LOCUS       sequence-163138-         822 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  3' entry vector containing HA epitope fused to C-terminus of mCherry
            without pA for epitope labeling/purification.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 822)
  AUTHORS   Fowler DK, Stewart S, Seredick S, Eisen JS, Stankunas K, Washbourne 
            P
  TITLE     A MultiSite Gateway Toolkit for Rapid Cloning of Vertebrate 
            Expression Constructs with Diverse Research Applications.
  JOURNAL   PLoS One. 2016 Aug 8;11(8):e0159277. doi: 
            10.1371/journal.pone.0159277. eCollection 2016.
  PUBMED    27500400
REFERENCE   2  (bases 1 to 822)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2016-08-8"; volume: "11(8):e0159277. doi"; pages: " 
            10.1371/journal.pone.0159277. eCollection 2016"
COMMENT     Sequence Label: p3E-HA no-pA
FEATURES             Location/Qualifiers
     source          1..822
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(46..169)
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             184..210
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     protein_bind    complement(285..380)
                     /gene="mutant version of attL"
                     /label=attL3
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(417..436)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(418..436)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(441..457)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(441..457)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(559..578)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     primer_bind     complement(637..656)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
ORIGIN
        1 gagctcgaat tctacaggtc actaatacca tctaagtagt tggttcatag tgactgcata
       61 tgttgtgttt tacagtatta tgtagtctgt tttttatgca aaatctaatt taatatattg
      121 atatttatat cattttacgt ttctcgttca actttcttgt acaaagtggg ggatccactc
      181 gagtatccgt acgacgtacc agactacgca gcatagggta ccagtactaa caattgcatt
      241 cattttatgt ttcaggttca gggggaggtg tgggaggttt tttccaactt tattatacaa
      301 agttggcatt ataaaaaagc attgcttatc aatttgttgc aacgaacagg tcactatcag
      361 tcaaaataaa atcattattt ggagctccat ggtagcgtta acgcggccgc gatatcccct
      421 atagtgagtc gtattacatg gtcatagctg tttcctggca gctctggccc gtgtctcaaa
      481 atctctgatg ttacattgca caagataaaa atatatcatc atgaacaata aaactgtctg
      541 cttacataaa cagtaataca aggggtgtta tgagccatat tcaacgggaa acgtcgaggc
      601 cgcgattaaa ttccaacatg gatgctgatt tatatgggta taaatgggct cgcgataatg
      661 tcgggcaatc aggtgcgaca atctatcgct tgtatgggaa gcccgatgcg ccagagttgt
      721 ttctgaaaca tggcaaaggt agcgttgcca atgatgttac agatgagatg gtcagactaa
      781 actggctgac ggaatttatg cctcttccga ccatcaagca tt
//
