LOCUS       sequence-163133-        4650 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway Donor vector containing MAP3K2 , part of the Target 
            Accelerator Plasmid Collection..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4650)
  AUTHORS   Kim E, Ilic N, Shrestha Y, Zou L, Kamburov A, Zhu C, Yang X, Lubonja
            R, Tran N, Nguyen C, Lawrence MS, Piccioni F, Bagul M, Doench JG, 
            Chouinard CR, Wu X, Hogstrom L, Natoli T, Tamayo P, Horn H, Corsello
            SM, Lage K, Root DE, Subramanian A, Golub TR, Getz G, Boehm JS, Hahn
            WC
  TITLE     Systematic Functional Interrogation of Rare Cancer Variants 
            Identifies Oncogenic Alleles.
  JOURNAL   Cancer Discov. 2016 Jul;6(7):714-26. doi: 
            10.1158/2159-8290.CD-16-0160. Epub 2016 May 4.
  PUBMED    27147599
REFERENCE   2  (bases 1 to 4650)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1158/2159-8290.CD-16-0160"; journalName: "Cancer Discov"; date: 
            "2016-07"; volume: "6"; issue: "7"; pages: "714-26"
COMMENT     Sequence Label: pDONR223_MAP3K2_WT
FEATURES             Location/Qualifiers
     source          1..4650
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     43..65
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     57..74
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     58..74
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    90..189
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(2050..2149)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(2166..2185)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(2167..2185)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2190..2206)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2190..2206)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             2637..3428
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      3521..4109
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4010..4029
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     4263..4280
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(4439..4466)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(4558..4644)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
ORIGIN
        1 ggcagttccc tactctcgcg ttaacgctag catggatgtt ttcccagtca cgacgttgta
       61 aaacgacggc cagtcttaag ctcgggcccc aaataatgat tttattttga ctgatagtga
      121 cctgttcgtt gcaacaaatt gatgagcaat gcttttttat aatgccaact ttgtacaaaa
      181 aagttggcac catggatgat cagcaagctt tgaactcaat catgcaagat ttggctgtcc
      241 ttcataaggc cagtcgacca gcattatcct tgcaggaaac cagaaaagca aaatcttcat
      301 caccaaaaaa acagaatgat gtccgagtca aatttgaaca tagaggagaa aaaagaatcc
      361 ttcagttccc cagaccagtt aaactggaag atctgagatc taaagctaaa attgcctttg
      421 gacagtctat ggatctacat tataccaata acgagttggt aattccatta actactcaag
      481 atgacttgga caaagctgtg gaactgctgg atcgtagtat tcatatgaag agcctcaaga
      541 tattacttgt aataaatgga agtacacagg ctactaattt agaaccattg ccatcactag
      601 aagatttgga taatacagta tttggagcag agaggaaaaa acggctatct ataataggtc
      661 ctactagtag agatagaagt tctcctcccc caggttacat tccagatgaa ttacaccagg
      721 ttgcccggaa tgggtcattc actagtatca acagtgaagg agagttcatt ccagagagca
      781 tggaccaaat gctggatcca ttatctttaa gcagccctga aaattctggc tcaggaagtt
      841 gtccatcact tgatagtcct ttggatggag agagctatcc aaaatcacga atgcctaggg
      901 ctcagagcta cccagataat catcaggaat tttcagacta tgataaccct atctttgaga
      961 aatttggaaa aggaggaaca tatccaagaa ggtatcatgt ttcatatcat catcaagagt
     1021 ataatgatgg tcgtaaaact tttccaagag ctagaaggac ccaggggacc agcttacggt
     1081 ctcctgtgag tttcagtcct actgatcatt ccttaagcac tagtagtgga agcagtatct
     1141 ttaccccaga gtatgatgat agtcgaataa gaagaagggg aagtgacata gacaatccta
     1201 ctttgaccgt aatggacatc agcccaccca gccgttcacc tcgagctccg accaactgga
     1261 gattgggcaa actgcttggc caaggagcct ttggaagggt ctacctctgt tatgatgttg
     1321 atacaggaag agaattggct gttaagcaag ttcaatttga ccccgatagt cctgagacca
