LOCUS       sequence-163110-         894 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  pCFD4d expresses white sgRNA-1 from a U6:3 promoter and the same 
            white sgRNA-1 from a U6:1 promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 894)
  AUTHORS   Ge DT, Tipping C, Brodsky MH, Zamore PD
  TITLE     Rapid Screening for CRISPR-Directed Editing of the Drosophila Genome
            Using white Co-conversion.
  JOURNAL   G3 (Bethesda). 2016 Aug 26. pii: g3.116.032557. doi: 
            10.1534/g3.116.032557.
  PUBMED    27543296
REFERENCE   2  (bases 1 to 894)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "G3 
            (Bethesda). 2016 Aug 26. pii: g3.116.032557. doi: 
            10.1534/g3.116.032557."
COMMENT     Sequence Label: pCFD4d-U6-1:white1-U6-3:white1
FEATURES             Location/Qualifiers
     source          1..894
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     3..23
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        5..23
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     misc_RNA        complement(334..409)
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     promoter        complement(430..850)
                     /label=dU6-3 promoter
                     /note="RNA polymerase III promoter for Drosophila U6-3 
                     snRNA (Port et al., 2014)"
ORIGIN
        1 gcgcaattaa ccctcactaa agggaacaaa agctggagct cctgcaggtt gttggttggc
       61 acaccacaaa tatactgttg ccgagcacaa ttgtctagaa tgcatacgca ttaagcgaac
      121 attaaaaaga tgtgaaaaca taactattat gtctaaataa acacacgtca gatgtatgta
      181 cgtcaacgga aaaccattgt ctatatatta caattactaa atacatacca aattgaatac
      241 atattgatga aaaataataa atactggcga aagcaaaaaa acgaaacatt tttattttat
      301 tgaacaactc tcaggctcca ggtaggcaaa aaagcaccga ctcggtgcca ctttttcaag
      361 ttgataacgg actagcctta ttttaacttg ctatttctag ctctaaaacc caaagagcag
      421 gaatggtatc gacgttaaat tgaaaatagg tctatatata cgaactgagt ctggaaaaag
      481 aagttgagaa ttataaaaag tagtgagcac tggcgccctc tctgcttggc gagctaacct
      541 tttcgcctct tggctgagta ggtggcgttt cattctactc tgtaaaatta atgtagaatt
      601 gaaacactat ggtcaaaaaa tacttagggg cataagttat aaaacgtatg aaatgaattt
      661 ttatcaacct gggctattca aaaattttcg aattatttta tgtatttttt ttaatcgttt
      721 ttcattatag gttaaaatac actttaaaag gaattctttc ctgtaaaata aatataaata
      781 aatatgcttt attgacagaa aatttgatgt ttttgtattt gagtaggagc aatcacaggt
      841 gagcaaaaaa gcaccgactc ggtgccactt tttcaagttg ataacggact agcc
//
