttttact tcgacacccc caaccccacc cgcagccaca agctgtccaa gaactactcc
     6121 tacgtgctgc agtggactca gagggagccc gacgctgtcg accctgtgct caactacaga
     6181 ctcagcatcc gccagttgaa ccagcacaat gcggtggtca aggccatccc ggtccggcgt
     6241 gtggagaagg ggcagctgct ggagtacatc ctgaccgatc tccgtgtgcc ccacagctat
     6301 gaggtccgcc tcacacccta taccaccttc ggggctggtg acatggcctc ccgcatcatc
     6361 cactacacag agcccatcaa ctctccgaac ctttcagaca acacctgcca ctttgaggat
     6421 gagaagatct gtggctatac ccaggacctg acagacaact ttgactggac gcggcagaat
     6481 gccctcaccc agaaccccaa acgctccccc aacactggtc cccccaccga cataagtggc
     6541 acccctgagg gctactacat gttcatcgag acatcgaggc ctcgggagct gggggaccgt
     6601 gcaaggttag tgagtcccct ctacaatgcc agcgccaagt tctactgtgt ctccttcttc
     6661 taccacatgt acgggaaaca catcggctcc ctcaacctcc tggtgcggtc ccggaacaaa
     6721 ggggctctgg acacgcacgc ctggtctctc agtggcaata agggcaatgt gtggcagcag
     6781 gcccatgtgc ccatcagccc cagtgggccc ttccagatta tttttgaggg ggttcgaggc
     6841 ccgggctacc tgggggatat tgccatagat gacgtcacac tgaagaaggg ggagtgtccc
     6901 cggaagcaga cggatcccaa taaagtggtg gtgatgccgg gcagtggagc cccctgccag
     6961 tccagcccac agctgtgggg gcccatggcc atcttcctct tggcgttgca gagatgaggc
     7021 gcgcccctgc agggaattcg atatcaagct tatcgataat caacctctgg attacaaaat
     7081 ttgtgaaaga ttgactggta ttcttaacta tgttgctcct tttacgctat gtggatacgc
     7141 tgctttaatg cctttgtatc atgctattgc ttcccgtatg gctttcattt tctcctcctt
     7201 gtataaatcc tggttgctgt ctctttatga ggagttgtgg cccgttgtca ggcaacgtgg
     7261 cgtggtgtgc actgtgtttg ctgacgcaac ccccactggt tggggcattg ccaccacctg
     7321 tcagctcctt tccgggactt tcgctttccc cctccctatt gccacggcgg aactcatcgc
     7381 cgcctgcctt gcccgctgct ggacaggggc tcggctgttg ggcactgaca attccgtggt
     7441 gttgtcgggg aaatcatcgt cctttccttg gctgctcgcc tgtgttgcca cctggattct
     7501 gcgcgggacg tccttctgct acgtcccttc ggccctcaat ccagcggacc ttccttcccg
     7561 cggcctgctg ccggctctgc ggcctcttcc gcgtcttcgc cttcgccctc agacgagtcg
     7621 gatctccctt tgggccgcct ccccgcatcg ataccgtcga cctcgagacc tagaaaaaca
     7681 tggagcaatc acaagtagca atacagcagc taccaatgct gattgtgcct ggctagaagc
     7741 acaagaggag gaggaggtgg gttttccagt cacacctcag gtacctttaa gaccaatgac
     7801 ttacaaggca gctgtagatc ttagccactt tttaaaagaa aaggggggac tggaagggct
     7861 aattcactcc caacgaagac aagatatcct tgatctgtgg atctaccaca cacaaggcta
     7921 cttccctgat tggcagaact acacaccagg gccagggatc agatatccac tgacctttgg
     7981 atggtgctac aagctagtac cagttgagca agagaaggta gaagaagcca atgaaggaga
     8041 gaacacccgc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg agagagaagt
     8101 attagagtgg aggtttgaca gccgcctagc atttcatcac atggcccgag agctgcatcc
     8161 ggactgtact gggtctctct ggttagacca gatctgagcc tgggagctct ctggctaact
     8221 agggaaccca ctgcttaagc ctcaataaag cttgccttga gtgcttcaag tagtgtgtgc
     8281 ccgtctgttg tgtgactctg gtaactagag atccctcaga cccttttagt cagtgtggaa
     8341 aatctctagc agggcccgtt taaacccgct gatcagcctc gactgtgcct tctagttgcc
     8401 agccatctgt tgtttgcccc tcccccgtgc cttccttgac cctggaaggt gccactccca
     8461 ctgtcctttc ctaataaaat gaggaaattg catcgcattg tctgagtagg tgtcattcta
     8521 ttctgggggg tggggtgggg caggacagca agggggagga ttgggaagac aatagcaggc
     8581 atgctgggga tgcggcgggc tctatggctt ctgaggcgga aagaaccagc tggggctcta
     8641 gggggtatcc ccacgcgccc tgtagcggcg cattaagcgc ggcgggtgtg gtggttacgc
     8701 gcagcgtgac cgctacactt gccagcgccc tagcgcccgc tcctttcgct ttcttccctt
     8761 cctttctcgc cacgttcgcc ggctttcccc gtcaagctct aaatcggggg ctccctttag
     8821 ggttccgatt tagtgcttta cggcacctcg accccaaaaa acttgattag ggtgatggtt
     8881 cacgtagtgg gccatcgccc tgatagacgg tttttcgccc tttgacgttg gagtccacgt
     8941 tctttaatag tggactcttg ttccaaactg gaacaacact caaccctatc tcggtctatt
     9001 cttttgattt ataagggatt ttgccgattt cggcctattg gttaaaaaat gagctgattt
     9061 aacaaaaatt taacgcgaat taattctgtg gaatgtgtgt cagttagggt gtggaaagtc
     9121 cccaggctcc ccagcaggca gaagtatgca aagcatgcat ctcaattagt cagcaaccag
     9181 gtgtggaaag tccccaggct ccccagcagg cagaagtatg caaagcatgc atctcaatta
