LOCUS       sequence-163081-       10016 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Pmex-5:GFP::H2B::PEST::set-2 3'UTR construct used for 
            microinjection.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10016)
  AUTHORS   Kaymak E, Farley BM, Hay SA, Li C, Ho S, Hartman DJ, Ryder SP
  TITLE     Efficient generation of transgenic reporter strains and analysis of 
            expression patterns in Caenorhabditis elegans using Library MosSCI.
  JOURNAL   Dev Dyn. 2016 Jun 13. doi: 10.1002/dvdy.24426.
  PUBMED    27294288
REFERENCE   2  (bases 1 to 10016)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Dev Dyn. 
            2016 Jun 13. doi: 10.1002/dvdy.24426."
COMMENT     Sequence Label: pCFJ150-set2
FEATURES             Location/Qualifiers
     source          1..10016
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(146..734)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(226..245)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(905..1696,1697..1765))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGSDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     1528..1547
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(1766..1870)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     1938..1956
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(1994..2016)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     3842..3859
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     3843..3859
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    3867..3887
                     /gene="mutant version of attB"
                     /label=attB3
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     protein_bind    4472..4496
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     primer_bind     complement(4957..4977)
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
     primer_bind     5725..5753
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
     protein_bind    complement(5915..5939)
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(6429..6449)
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     CDS             8515..8526
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
ORIGIN
        1 tgtgaaattg ttatccgctg gtatcagctc actcaaaggc ggtaatacgg ttatccacag
       61 aatcagggga taacgcagga aagaacatgt gagcaaaagg ccagcaaaag accaggaacc
      121 gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca
      181 aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt
      241 ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc
      301 tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc
      361 tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc
      421 ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact
      481 tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg
      541 ctacagagtt cttgaagtgg tggcctaact acggctacac tagaagaaca gtatttggta
      601 tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca
      661 aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa
      721 aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg
      781 aaaactcacg ttaagggatt ttggtcatga gattatcaaa aaggatcttc acctagatcc
      841 ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa acttggtctg
      901 acagttacca atgcttaatc agtgaggcac ctatctcagc gatctgtcta tttcgttcat
      961 ccatagttgc ctgactcccc gtcgtgtaga taactacgat acgggagggc ttaccatctg
     1021 accccagtgc tgcaatgata ccgcgagacc cacgctcacc ggctccagat ttatcagcaa
     1081 taaaccagcc agccggaagg gccgagcgca gaagtggtcc tgcaacttta tccgcctcca
     1141 tccagtctat taattgttgc cgggaagcta gagtaagtag ttcgccagtt aatagtttgc
     1201 gcaacgttgt tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt
