LOCUS       sequence-163103-         986 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Modified from pCFD4, with the vermillion marker and the attB site 
            removed.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 986)
  AUTHORS   Ge DT, Tipping C, Brodsky MH, Zamore PD
  TITLE     Rapid Screening for CRISPR-Directed Editing of the Drosophila Genome
            Using white Co-conversion.
  JOURNAL   G3 (Bethesda). 2016 Aug 26. pii: g3.116.032557. doi: 
            10.1534/g3.116.032557.
  PUBMED    27543296
REFERENCE   2  (bases 1 to 986)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "G3 
            (Bethesda). 2016 Aug 26. pii: g3.116.032557. doi: 
            10.1534/g3.116.032557."
COMMENT     Sequence Label: pCFD4d
FEATURES             Location/Qualifiers
     source          1..986
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        34..830
                     /label=dU6-1 promoter
                     /note="RNA polymerase III promoter for Drosophila U6-1 
                     snRNA (Port et al., 2014)"
     misc_RNA        849..924
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
ORIGIN
        1 tagggcgaat tgggtacaag ctgatctgaa ttcattttca acgtcctcga tagtatagtg
       61 gttagtatcc ccgcctgtca cgcgggagac cggggttcaa ttccccgtcg gggagaatct
      121 gtgattcttt ttttttttct tttactttgt tatataaaca atttttgttt taattgaatc
      181 taatttgcca ttgcttttag gaatctcagg catccagcaa gcgtttgtcc gccgaatcgc
      241 ccatcagtga agaagatcct gtggcggcta cgaaaatctc cccggccatg tcggcctcca
      301 cctccagcga aaaacccatc agcgagctgg ccacctctgt gctgacccac cgctttccag
      361 actccacctc ctcacccggc gaacatggcc ttggacgaat gcagttgtcg atccgctaca
      421 gcgcccagcg tcaaaaacta gacgtgacca tacacaaaat ccagaagata ccacttcgcg
      481 atcccagcaa tatccccgat ccgtatgtta agctgtatct gttgcctgga cgcaccaagg
      541 agtcgaaacg caagacgagc gtgatcaagg acaactgcaa ccccgtctac gatgcatcct
      601 ttgagtacct gatttccatt gccgaactca ggcagacgga actggaggtg acggtgtgca
      661 cccaaaaggg attcctatcc ggcggtagtc ccatcattgg catggtaggt acccgaaagc
      721 aaccccttag ttacagacac agcgcgtacg tccttcgcat ccttatgatt cccaagtaca
      781 tattctgcaa gagtacagta tatataggaa agatatccgg gtgaacttcg gggtcttcga
      841 gaagacctgt tttagagcta gaaatagcaa gttaaaataa ggctagtccg ttatcaactt
      901 gaaaaagtgg caccgagtcg gtgctttttt gctcacctgt gattgctcct actcaaatac
      961 aaaaacatca aattttctgt caataa
//
