LOCUS       sequence-163078-        3776 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  reporter of GAPDH expression.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3776)
  AUTHORS   Cho W, Hagemann TL, Johnson DA, Johnson JA, Messing A
  TITLE     Dual transgenic reporter mice as a tool for monitoring expression of
            glial fibrillary acidic protein.
  JOURNAL   J Neurochem. 2009 Jul;110(1):343-51. Epub 2009 May 5.
  PUBMED    19457099
REFERENCE   2  (bases 1 to 3776)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J 
            Neurochem."; date: "2009-07"; volume: "110(1)"; pages: "343-51. Epub
            2009 May 5"
COMMENT     Sequence Label: hGAPDH-rLuc
FEATURES             Location/Qualifiers
     source          1..3776
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     intron          560..692
                     /label=chimeric intron
                     /note="chimera between introns from human beta-globin and 
                     immunoglobulin heavy chain genes"
     primer_bind     675..694
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     promoter        737..755
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      759..768
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             765..1700
                     /codon_start=1
                     /product="Renilla luciferase"
                     /label=hRluc
                     /note="human codon-optimized"
                     /translation="MASKVYDPEQRKRMITGPQWWARCKQMNVLDSFINYYDSEKHAEN
                     AVIFLHGNAASSYLWRHVVPHIEPVARCIIPDLIGMGKSGKSGNGSYRLLDHYKYLTAW
                     FELLNLPKKIIFVGHDWGACLAFHYSYEHQDKIKAIVHAESVVDVIESWDEWPDIEEDI
                     ALIKSEEGEKMVLENNFFVETMLPSKIMRKLEPEEFAAYLEPFKEKGEVRRPTLSWPRE
                     IPLVKGGKPDVVQIVRNYNAYLRASDDLPKMFIESDPGFFSNAIVEGAKKFPNTEFVKV
                     KGLHFSQEDAPDEMGKYIKSFVERVLKNEQ"
     polyA_signal    1731..1852
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(1742..1761)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     1796..1815
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     promoter        1985..2089
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(2090..2158,2159..2950)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(2308..2327)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      3121..3709
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3610..3629
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 agatctcgag gagaagttcc ccaactttcc cgcctctcag cctttgaaag aaagaaaggg
       61 gagggggcag gccgcgtgca gccgcgagcg gtgctgggct ccggctccaa ttccccatct
      121 cagtcgttcc caaagtcctc ctgtttcatc caagcgtgta agggtccccg tccttgactc
      181 cctagtgtcc tgctgcccac agtccagtcc tgggaaccag caccgatcac ctcccatcgg
      241 gccaatctca gtcccttccc ccctacgtcg gggcccacac gctcggtgcg tgcccagttg
      301 aaccaggcgg ctgcggaaaa aaaaaagcgg ggagaaagta gggcccggct actagcggtt
      361 ttacgggcgc acgtagctca ggcctcaaga ccttgggctg ggactggctg agcctggcgg
      421 gaggcggggt ccgagtcacc gcctgccgcc gcgcccccgg tttctataaa ttgagcccgc
      481 agcctcccgc ttcgctctct gctcctcctg ttggaattca cccgggtact gcagaagttg
      541 gtcgtgaggc actgggcagg taagtatcaa ggttacaaga caggtttaag gagaccaata
      601 gaaactgggc ttgtcgagac agagaagact cttgcgtttc tgataggcac ctattggtct
      661 tactgacatc cactttgcct ttctctccac aggtgtccac tcccagttca attacagctc
      721 ttaaggctag agtacttaat acgactcact ataggctagc caccatggct tccaaggtgt
      781 acgaccccga gcaacgcaaa cgcatgatca ctgggcctca gtggtgggct cgctgcaagc
