LOCUS       sequence-163114-        3975 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway Donor vector containing MAP2K1 , part of the Target 
            Accelerator Plasmid Collection..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3975)
  AUTHORS   Kim E, Ilic N, Shrestha Y, Zou L, Kamburov A, Zhu C, Yang X, Lubonja
            R, Tran N, Nguyen C, Lawrence MS, Piccioni F, Bagul M, Doench JG, 
            Chouinard CR, Wu X, Hogstrom L, Natoli T, Tamayo P, Horn H, Corsello
            SM, Lage K, Root DE, Subramanian A, Golub TR, Getz G, Boehm JS, Hahn
            WC
  TITLE     Systematic Functional Interrogation of Rare Cancer Variants 
            Identifies Oncogenic Alleles.
  JOURNAL   Cancer Discov. 2016 Jul;6(7):714-26. doi: 
            10.1158/2159-8290.CD-16-0160. Epub 2016 May 4.
  PUBMED    27147599
REFERENCE   2  (bases 1 to 3975)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1158/2159-8290.CD-16-0160"; journalName: "Cancer Discov"; date: 
            "2016-07"; volume: "6"; issue: "7"; pages: "714-26"
COMMENT     Sequence Label: pDONR223_MAP2K1_WT
FEATURES             Location/Qualifiers
     source          1..3975
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     43..65
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     57..74
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     58..74
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    90..189
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(1375..1474)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(1491..1510)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(1492..1510)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(1515..1531)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1515..1531)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             1962..2753
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      2846..3434
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3335..3354
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3588..3605
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(3764..3791)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(3883..3969)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
ORIGIN
        1 ggcagttccc tactctcgcg ttaacgctag catggatgtt ttcccagtca cgacgttgta
       61 aaacgacggc cagtcttaag ctcgggcccc aaataatgat tttattttga ctgatagtga
      121 cctgttcgtt gcaacaaatt gatgagcaat gcttttttat aatgccaact ttgtacaaaa
      181 aagttggcac catgcccaag aagaagccga cgcccatcca gctgaacccg gcccccgacg
      241 gctctgcagt taacgggacc agctctgcgg agaccaactt ggaggccttg cagaagaagc
      301 tggaggagct agagcttgat gagcagcagc gaaagcgcct tgaggccttt cttacccaga
      361 agcagaaggt gggagaactg aaggatgacg actttgagaa gatcagtgag ctgggggctg
      421 gcaatggcgg tgtggtgttc aaggtctccc acaagccttc tggcctggtc atggccagaa
      481 agctaattca tctggagatc aaacccgcaa tccggaacca gatcataagg gagctgcagg
      541 ttctgcatga gtgcaactct ccgtacatcg tgggcttcta tggtgcgttc tacagcgatg
      601 gcgagatcag tatctgcatg gagcacatgg atggaggttc tctggatcaa gtcctgaaga
      661 aagctggaag aattcctgaa caaattttag gaaaagttag cattgctgta ataaaaggcc
      721 tgacatatct gagggagaag cacaagatca tgcacagaga tgtcaagccc tccaacatcc
      781 tagtcaactc ccgtggggag atcaagctct gtgactttgg ggtcagcggg cagctcatcg
      841 actccatggc caactccttc gtgggcacaa ggtcctacat gtcgccagaa agactccagg
      901 ggactcatta ctctgtgcag tcagacatct ggagcatggg actgtctctg gtagagatgg
      961 cggttgggag gtatcccatc cctcctccag atgccaagga gctggagctg atgtttgggt
     1021 gccaggtgga aggagatgcg gctgagaccc cacccaggcc aaggaccccc gggaggcccc