     9241 gtcagcaacc atagtcccgc ccctaactcc gcccatcccg cccctaactc cgcccagttc
     9301 cgcccattct ccgccccatg gctgactaat tttttttatt tatgcagagg ccgaggccgc
     9361 ctctgcctct gagctattcc agaagtagtg aggaggcttt tttggaggcc taggcttttg
     9421 caaaaagctc ccgggagctt gtatatccat tttcggatct gatcagcacg tgttgacaat
     9481 taatcatcgg catagtatat cggcatagta taatacgaca aggtgaggaa ctaaaccatg
     9541 gccaagttga ccagtgccgt tccggtgctc accgcgcgcg acgtcgccgg agcggtcgag
     9601 ttctggaccg accggctcgg gttctcccgg gacttcgtgg aggacgactt cgccggtgtg
     9661 gtccgggacg acgtgaccct gttcatcagc gcggtccagg accaggtggt gccggacaac
     9721 accctggcct gggtgtgggt gcgcggcctg gacgagctgt acgccgagtg gtcggaggtc
     9781 gtgtccacga acttccggga cgcctccggg ccggccatga ccgagatcgg cgagcagccg
     9841 tgggggcggg agttcgccct gcgcgacccg gccggcaact gcgtgcactt cgtggccgag
     9901 gagcaggact gacacgtgct acgagatttc gattccaccg ccgccttcta tgaaaggttg
     9961 ggcttcggaa tcgttttccg ggacgccggc tggatgatcc tccagcgcgg ggatctcatg
    10021 ctggagttct tcgcccaccc caacttgttt attgcagctt ataatggtta caaataaagc
    10081 aatagcatca caaatttcac aaataaagca tttttttcac tgcattctag ttgtggtttg
    10141 tccaaactca tcaatgtatc ttatcatgtc tgtataccgt cgacctctag ctagagcttg
    10201 gcgtaatcat ggtcatagct gtttcctgtg tgaaattgtt atccgctcac aattccacac
    10261 aacatacgag ccggaagcat aaagtgtaaa gcctggggtg cctaatgagt gagctaactc
    10321 acattaattg cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc gtgccagctg
    10381 cattaatgaa tcggccaacg cgcggggaga ggcggtttgc gtattgggcg ctcttccgct
    10441 tcctcgctca ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt atcagctcac
    10501 tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa gaacatgtga
    10561 gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat
    10621 aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac
    10681 ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct
    10741 gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg
    10801 ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg
    10861 ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt
    10921 cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg
    10981 attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac
    11041 ggctacacta gaagaacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga
    11101 aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt
    11161 gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt
    11221 tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgaga
    11281 ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa aatgaagttt taaatcaatc
    11341 taaagtatat atgagtaaac ttggtctgac agttaccaat gcttaatcag tgaggcacct
    11401 atctcagcga tctgtctatt tcgttcatcc atagttgcct gactccccgt cgtgtagata
    11461 actacgatac gggagggctt accatctggc cccagtgctg caatgatacc gcgagaccca
    11521 cgctcaccgg ctccagattt atcagcaata aaccagccag ccggaagggc cgagcgcaga
    11581 agtggtcctg caactttatc cgcctccatc cagtctatta attgttgccg ggaagctaga
    11641 gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg ccattgctac aggcatcgtg
    11701 gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg gttcccaacg atcaaggcga
    11761 gttacatgat cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt
    11821 gtcagaagta agttggccgc agtgttatca ctcatggtta tggcagcact gcataattct
    11881 cttactgtca tgccatccgt aagatgcttt tctgtgactg gtgagtactc aaccaagtca
    11941 ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat acgggataat
    12001 accgcgccac atagcagaac tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga
    12061 aaactctcaa ggatcttacc gctgttgaga tccagttcga tgtaacccac tcgtgcaccc
    12121 aactgatctt cagcatcttt tactttcacc agcgtttctg ggtgagcaaa aacaggaagg
    12181 caaaatgccg caaaaaaggg aataagggcg acacggaaat gttgaatact catactcttc
    12241 ctttttcaat attattgaag catttatcag ggttattgtc tcatgagcgg atacatattt
    12301 gaatgtattt agaaaaataa acaaataggg gttccgcgca catttccccg aaaagtgcca
    12361 cctgac
//
