LOCUS       sequence-163069-         960 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Cre recombinase-dependent GFP expression in forebrain GABA-ergic 
            interneurons under the control of the hDlx enhancer element.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 960)
  AUTHORS   Dimidschstein J, Chen Q, Tremblay R, Rogers SL, Saldi GA, Guo L, Xu 
            Q, Liu R, Lu C, Chu J, Avery MC, Rashid MS, Baek M, Jacob AL, Smith 
            GB, Wilson DE, Kosche G, Kruglikov I, Rusielewicz T, Kotak VC, 
            Mowery TM, Anderson SA, Callaway EM, Dasen JS, Fitzpatrick D, 
            Fossati V, Long MA, Noggle S, Reynolds JH, Sanes DH, Rudy B, Feng G,
            Fishell G
  TITLE     A viral strategy for targeting and manipulating interneurons across 
            vertebrate species.
  JOURNAL   Nat Neurosci. 2016 Oct 31. doi: 10.1038/nn.4430.
  PUBMED    27798629
REFERENCE   2  (bases 1 to 960)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Neurosci. 2016 Oct 31. doi: 10.1038/nn.4430."
COMMENT     Sequence Label: pAAV-hDlx-Flex-GFP-Fishell_6
FEATURES             Location/Qualifiers
     source          1..960
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    9..42
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     protein_bind    93..126
                     /label=lox2272
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (AAGTATCC) (Shaw et al., 2021). lox2272 sites are 
                     compatible with each other, but incompatible with loxP or 
                     loxN sites (Lee and Saito, 1988)."
     primer_bind     complement(174..193)
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     intron          176..308
                     /label=chimeric intron
                     /note="chimera between introns from human beta-globin and 
                     immunoglobulin heavy chain genes"
ORIGIN
        1 ggcgcgccat aacttcgtat aatgtatgct atacgaagtt attaagaggt ttcatattgc
       61 taatagcagc tacaatccag ctaccattct gcataacttc gtataaagta tcctatacga
      121 agttattccg gagtcgacct ttcttaagag ctgtaattga actgggagtg gacacctgtg
      181 gagagaaagg caaagtggat gtcagtaaga ccaataggtg cctatcagaa acgcaagagt
      241 cttctctgtc tcgacaagcc cagtttctat tggtctcctt aaacctgtct tgtaaccttg
      301 atacttacct gcccagtgcc tcacgaccaa cttctgcagc ttaaggcgcc gcgctctgct
      361 tctggaaggc tgcgctcctc gacctgcagg ctagaagcaa atgtaagcaa tagatggctc
      421 tgccctgact tttatgccca gcccgggctg caggaattcg atatcaagct tcctactgtg
      481 aaactttggg ttcgtagctc cccaggatca attctgaaca aagcctccag ctgcagtgcc
      541 atccaatttg aagcagacat tggggacaat ttaaggtttt tatccacaag aaggtttttt
      601 tccattctct taaatgcagc cataattaga gtaatttttc atgtagcccg ctgattacag
      661 cgtttttacc gtcaaagata attacctgta attttcttcc acttttaata ctaaaaagcc
      721 atctttattt agattcagga acaggaaagg cgaaacaaaa gagggaaatt attctgttat
      781 tcatacacaa attgcagaga cgtaggacct aaaattgaaa attaaccaaa attataatgc
      841 tgaaaagaat ggaagaggct gcagaagtac agctggtagt ggggctttgc tgaattattc
      901 aaattacgtt agggagaatg ctaccataca tcgaaaccaa cactaatttt tcgttaaaaa
//
