LOCUS       sequence-163135-         912 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Gi-DREADD (P2A) nuclear dTomato expression in forebrain GABA-ergic 
            interneurons under the control of the hDlx enhancer element.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 912)
  AUTHORS   Dimidschstein J, Chen Q, Tremblay R, Rogers SL, Saldi GA, Guo L, Xu 
            Q, Liu R, Lu C, Chu J, Avery MC, Rashid MS, Baek M, Jacob AL, Smith 
            GB, Wilson DE, Kosche G, Kruglikov I, Rusielewicz T, Kotak VC, 
            Mowery TM, Anderson SA, Callaway EM, Dasen JS, Fitzpatrick D, 
            Fossati V, Long MA, Noggle S, Reynolds JH, Sanes DH, Rudy B, Feng G,
            Fishell G
  TITLE     A viral strategy for targeting and manipulating interneurons across 
            vertebrate species.
  JOURNAL   Nat Neurosci. 2016 Oct 31. doi: 10.1038/nn.4430.
  PUBMED    27798629
REFERENCE   2  (bases 1 to 912)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Neurosci. 2016 Oct 31. doi: 10.1038/nn.4430."
COMMENT     Sequence Label: pAAV-hDlx-GiDREADD-dTomato-Fishell-5
FEATURES             Location/Qualifiers
     source          1..912
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(94..792)
                     /codon_start=1
                     /product="dimeric variant of DsRed fluorescent protein 
                     (Shaner et al., 2004)"
                     /label=dTomato
                     /note="mammalian codon-optimized"
                     /translation="VSKGEEVIKEFMRFKVRMEGSMNGHEFEIEGEGEGRPYEGTQTAK
                     LKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYKKLSFPEGFKWERVMNFEDGGL
                     VTVTQDSSLQDGTLIYKVKMRGTNFPPDGPVMQKKTMGWEASTERLYPRDGVLKGEIHQ
                     ALKLKDGGHYLVEFKTIYMAKKPVQLPGYYYVDTKLDITSHNEDYTIVEQYERSEGRHH
                     LFLYGMDELYK"
     primer_bind     complement(177..200)
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
     primer_bind     complement(375..394)
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     644..662
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     CDS             complement(793..813)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             complement(826..882)
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
ORIGIN
        1 agtcaatctt tcacaaattt tgtaatccag aggttgatta tcgataagct tgatatcgaa
       61 ttccctgcag gggtggggcg cgccgtcgac ttacttgtac agctcgtcca tgccgtacag
      121 gaacaggtgg tggcggccct cggagcgctc gtactgttcc acgatggtgt agtcctcgtt
      181 gtgggaggtg atgtccagct tggtgtccac gtagtagtag ccgggcagtt gcacgggctt
      241 cttggccatg tagatggtct tgaactccac caggtagtgg ccgccgtcct tcagcttcag
      301 ggcctggtgg atctcgccct tcagcacgcc gtcgcggggg tacaggcgct cggtggaggc
      361 ctcccagccc atggtcttct tctgcattac ggggccgtcg ggggggaagt tggtgccgcg
      421 catcttcacc ttgtagatca gcgtgccgtc ctgcagggag gagtcctggg tcacggtcac
      481 cagaccgccg tcctcgaagt tcatcacgcg ctcccacttg aagccctcgg ggaaggacag
      541 cttcttgtaa tcggggatgt cggcggggtg cttcacgtac gccttggagc cgtacatgaa
      601 ctggggggac aggatgtccc aggcgaaggg cagggggccg cccttggtca ccttcagctt
      661 ggcggtctgg gtgccctcgt aggggcggcc ctcgccctcg ccctcgatct cgaactcgtg
      721 gccgttcatg gagccctcca tgcgcacctt gaagcgcatg aactctttga tgacctcctc
      781 gcccttgctc accaccttcc tcttcttctt gggggccatt ctagatggcc cgggattctc
      841 ttcgacatcc cctgcttgtt tcaacaggga gaagttagtg gctccgcttc cgttatctcc
      901 ggaccgcctg gc
//