      841 aaatgaacgt gctggactcc ttcatcaact actatgattc cgagaagcac gccgagaacg
      901 ccgtgatttt tctgcatggt aacgctgcct ccagctacct gtggaggcac gtcgtgcctc
      961 acatcgagcc cgtggctaga tgcatcatcc ctgatctgat cggaatgggt aagtccggca
     1021 agagcgggaa tggctcatat cgcctcctgg atcactacaa gtacctcacc gcttggttcg
     1081 agctgctgaa ccttccaaag aaaatcatct ttgtgggcca cgactggggg gcttgtctgg
     1141 cctttcacta ctcctacgag caccaagaca agatcaaggc catcgtccat gctgagagtg
     1201 tcgtggacgt gatcgagtcc tgggacgagt ggcctgacat cgaggaggat atcgccctga
     1261 tcaagagcga agagggcgag aaaatggtgc ttgagaataa cttcttcgtc gagaccatgc
     1321 tcccaagcaa gatcatgcgg aaactggagc ctgaggagtt cgctgcctac ctggagccat
     1381 tcaaggagaa gggcgaggtt agacggccta ccctctcctg gcctcgcgag atccctctcg
     1441 ttaagggagg caagcccgac gtcgtccaga ttgtccgcaa ctacaacgcc taccttcggg
     1501 ccagcgacga tctgcctaag atgttcatcg agtccgaccc tgggttcttt tccaacgcta
     1561 ttgtcgaggg agctaagaag ttccctaaca ccgagttcgt gaaggtgaag ggcctccact
     1621 tcagccagga ggacgctcca gatgaaatgg gtaagtacat caagagcttc gtggagcgcg
     1681 tgctgaagaa cgagcagtaa ttctagagcg gccgcttcga gcagacatga taagatacat
     1741 tgatgagttt ggacaaacca caactagaat gcagtgaaaa aaatgcttta tttgtgaaat
     1801 ttgtgatgct attgctttat ttgtaaccat tataagctgc aataaacaag ttaacaacaa
     1861 caattgcatt cattttatgt ttcaggttca gggggaggtg tgggaggttt tttaaagcaa
     1921 gtaaaacctc tacaaatgtg gtaaaatcga taaggatcca ggtggcactt ttcggggaaa
     1981 tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat
     2041 gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta tgagtattca
     2101 acatttccgt gtcgccctta ttcccttttt tgcggcattt tgccttcctg tttttgctca
     2161 cccagaaacg ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac gagtgggtta
     2221 catcgaactg gatctcaaca gcggtaagat ccttgagagt tttcgccccg aagaacgttt
     2281 tccaatgatg agcactttta aagttctgct atgtggcgcg gtattatccc gtattgacgc
     2341 cgggcaagag caactcggtc gccgcataca ctattctcag aatgacttgg ttgagtactc
     2401 accagtcaca gaaaagcatc ttacggatgg catgacagta agagaattat gcagtgctgc
     2461 cataaccatg agtgataaca ctgcggccaa cttacttctg acaacgatcg gaggaccgaa
     2521 ggagctaacc gcttttttgc acaacatggg ggatcatgta actcgccttg atcgttggga
     2581 accggagctg aatgaagcca taccaaacga cgagcgtgac accacgatgc ctgtagcaat
     2641 ggcaacaacg ttgcgcaaac tattaactgg cgaactactt actctagctt cccggcaaca
     2701 attaatagac tggatggagg cggataaagt tgcaggacca cttctgcgct cggcccttcc
     2761 ggctggctgg tttattgctg ataaatctgg agccggtgag cgtgggtctc gcggtatcat
     2821 tgcagcactg gggccagatg gtaagccctc ccgtatcgta gttatctaca cgacggggag
     2881 tcaggcaact atggatgaac gaaatagaca gatcgctgag ataggtgcct cactgattaa
     2941 gcattggtaa ctgtcagacc aagtttactc atatatactt tagattgatt taaaacttca
     3001 tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc
     3061 ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc
     3121 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
     3181 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
     3241 cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt
     3301 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
     3361 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
     3421 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
     3481 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
     3541 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
     3601 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
     3661 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
     3721 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgg ctcgac
//
