LOCUS       sequence-163086-         829 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  sgRNA targeting murine CD45.2 region 3. Co-expresses 
            SpCas9-2A-EGFP..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 829)
  AUTHORS   Kornete M, Marone R, Jeker LT
  TITLE     Highly Efficient and Versatile Plasmid-Based Gene Editing in Primary
            T Cells.
  JOURNAL   J Immunol. 2018 Feb 14. pii: jimmunol.1701121. doi: 
            10.4049/jimmunol.1701121.
  PUBMED    29445007
REFERENCE   2  (bases 1 to 829)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Immunol. 
            2018 Feb 14. pii: jimmunol.1701121. doi: 10.4049/jimmunol.1701121."
COMMENT     Sequence Label: p206_LTJ_sgRNACD45.2_R3
FEATURES             Location/Qualifiers
     source          1..829
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(21..74)
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             complement(90..137)
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
ORIGIN
        1 agctcctcgc ccttgctcac tgggccagga ttctcctcga cgtcaccgca tgttagcaga
       61 cttcctctgc cctctccact gccgaattcc tttttctttt ttgcctggcc ggcctttttc
      121 gtggccgccg gccttttgtc gcctcccagc tgagacaggt cgatccgtgt ctcgtacagg
      181 ccggtgatgc tctggtggat cagggtggcg tccagcacct ctttggtgct ggtgtacctc
      241 ttccggtcga tggtggtgtc aaagtacttg aaggcggcag gggctcccag attggtcagg
      301 gtaaacaggt ggatgatatt ctcggcctgc tctctgatgg gcttatcccg gtgcttgttg
      361 taggcggaca gcactttgtc cagattagcg tcggccagga tcactctctt ggagaactcg
      421 ctgatctgct cgatgatctc gtccaggtag tgcttgtgct gttccacaaa cagctgtttc
      481 tgctcattat cctcggggga gcccttcagc ttctcatagt ggctggccag gtacaggaag
      541 ttcacatatt tggagggcag ggccagttcg tttcccttct gcagttcgcc ggcagaggcc
      601 agcattctct tccggccgtt ttccagctcg aacagggagt acttaggcag cttgatgatc
      661 aggtcctttt tcacttcttt gtagcccttg gcttccagaa agtcgatggg attcttctcg
      721 aagctgcttc tttccatgat ggtgatcccc agcagctctt tcacactctt cagtttcttg
      781 gacttgccct tttccacttt ggccaccacc agcacagaat aggccacgg
//