     1261 cattcagctc cggttcccaa cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa
     1321 aagcggttag ctccttcggt cctccgatcg ttgtcagaag taagttggcc gcagtgttat
     1381 cactcatggt tatggcagca ctgcataatt ctcttactgt catgccatcc gtaagatgct
     1441 tttctgtgac tggtgagtac tcaaccaagt cattctgaga atagtgtatg cggcgaccga
     1501 gttgctcttg cccggcgtca atacgggata ataccgcgcc acatagcaga actttaaaag
     1561 tgctcatcat tggaaaacgt tcttcggggc gaaaactctc aaggatctta ccgctgttga
     1621 gatccagttc gatgtaaccc actcgtgcac ccaactgatc ttcagcatct tttactttca
     1681 ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg
     1741 cgacacggaa atgttgaata ctcatactct tcctttttca atattattga agcatttatc
     1801 agggttattg tctcatgagc ggatacatat ttgaatgtat ttagaaaaat aaacaaatag
     1861 gggttccgcg cacatttccc cgaaaagtgc cacctgacgt ctaagaaacc attattatca
     1921 tgacattaac ctataaaaat aggcgtatca cgaggccctt tcgtctcgcg cgtttcggtg
     1981 atgacggtga aaacctctga cacatgcagc tcccggagac ggtcacagct tgtctgtaag
     2041 cggatgccgg gagcagacaa gcccgtcagg gcgcgtcagc gggtgttggc gggtgtcggg
     2101 gctggcttaa ctatgcggca tcagagcaga ttgcactgag agtgcaccat atgcggtgtg
     2161 aaataccgca cagatgcgta aggagaaaat accgcatcag gcgccattcg ccattcaggc
     2221 tgcgcaactg ttgggaaggg cgatcggtgc gggcctcttc gctattacgc cagctggcga
     2281 aagggggatg tgctgcaagg cgattaagtt gggtaacgcc agggttttcc ccagtcacga
     2341 cgtcacagca cgtgtgtttt tgaacacggc gatatgtatc tgtagatcat ccccaatttt
     2401 caacaatgaa tctaaatact gaactttttg tgtaatcttt agtgcagaaa aatgaccaaa
     2461 atcagcgatt ttccgattat acccaaaata tctcagctca atgagcacct ataaaaatgt
     2521 aaaaataaag aaagtagtcg aaccatattt tgtaaaggct aaaaaactga ataaattttt
     2581 atccatccag cgcggcgtta caatccgtgg cccatgtcac gcacggcttt caaagaaaaa
     2641 aaaacagtct gtttgcgcga catcgatctg gcaaaaatcc taaaaacgac tatgcgtggc
     2701 tcactttata ttaatatgaa aaatacaaac ttggtggtgc aggtgctgca gaggccatcg
     2761 atgaagaagt ggaggccttg ttgtcgatct acaaaattgt atggttgttt gagattttcc
     2821 ttgaaacgct ctcaatcttg gatatgatat aaattctgga taagatatga tggatatgat
     2881 ctatgtttga tagagtgcat ttgaaaggat aacggccctg cagacatttt tgaaaaaata
     2941 acaaacattt aattttctca ctcgtttagg ctattcccct atttttgata ttccttcgca
     3001 catatgaaaa ctactttttt tcgaaactgt taactccaga atttataaat ctatagccct
     3061 tacttgatta tttattatca tggttactca cctcatgtct ctttctcttt tcaagaagtc
     3121 ttcgttcata tctgttgtct ctttcctcct tttgaatctc tgctttgctc ttcgccattg
     3181 tttcctgaaa ataatgtaac ttgaattgtg taatatactt ttttaatttg aatttggctt
     3241 gtaacgcgga atcactacgt gcgggatcat ttcttactag aaaacccaga aaatgccata
     3301 tttcacttat ctcggggtca tttctaatta gaaaagctac aaaaaccatt tctaattacg
     3361 gtgactttag aactggtcga aatagctaat tagaaatggg tttttgctaa ttagaggtga
     3421 ctctatgtta tctgagtaag cctcgtttgt atgtaaactc acagtactaa taaatgcaag
     3481 acacccgggt ttgtctagat atgaaataat tgaaatatca attctgacag acaataatgg
     3541 taatcttgat aaggagttcc acgcccagga gaacacgtta gttttcttgt ttttgattgc
     3601 gtgcgttatt ttggagaaaa actcgatttt ttacaaaata attttttgaa aggaacactg
     3661 ttcaataagt tttgtctttt ttctcagttg tgatacggtt ttttattctt ttttgtagtt
     3721 atacagaaga ccgttacgaa acagactgat atcgaaacaa acactgatct tacttgcact
     3781 tataatacga ctcactagtg ggcagatctt cgaatgcatc gcgcgcaccg tacgtctcga
     3841 gtgtaaaacg acggccagtg aattatcaac tatgtataat aaagttgacg aaaaccatga
     3901 cttttaaaat tatttcattt aaagaacata ataaaacact ttttaaaaca taaaactatt
     3961 cagactagct gaaattaact ttaaacacga atgaataaag acttgaatgg gaggttgttt
     4021 tggaaagacc aattgttcag agttcaaggg aaaatgaatg atttcagaga tgttcaagta
     4081 tagagagata aacaagagac tgtagaggaa atcctccaag aaaacaattc gtaaacctat
     4141 agagaaacaa gagatttgat aaaatgtaga agtatctagt tgaaaagggg agcttgtatc