     1081 ttagctcata cggaatggac agccgacctc ccatggcaaa ttttgagttg ttggattaca
     1141 tagtcaacga gcctcctcca aaactgccca gtggagtgtt cagtctggaa tttcaagatt
     1201 ttgtgaataa atgcttaata aaaaaccccg cagagagagc agatttgaag caactcatgg
     1261 ttcatgcttt tatcaagaga tctgatgctg aggaagtgga ttttgcaggt tggctctgct
     1321 ccaccatcgg ccttaaccag cccagcacac caacccatgc tgctggcgtc taattgccaa
     1381 ctttcttgta caaagttggc attataagaa agcattgctt atcaatttgt tgcaacgaac
     1441 aggtcactat cagtcaaaat aaaatcatta tttgccatcc agctgatatc ccctatagtg
     1501 agtcgtatta catggtcata gctgtttcct ggcagctctg gcccgtgtct caaaatctct
     1561 gatgttacat tgcacaagat aaaaatatat catcatgcct cctctagacc agccaggaca
     1621 gaaatgcctc gacttcgctg ctacccaagg ttgccgggtg acgcacaccg tggaaacgga
     1681 tgaaggcacg aacccagtgg acataagcct gttcggttcg taagctgtaa tgcaagtagc
     1741 gtatgcgctc acgcaactgg tccagaacct tgaccgaacg cagcggtggt aacggcgcag
     1801 tggcggtttt catggcttgt tatgactgtt tttttggggt acagtctatg cctcgggcat
     1861 ccaagcagca agcgcgttac gccgtgggtc gatgtttgat gttatggagc agcaacgatg
     1921 ttacgcagca gggcagtcgc cctaaaacaa agttaaacat tatgagggaa gcggtgatcg
     1981 ccgaagtatc gactcaacta tcagaggtag ttggcgtcat cgagcgccat ctcgaaccga
     2041 cgttgctggc cgtacatttg tacggctccg cagtggatgg cggcctgaag ccacacagtg
     2101 atattgattt gctggttacg gtgaccgtaa ggcttgatga aacaacgcgg cgagctttga
     2161 tcaacgacct tttggaaact tcggcttccc ctggagagag cgagattctc cgcgctgtag
     2221 aagtcaccat tgttgtgcac gacgacatca ttccgtggcg ttatccagct aagcgcgaac
     2281 tgcaatttgg agaatggcag cgcaatgaca ttcttgcagg tatcttcgag ccagccacga
     2341 tcgacattga tctggctatc ttgctgacaa aagcaagaga acatagcgtt gccttggtag
     2401 gtccagcggc ggaggaactc tttgatccgg ttcctgaaca ggatctattt gaggcgctaa
     2461 atgaaacctt aacgctatgg aactcgccgc ccgactgggc tggcgatgag cgaaatgtag
     2521 tgcttacgtt gtcccgcatt tggtacagcg cagtaaccgg caaaatcgcg ccgaaggatg
     2581 tcgctgccga ctgggcaatg gagcgcctgc cggcccagta tcagcccgtc atacttgaag
     2641 ctagacaggc ttatcttgga caagaagaag atcgcttggc ctcgcgcgca gatcagttgg
     2701 aagaatttgt ccactacgtg aaaggcgaga tcaccaaggt agtcggcaaa taaccctcga
     2761 gccacccatg accaaaatcc cttaacgtga gttacgcgtc gttccactga gcgtcagacc
     2821 ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct
     2881 tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa
     2941 ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact gttcttctag
     3001 tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc
     3061 tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg
     3121 actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca
     3181 cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat
     3241 gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg
     3301 tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc
     3361 ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc
     3421 ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc
     3481 cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg
     3541 cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga
     3601 gcgaggaagc ggaagagcgc ccaatacgca aaccgcctct ccccgcgcgt tggccgattc
     3661 attaatgcag ctggcacgac aggtttcccg actggaaagc gggcagtgag cgcaacgcaa
     3721 ttaatacgcg taccgctagc caggaagagt ttgtagaaac gcaaaaaggc catccgtcag
     3781 gatggccttc tgcttagttt gatgcctggc agtttatggc gggcgtcctg cccgccaccc
     3841 tccgggccgt tgcttcacaa cgttcaaatc cgctcccggc ggatttgtcc tactcaggag
     3901 agcgttcacc gacaaacaac agataaaacg aaaggcccag tcttccgact gagcctttcg
     3961 ttttatttga tgcct
//
