LOCUS       sequence-163093-         917 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  2x35S promoting Glycing Max codon optimized Cas9 with hygromycin 
            plant selection.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 917)
  AUTHORS   Michno JM, Wang X, Liu J, Curtin SJ, Kono TJ, Stupar RM
  TITLE     CRISPR/Cas mutagenesis of soybean and Medicago truncatula using a 
            new web-tool and a modified Cas9 enzyme.
  JOURNAL   GM Crops Food. 2015;6(4):243-52. doi: 10.1080/21645698.2015.1106063.
  PUBMED    26479970
REFERENCE   2  (bases 1 to 917)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "GM Crops 
            Food."; date: "2015"; volume: "6(4):243-52. doi"; pages: " 
            10.1080/21645698.2015.1106063"
COMMENT     Sequence Label: Cas9 MDC32
FEATURES             Location/Qualifiers
     source          1..917
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(165..841)
                     /label=CaMV 35S promoter (enhanced)
                     /note="cauliflower mosaic virus 35S promoter with a 
                     duplicated enhancer region"
     primer_bind     complement(208..228)
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
ORIGIN
        1 tcttcgccct ccgagagctg catcaggtcg gagacgctgt cgaacttttc gatcagaaac
       61 ttctcgacag acgtcgcggt gagttcaggc tttttcatat ctcattgccc cccggatctg
      121 cgaaagctcg agagagatag atttgtagag agagactggt gatttcagcg tgtcctctcc
      181 aaatgaaatg aacttcctta tatagaggaa gggtcttgcg aaggatagtg ggattgtgcg
      241 tcatccctta cgtcagtgga gatatcacat caatccactt gctttgaaga cgtggttgga
      301 acgtcttctt tttccacgat gctcctcgtg ggtgggggtc catctttggg accactgtcg
      361 gcagaggcat cttgaacgat agcctttcct ttatcgcaat gatggcattt gtaggtgcca
      421 ccttcctttt ctactgtcct tttgatgaag tgacagatag ctgggcaatg gaatccgagg
      481 aggtttcggg atattaccct ttgttgaaaa gtctcaatag ccctttggtc ttctgagact
      541 gtatctttga tattcttgga gtagacgaga gtgtcgtgct ccaccatgtt cacatcaatc
      601 cacttgcttt gaagacgtgg ttggaacgtc ttctttttcc acgatgctcc tcgtgggtgg
      661 gggtccatct ttgggaccac tgtcggcaga ggcatcttga acgatagcct ttcctttatc
      721 gcaatgatgg catttgtagg tgccaccttc cttttctact gtccttttga tgaagtgaca
      781 gatagctggg caatggaatc cgaggaggtt tcccgatatt accctttgtt gaaaagtctc
      841 aatagccctt tggtcttctg agactgtatc tttgatattc ttggagtaga cgagagtgtc
      901 gtgctccacc atgttgg
//