     4201 gtataacata cactcaaaca attgttcaac aataacgaat tccactaata atacaaggat
     4261 ttctcagctc accataagga gaatgaataa caatgataca gttttcaata tttaaaaaaa
     4321 aagtaggaac cggcctattc ttatcagata gcaatttcca acttcgaacc agcgaattgg
     4381 gaattggata ggaaaattta aataaataaa aaatattaaa aaactagaag agatcgaatg
     4441 gaatataaat tttaaaaact agacgaatca taccactttg tacaagaaag ctgggtttac
     4501 ttgctggaag tgtacttggt gacggccttg gttccctcag acacggcgtg cttggcaagt
     4561 tctcctggga gaatcaaacg gacagcggtt tgaatttcgc gggatgagat cgttgagcgt
     4621 ttgttgtaat gagcaagacg ggaagcttcc gaagcgatgc gttcgaatac atcgttgacg
     4681 aaggagttca tgatagacat ggccttggag gagactccgg tgtctgggtg aacttgcttg
     4741 agaacacggt agatgtagac ggagtacgat tccttgcggg catgacgtct cttctttccg
     4801 tcctttggct tggcaacgac ggtcttggcg gccttcttgg ctcccttggc agatggcttt
     4861 ggtggactag ttctggagcg gccgccaccg gtggagctcc accgatcccc cgggcatttg
     4921 tatagttcat ccatgccatg tgtaatccca gcagctgtta caaactcaag aaggaccatg
     4981 tggtctctct tttcgttggg atctttcgaa agggcagatt gtgtggacag gtaatggttg
     5041 tctggtaaaa ggacagggcc atcgccaatt ggagtatttt gttgataatg gtctgctagt
     5101 tgaacgcttc catcttcaat gttgtgtcta attttgaagt tctgaaaatt taaatcagat
     5161 tagttagtta gtaaaatcat gtttaaactt acaactttga ttccattctt ttgtttgtct
     5221 gccatgatgt atacattgtg tgagttatag ttgtattcca atttgtgtcc aagaatgttt
     5281 ccatcttctt taaaatcaat accttttaac tcgattctat taacaagggt atcaccttca
     5341 aacttgactt cagcacctga aaatttaaat atgtatggtt agttagtacc gaactgttta
     5401 aacttacgtg tcttgtagtt cccgtcatct ttgaaaaata tagttctttc ctgtacataa
     5461 ccttcgggca tggcactctt gaaaaagtca tgccgtttca tatgatctgg gtatctcgag
     5521 aagcattgaa caccataaca gaaagtagtg acaagtgttg gctgaaaatt taaataatca
     5581 gggttagtta gtatatatat gtttaaactt acccatggaa caggtagttt tccagtagtg
     5641 caaataaatt taagggtaag ttttccgtat gttgcatcac cttcaccctc tccactgaca
     5701 gaaaatttgt gcccattaac atcaccatct aattcaacaa gaattgggac aactccagtg
     5761 aaaagttctt ctcctctaga cacattgatc ctagcagaag cacaggctgc agggtgacgg
     5821 tccatcccgc tctcctgggc acaagacatg ggcagcgtgc catcatcctg cgccgccacc
     5881 gccggcggga agccatggct aagtctagac atggagcctg cttttttgta caaacttgtc
     5941 attctctgtc tgaaacattc aattgattat cgataaaatt aattaagaga gaatacaatg
     6001 agaaaacgga aaatcgagca ggattttggg gaaagtcggg gaatttttgc tatttaagct
     6061 ttctttaaca aagaaactgc atttttccat gagattacca tgaaatttgc gttaaatggg
     6121 tgaatttata acatttacgg ctagaaatta attttaaaaa agagaataaa cgaaaaatta
     6181 ttcgagaaaa cgtctttgaa gcagaaatga aatgaaaaaa caaccgccag cgctgacaaa
     6241 aatgatcaaa caaacggagt acggtatacg cggtaagacc caaatgaatt ttaattcggt
     6301 gcgcctttaa aaaatgtatt tttttaattt ctttatccgt gggcccaaat gtattttttt
     6361 cttgtggttt gatgtaaaaa ttgggttata ttatttgttt tatggtttaa ttttttaaaa
     6421 actgatatca acttttctat acaaagttga tagcttggcg taatcatggt catagctgtt
     6481 cctgcctagg tgagctctgg taccctctag tcaaggcctt aagtgagtcg tattacgtag
     6541 cccgggccta gttctagaca ttctctaatg aaaaaatctt tcagttgaaa ttgaaaatga
     6601 gttaaagttg gagtttttat tgaaaacaga tttccgtgtg attagtgttt ttagcgagtg
     6661 tgacaggaca gcgaaaaaat atagaaacaa ggggggaact gaaaagctta ggaatgcatt
     6721 gaacatgaga aggggaaggg gaaggaacaa actagacagg aattattgga atttaatcac
     6781 atttggagtt ttttttctat tcgacagaat aattatccag aacatttttg tattaaatat
     6841 ttatgcatca tatgagtagt cggctttgtt gtgcatgacg agtttgttat cgacgaaata
     6901 gaagctgtca gaacgagtct cgtttggatt gttgatcatg tcgtccactg aaaaagagat
     6961 tagtcttttg aattgtactt tttagaataa tgactcactg agttgttgag agagttgagg
     7021 gaactcatag atatgttcac agttgtttcg tgaattcgga atacagaatc cgaattcaaa