     1381 gcaaggaagt aaatgcactt gagtgtgaaa ttcagttgct gaaaaacttg ctacatgagc
     1441 gaattgttca gtattatggc tgtttgaggg atccccagga aaaaacactt tccatattta
     1501 tggaatatat gccagggggt tcaattaagg accaattaaa agcatatggc gctcttactg
     1561 agaatgtgac taggaaatac acccgtcaga ttctggaggg tgtccattat ttgcacagta
     1621 atatgattgt ccatagagat atcaaaggcg caaatatcct gcgagattca acaggcaacg
     1681 tcaaactagg agattttggg gccagcaaac ggcttcagac catctgtctc tcagggacag
     1741 gaatgaagtc tgtcacgggc acaccatact ggatgagccc tgaagtcatc agtggagaag
     1801 gctatggaag aaaagcagac atctggagtg ttgcatgtac tgtggtagaa atgctaactg
     1861 aaaagccgcc ttgggctgaa tttgaagcaa tggctgccat ctttaaaatc gccactcagc
     1921 caacaaaccc aaagctgcca cctcatgtct cagactatac tcgagatttc ctcaaacgga
     1981 tttttgtaga ggccaaactg agaccttcag ctgatgaact cttaaggcac atgtttgtgc
     2041 attatcactt gccaactttc ttgtacaaag ttggcattat aagaaagcat tgcttatcaa
     2101 tttgttgcaa cgaacaggtc actatcagtc aaaataaaat cattatttgc catccagctg
     2161 atatccccta tagtgagtcg tattacatgg tcatagctgt ttcctggcag ctctggcccg
     2221 tgtctcaaaa tctctgatgt tacattgcac aagataaaaa tatatcatca tgcctcctct
     2281 agaccagcca ggacagaaat gcctcgactt cgctgctacc caaggttgcc gggtgacgca
     2341 caccgtggaa acggatgaag gcacgaaccc agtggacata agcctgttcg gttcgtaagc
     2401 tgtaatgcaa gtagcgtatg cgctcacgca actggtccag aaccttgacc gaacgcagcg
     2461 gtggtaacgg cgcagtggcg gttttcatgg cttgttatga ctgttttttt ggggtacagt
     2521 ctatgcctcg ggcatccaag cagcaagcgc gttacgccgt gggtcgatgt ttgatgttat
     2581 ggagcagcaa cgatgttacg cagcagggca gtcgccctaa aacaaagtta aacattatga
     2641 gggaagcggt gatcgccgaa gtatcgactc aactatcaga ggtagttggc gtcatcgagc
     2701 gccatctcga accgacgttg ctggccgtac atttgtacgg ctccgcagtg gatggcggcc
     2761 tgaagccaca cagtgatatt gatttgctgg ttacggtgac cgtaaggctt gatgaaacaa
     2821 cgcggcgagc tttgatcaac gaccttttgg aaacttcggc ttcccctgga gagagcgaga
     2881 ttctccgcgc tgtagaagtc accattgttg tgcacgacga catcattccg tggcgttatc
     2941 cagctaagcg cgaactgcaa tttggagaat ggcagcgcaa tgacattctt gcaggtatct
     3001 tcgagccagc cacgatcgac attgatctgg ctatcttgct gacaaaagca agagaacata
     3061 gcgttgcctt ggtaggtcca gcggcggagg aactctttga tccggttcct gaacaggatc
     3121 tatttgaggc gctaaatgaa accttaacgc tatggaactc gccgcccgac tgggctggcg
     3181 atgagcgaaa tgtagtgctt acgttgtccc gcatttggta cagcgcagta accggcaaaa
     3241 tcgcgccgaa ggatgtcgct gccgactggg caatggagcg cctgccggcc cagtatcagc
     3301 ccgtcatact tgaagctaga caggcttatc ttggacaaga agaagatcgc ttggcctcgc
     3361 gcgcagatca gttggaagaa tttgtccact acgtgaaagg cgagatcacc aaggtagtcg
     3421 gcaaataacc ctcgagccac ccatgaccaa aatcccttaa cgtgagttac gcgtcgttcc
     3481 actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc
     3541 gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg
     3601 atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa
     3661 atactgttct tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc
     3721 ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt
     3781 gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa
     3841 cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc
     3901 tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc
     3961 cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct
     4021 ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat
     4081 gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc
     4141 tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg
     4201 ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc
     4261 gcagcgagtc agtgagcgag gaagcggaag agcgcccaat acgcaaaccg cctctccccg
     4321 cgcgttggcc gattcattaa tgcagctggc acgacaggtt tcccgactgg aaagcgggca
     4381 gtgagcgcaa cgcaattaat acgcgtaccg ctagccagga agagtttgta gaaacgcaaa
     4441 aaggccatcc gtcaggatgg ccttctgctt agtttgatgc ctggcagttt atggcgggcg
     4501 tcctgcccgc caccctccgg gccgttgctt cacaacgttc aaatccgctc ccggcggatt
     4561 tgtcctactc aggagagcgt tcaccgacaa acaacagata aaacgaaagg cccagtcttc
     4621 cgactgagcc tttcgtttta tttgatgcct
//
