LOCUS       sequence-163140-        1074 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Gq-DREADD (P2A) nuclear dTomato expression in forebrain GABA-ergic 
            interneurons under the control of the hDlx enhancer element.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1074)
  AUTHORS   Dimidschstein J, Chen Q, Tremblay R, Rogers SL, Saldi GA, Guo L, Xu 
            Q, Liu R, Lu C, Chu J, Avery MC, Rashid MS, Baek M, Jacob AL, Smith 
            GB, Wilson DE, Kosche G, Kruglikov I, Rusielewicz T, Kotak VC, 
            Mowery TM, Anderson SA, Callaway EM, Dasen JS, Fitzpatrick D, 
            Fossati V, Long MA, Noggle S, Reynolds JH, Sanes DH, Rudy B, Feng G,
            Fishell G
  TITLE     A viral strategy for targeting and manipulating interneurons across 
            vertebrate species.
  JOURNAL   Nat Neurosci. 2016 Oct 31. doi: 10.1038/nn.4430.
  PUBMED    27798629
REFERENCE   2  (bases 1 to 1074)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Neurosci. 2016 Oct 31. doi: 10.1038/nn.4430."
COMMENT     Sequence Label: pAAV-hDlx-GqDREADD-dTomato-Fishell-4
FEATURES             Location/Qualifiers
     source          1..1074
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(41..61)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             complement(74..130)
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
ORIGIN
        1 agcgcatgaa ctctttgatg acctcctcgc ccttgctcac caccttcctc ttcttcttgg
       61 gggccattct agatggcccg ggattctctt cgacatcccc tgcttgtttc aacagggaga
      121 agttagtggc tccgcttccg ttatctccgg acaaggcctg ctcgggtgcg cgcttgtgaa
      181 aaatgaccga ctgtctctgc tggtactgct gcttgcgcct cttttttttg tcacactggc
      241 acagcagcag catcttgaaa gtggttctga atgttttgtt gcacagagca tagcacacgg
      301 ggttcacggt gctgttgatg tagcacagcc agtagcccag attccaaaag gttttgggta
      361 tgcagctgtc acaaaaggtg ttcaccagaa ccatgatgtt gtatggggtc caagtgatga
      421 tgaaggcaag caagatcgca ctgagggtct gggccgcttt cttctccttg accagggaca
      481 tccttttccg cttagtgatc tgacttctgg tcttcagagc aaacctcttg gccagagtgg
      541 cttccttgaa ggacagaggt agagtggccg tgctcttacc cactgaggag ttgacgtcag
      601 aagtcttagc tgtgtccacg gctgactcta gctggatggg aagcttggag aagctttttg
      661 gaaaactgcc tccatcgtcc acgctcttct gggcctgcag cttgtcggct ttcctctcca
      721 agtccaccat ccccagctcc tcctcaggca cctgcaggtt gtccgatgag ggtaacttgg
      781 tggagttgag gatggtgctg tgacccggaa gcttgagcac gatggagtag atggctctcg
      841 tctcggagcc aatgtcctcc tcgtcggagg aggcggagtt ctccagggag gcagcagcat
      901 cattgttgtt ccaactgtca ctgctgctgt ggtcttggtc catctgctcg gagctgggtt
      961 tccagctctt ggttgtgaac cagaagtggc agcggccata cttcctcctg ttggagcgtt
     1021 tcatgctttg ctgttgaagt tcgtaactgc tgcagcttcg agaactgccc gtgg
//
