LOCUS       sequence-163062-         978 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Can be used to produce inducible SIV-derived vector. Cells tranduced
            by lentiviral particles will overexpress GFP upon activation of the 
            tetO promoter. .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 978)
  AUTHORS   Mangeot PE, Dollet S, Girard M, Ciancia C, Joly S, Peschanski M, 
            Lotteau V
  TITLE     Protein transfer into human cells by VSV-G-induced nanovesicles.
  JOURNAL   Mol Ther. 2011 Sep;19(9):1656-66. doi: 10.1038/mt.2011.138. Epub 
            2011 Jul 12.
  PUBMED    21750535
REFERENCE   2  (bases 1 to 978)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/mt.2011.138"; journalName: "Mol Ther"; date: "2011-09"; 
            volume: "19"; issue: "9"; pages: "1656-66"
COMMENT     Sequence Label: GAE teo-GFP
FEATURES             Location/Qualifiers
     source          1..978
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(120..144)
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     promoter        complement(141..179)
                     /label=minimal CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     protein_bind    join(212..230,231..253,254..272,273..295,296..314,315..337,
                     338..356,357..379,380..398,399..421,422..440,441..463,
                     464..482)
                     /label=tetracycline response element
                     /bound_moiety="tetracycline repressor TetR"
                     /note="contains seven copies of the tetracycline operator 
                     tetO"
ORIGIN
        1 gctcctcgcc cttgctcacc atactggtgg cgaccggtag cttagccggg gccgcggagg
       61 ctggatcggt cccggtgtct tctatggagg tcaaaacagc gtggatggcg tctccaggcg
      121 atctgacggt tcactaaacg agctctgctt atataggcct cccaccgtac acgcctacct
      181 cgacccgggt accgagctcg actttcactt ttctctatca ctgataggga gtggtaaact
      241 cgactttcac ttttctctat cactgatagg gagtggtaaa ctcgactttc acttttctct
      301 atcactgata gggagtggta aactcgactt tcacttttct ctatcactga tagggagtgg
      361 taaactcgac tttcactttt ctctatcact gatagggagt ggtaaactcg actttcactt
      421 ttctctatca ctgataggga gtggtaaact cgactttcac ttttctctat cactgatagg
      481 gagtggtaaa ctcgtggacg aaagggatcg ataataggct aagggtactg ttgggtctgc
      541 gtatcatgga aataagaggg tggggaagag aacactggcc tatacccctg ccttaactta
      601 gctagcattt gtactatata gatcactatt cttaacagta ttactcctac aactacataa
      661 attccatatt gtatatactt tatccaagaa gcaaggtcaa accaattgcc aaacacatcc
      721 cagctattca acttttgtaa ttcatacatg ttcttctctt gttgaatttg tgcctcttct
      781 aggagggctg ttatattttc ctccaagaag tcaacctttc gctcccactc ttgccaagta
      841 tcattgttcc agtctggtgt tagacttgca tttggccatg gtacagtagt gtggcagact
      901 tgtctaaacg cacatcccca agcatttagc tgcgcctggt cctttaagta cttctcgatg
      961 gcagtgaccc tagtctgg
//
