LOCUS       sequence-163068-         850 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses Mst1 SARAH domain for interaction studies..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 850)
  AUTHORS   Rawat SJ, Araiza-Olivera D, Arias-Romero LE, Villamar-Cruz O, 
            Prudnikova TY, Roder H, Chernoff J
  TITLE     H-ras Inhibits the Hippo Pathway by Promoting Mst1/Mst2 
            Heterodimerization.
  JOURNAL   Curr Biol. 2016 Jun 20;26(12):1556-63. doi: 
            10.1016/j.cub.2016.04.027. Epub 2016 May 26.
  PUBMED    27238285
REFERENCE   2  (bases 1 to 850)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cub.2016.04.027"; journalName: "Curr Biol"; date: 
            "2016-06-20- 20"; volume: "26"; issue: "12"; pages: "1556-63"
COMMENT     Sequence Label: pGEX4T-Mst1 SARAH
FEATURES             Location/Qualifiers
     source          1..850
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        join(49..54,55..70,71..77)
                     /label=tac promoter
                     /note="strong E. coli promoter; hybrid between the trp and 
                     lac UV5 promoters"
     protein_bind    85..101
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     90..112
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     CDS             124..777
                     /codon_start=1
                     /product="glutathione S-transferase from Schistosoma 
                     japonicum"
                     /label=GST
                     /translation="MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKK
                     FELGLEFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVLDIRY
                     GVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTHPDFMLYDALDVVL
                     YMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIAWPLQGWQATFGGGDHPPK"
     primer_bind     735..757
                     /label=pGEX 5'
                     /note="pGEX vectors, Glutathione-S-transferase, forward 
                     primer"
     CDS             784..807
                     /codon_start=1
                     /product="recognition and cleavage site for human 
                     rhinovirus 3C and PreScission proteases"
                     /label=HRV 3C site
                     /translation="LEVLFQGP"
ORIGIN
        1 tttgcgccga catcataacg gttctggcaa atattctgaa atgagctgtt gacaattaat
       61 catcggctcg tataatgtgt ggaattgtga gcggataaca atttcacaca ggaaacagta
      121 ttcatgtccc ctatactagg ttattggaaa attaagggcc ttgtgcaacc cactcgactt
      181 cttttggaat atcttgaaga aaaatatgaa gagcatttgt atgagcgcga tgaaggtgat
      241 aaatggcgaa acaaaaagtt tgaattgggt ttggagtttc ccaatcttcc ttattatatt
      301 gatggtgatg ttaaattaac acagtctatg gccatcatac gttatatagc tgacaagcac
      361 aacatgttgg gtggttgtcc aaaagagcgt gcagagattt caatgcttga aggagcggtt
      421 ttggatatta gatacggtgt ttcgagaatt gcatatagta aagactttga aactctcaaa
      481 gttgattttc ttagcaagct acctgaaatg ctgaaaatgt tcgaagatcg tttatgtcat
      541 aaaacatatt taaatggtga tcatgtaacc catcctgact tcatgttgta tgacgctctt
      601 gatgttgttt tatacatgga cccaatgtgc ctggatgcgt tcccaaaatt agtttgtttt
      661 aaaaaacgta ttgaagctat cccacaaatt gataagtact tgaaatccag caagtatata
      721 gcatggcctt tgcagggctg gcaagccacg tttggtggtg gcgaccatcc tccaaaatcg
      781 gatctggaag ttctgttcca ggggcccctg ggatccgact acgagtttct taagagttgg
      841 acagtggagg
//