     7081 gtcaaaacac ttcagaaggc gatctttgaa gaagtgacgt tcgatcattc ggaaatgatg
     7141 gattgggatg tctcctactt tgaattccac agttgctccg accgttttga gtttcaaaaa
     7201 gtttggagca aatctataac gcacgtatct tgccgactcc tgtggcgact catcattctc
     7261 ctgatcgttc tccggtttgg cgatctcgaa aagcacttgc tcagtgtcca gatcacggat
     7321 ttggaacttg gtgaactcga tgttatagat gttcgcagat ggggagcata agaatcctaa
     7381 atttatgttt taaactgaaa tccaaaggga gcaagatacc ttgagtgatt cccggaagtg
     7441 ctaaaacgtc gttcggagtg atttgagctt tcttcgcaag ctccgattcc gttgtgattc
     7501 cttgttcggt gcttggtggt ggccgtggca tctggaaata tggaaaagtt caacaaaaag
     7561 aaaagagaaa agaatgaaat cggatatcaa gagttagttg agcggtttct ctagttttct
     7621 gagtctcacc tgcgacggga aggtcgccga gccgggtgga atcgatcgtt gttgctcggc
     7681 tttcatatcg gtttggttgg aagcggctga aaacggaaag aagtggaaga aggaaaagag
     7741 tgtggtgtga caggaaaatg gtaattagag ggtgccaaat aaccagctat attttgtttt
     7801 tttttgaaaa catttttaaa aagaaaaata cgataatgat atcagatgga tttccggaaa
     7861 actggtatga aaaatttcaa cctttttgag tacatgtaat caaaatacac tttgtaaatt
     7921 atcattttta ttgaaactcc accatttttc tatttataac gctaataatt tgaaaaagaa
     7981 acctattgcg aaccgcgggg tgaatcccaa aaacgaatgc gttttggtgg agtgattgat
     8041 tcgaatcgaa gaagaaaaag aagaagacgt ggaatagaga gctcactctt aaccgagcag
     8101 cacacaccga cagaaaaaaa aatgaaatga atgagggtct tcttcttctt cttcttcgaa
     8161 tgattgacag aaatgggaaa aagaggaaga ttgagaaggg aaaaaggaag gagaaaagaa
     8221 gcagaagaag acgtcagaga ggagaggaac gagcggaaaa gcagcgggcg caagtcatag
     8281 aagtagcaga gctggggaga agaagacact atccaagaaa ggaatgacga gagagtatgc
     8341 aaaggggtat agggtgcaga cagaatagga acagaataac agatgatgag ccaagaagag
     8401 ttgaaaaggg cgatgaattt gtcatgtaac ttaatttggg tcaatttgag catgatgaat
     8461 tgaaatcatc ccttgttggg agttaataac cggtttgtta tcagaaaccc tgtaatagaa
     8521 gggcgcccta actttgagcc aattcatccc ggtttctgtc aaatatatca aaaagtggtc
     8581 aactgacaaa ttgtttttga tattataata aacattttat ccgttaacaa ttttcgaata
     8641 ctttttacaa ggacttggat aaattggctc aaagacgaag tgagtttgct accatcatgg
     8701 aatcaggagg acatttcgac aatgtcatat aatgtttgaa aaatgatttt aacaagggtc
     8761 tcattcgaga taagcaatgc tctgagcaag tggctcgtcg tcttcaagaa tctcttgttg
     8821 atattcattt ggatcctgtt ttgcacgaag catgtgccat ggatattcag agtttttgca
     8881 aaaaagttcc gccaggtcaa acaatctttt taatttttat atgatacttc gggtgtattt
     8941 ttcttctttt tcctgttcat tttcgctttt ttctcacttt tttgtaacaa aatctctcac
     9001 ttttctgtaa gtcttaacaa aagttcaatt ggctcgatca agtaaaattt cggccgtatg
     9061 aacgttttca aaggttttca ttttttttta agttcattca caatatcatg aatctctcgt
     9121 cctttggttt ccggtaagta gcgaaataat ataagcatac ttataaatga aggaattgaa
     9181 tatagaataa ggaaagcata actaccaata acggaatata gaggtaatac tgtaaatgta
     9241 ctgataacca ccattacagt attcattgaa tatgccacag attgagcaat actacgatgt
     9301 ttttgaggta ccagttcaga ggatataaac cacgaaattg gaccaactcc agttctgaaa
     9361 tttcattatt cgattaaatc aaaatatatt tgaaaaacct cacccataag tataaccgta
     9421 gatgatcaat gcaactaggc atccgtattt catcgggtca atcagaatac gtatttttgc
     9481 gaagaatacg aatgcaatta gagctaaatt attgagaaat gtgaagagaa ggagaagctt
     9541 gcgtcgacca actctgtaaa cttattcata caattttgtc ttcaaatttt gaacgtttaa
     9601 aactatctta ttcaatgagt tagcgggttt ttatagtgag ttagttcacg attatctaat
     9661 aaaactatgt ccaaacatcc taactgcgac cgtattctca ctaactcgtc ttaaaaacgt
     9721 tgagattatt ttaaaccctt cttcagtaca actacagtac tgatgcacaa atacaataat
     9781 aataatacat tcagacatac tctgaacaat gtaacttgtt tttgttatac tgtggtaaca
     9841 tttattgaaa tgaaatataa ccttactttt caatcacaat tccaccagta attgttccag
     9901 aaacataagc taaacccatt gcagttgtac tccattcagc aatttctgac tcaacatttg
     9961 cattttctag aaagaaagta gaggataaaa gaagagccca taatccgaca gggccc
//
